RPOS_SALTY
ID RPOS_SALTY Reviewed; 330 AA.
AC P0DM81; P0A2E5; P37400; P39699; Q56132; Q93V26; Q9RN87; Q9RN91; Q9RN92;
AC Q9RN93;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; Synonyms=katF;
GN OrderedLocusNames=STM2924;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C52;
RX PubMed=7961444; DOI=10.1128/jb.176.22.6852-6860.1994;
RA Kowarz L., Coynault C., Robbe-Saule V., Norel F.;
RT "The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence,
RT and regulation of spvR and spvABCD virulence plasmid genes.";
RL J. Bacteriol. 176:6852-6860(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1595, 1674, 1704, 1706, and 1747;
RX PubMed=10913067; DOI=10.1128/jb.182.16.4375-4379.2000;
RA Sutton A., Buencamino R., Eisenstark A.;
RT "rpoS mutants in archival cultures of Salmonella enterica serovar
RT typhimurium.";
RL J. Bacteriol. 182:4375-4379(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X77752; CAA54798.1; -; Genomic_DNA.
DR EMBL; AF184103; AAF01234.1; -; Genomic_DNA.
DR EMBL; AF184099; AAF01230.1; -; Genomic_DNA.
DR EMBL; AF184104; AAF01235.1; -; Genomic_DNA.
DR EMBL; AF184098; AAF01229.1; -; Genomic_DNA.
DR EMBL; AF184097; AAF01228.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21804.1; -; Genomic_DNA.
DR PIR; S47534; S47534.
DR RefSeq; NP_461845.1; NC_003197.2.
DR RefSeq; WP_000081498.1; NC_003197.2.
DR AlphaFoldDB; P0DM81; -.
DR SMR; P0DM81; -.
DR STRING; 99287.STM2924; -.
DR PaxDb; P0DM81; -.
DR EnsemblBacteria; AAL21804; AAL21804; STM2924.
DR GeneID; 1254447; -.
DR GeneID; 58317824; -.
DR GeneID; 64173183; -.
DR GeneID; 67517453; -.
DR KEGG; stm:STM2924; -.
DR PATRIC; fig|99287.12.peg.3078; -.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; RRVQREF; -.
DR PhylomeDB; P0DM81; -.
DR BioCyc; SENT99287:STM2924-MON; -.
DR PHI-base; PHI:2683; -.
DR PRO; PR:P0DM81; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..330
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000093973"
FT DNA_BIND 288..307
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 56..89
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 94..164
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 174..249
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 262..315
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 118..121
FT /note="Interaction with polymerase core subunit RpoC"
FT VARIANT 33
FT /note="E -> D (in strain: 1674)"
FT VARIANT 132
FT /note="E -> Q (in strain: 1706)"
FT VARIANT 166..169
FT /note="Missing (in strain: 1747)"
FT VARIANT 299
FT /note="R -> C (in strain: 1595)"
SQ SEQUENCE 330 AA; 37933 MW; 4400E48DEB358ECC CRC64;
MSQNTLKVHD LNEDAEFDEN GVEAFDEKAL SEEEPSDNDL AEEELLSQGA TQRVLDATQL
YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI
EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR
TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD
EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
RVRQIQVEGL RRLREILQTQ GLNIEALFRE
