RPOS_SHIFL
ID RPOS_SHIFL Reviewed; 330 AA.
AC P35540;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959};
GN OrderedLocusNames=SF2764, S2957;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Small P.L., Slonczewski J., Welty D., Falkow S.;
RL Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA03294.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAN44253.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AE014073; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U00119; AAA03294.1; ALT_INIT; Unassigned_DNA.
DR EMBL; AE005674; AAN44253.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE014073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_708546.1; NC_004337.2.
DR RefSeq; WP_000081553.1; NZ_WPGW01000039.1.
DR AlphaFoldDB; P35540; -.
DR SMR; P35540; -.
DR STRING; 198214.SF2764; -.
DR EnsemblBacteria; AAN44253; AAN44253; SF2764.
DR GeneID; 1027440; -.
DR KEGG; sfl:SF2764; -.
DR KEGG; sft:NCTC1_03042; -.
DR PATRIC; fig|198214.7.peg.3290; -.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OrthoDB; 1565734at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..330
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000093974"
FT DNA_BIND 288..307
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 56..89
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 94..164
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 174..249
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 262..315
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 118..121
FT /note="Interaction with polymerase core subunit RpoC"
FT CONFLICT 11
FT /note="L -> I (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 29
FT /note="A -> P (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="D -> Y (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 53
FT /note="R -> L (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="E -> K (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="A -> V (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 191
FT /note="H -> Y (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 196
FT /note="E -> V (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="L -> I (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 308
FT /note="E -> Q (in Ref. 1; AAA03294)"
FT /evidence="ECO:0000305"
FT CONFLICT 328..330
FT /note="FRK -> LPRVSKDLSERPLSSEAGFFCAQ (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 330 AA; 37972 MW; 049E221B5CB99BF1 CRC64;
MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEEEPSDNDL AEEELLSQGA TQRVLDATQL
YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI
EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR
TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD
EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE
RVRQIQVEGL RRLREILQTQ GLNIEALFRK
