RPOS_VIBCH
ID RPOS_VIBCH Reviewed; 335 AA.
AC O51804; Q9JQ13;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959};
DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959};
GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; OrderedLocusNames=VC_0534;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=El Tor Inaba 92A1552;
RX PubMed=9473029; DOI=10.1128/jb.180.4.773-784.1998;
RA Yildiz F.H., Schoolnik G.K.;
RT "Role of rpoS in stress survival and virulence of Vibrio cholerae.";
RL J. Bacteriol. 180:773-784(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the master transcriptional regulator of
CC the stationary phase and the general stress response (By similarity).
CC May be required for the persistence of V.cholerae in aquatic habitats.
CC {ECO:0000255|HAMAP-Rule:MF_00959, ECO:0000269|PubMed:9473029}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00959}.
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DR EMBL; AF000945; AAC38175.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF93702.1; -; Genomic_DNA.
DR PIR; A82312; A82312.
DR RefSeq; NP_230185.1; NC_002505.1.
DR RefSeq; WP_000116737.1; NZ_LT906614.1.
DR AlphaFoldDB; O51804; -.
DR SMR; O51804; -.
DR STRING; 243277.VC_0534; -.
DR PRIDE; O51804; -.
DR DNASU; 2615203; -.
DR EnsemblBacteria; AAF93702; AAF93702; VC_0534.
DR KEGG; vch:VC_0534; -.
DR PATRIC; fig|243277.26.peg.510; -.
DR eggNOG; COG0568; Bacteria.
DR HOGENOM; CLU_014793_3_5_6; -.
DR OMA; RRVQREF; -.
DR BioCyc; VCHO:VC0534-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.10; -; 2.
DR HAMAP; MF_00959; Sigma70_RpoS; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012761; RNA_pol_sigma_RpoS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 2.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02394; rpoS_proteo; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation.
FT CHAIN 1..335
FT /note="RNA polymerase sigma factor RpoS"
FT /id="PRO_0000093976"
FT DNA_BIND 289..308
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 57..90
FT /note="Sigma-70 factor domain-1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 95..165
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 175..250
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT REGION 263..316
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959"
FT MOTIF 119..122
FT /note="Interaction with polymerase core subunit RpoC"
SQ SEQUENCE 335 AA; 38534 MW; 26512CCE15CE2748 CRC64;
MSVSNTVTKV EEFDFEDEAL EVLETDAELT SDEELVAVEG ASEDVREEFD ASAKSLDATQ
MYLSEIGFSP LLTAEEEVLY ARRALRGDEA ARKRMIESNL RLVVKISRRY SNRGLALLDL
IEEGNLGLIR AVEKFDPERG FRFSTYATWW IRQTIERALM NQTRTIRLPI HVVKELNIYL
RTARELSQRL DHEPTPEEIA LELDRPVDDV TKMLRLNERI SSVDTPIGGD GDKALLDILP
DSHNADPEFS TQDDDIRESL LNWLDELNPK QKEVLARRFG LLGYEPSTLE EVGREINLTR
ERVRQIQVEG LRRLREILVK QGLNMEALFN VEYDN
