RPOT1_NICSY
ID RPOT1_NICSY Reviewed; 1002 AA.
AC Q93Y94; Q8L6K0; Q948R5;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=DNA-directed RNA polymerase 1, mitochondrial;
DE EC=2.7.7.6;
DE AltName: Full=NsRpoT-A;
DE AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 1;
DE Flags: Precursor;
GN Name=RPOT1; Synonyms=RPOT-A;
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000312|EMBL:BAB69431.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=11722843; DOI=10.1016/s0378-1119(01)00729-6;
RA Kobayashi Y., Dokiya Y., Sugiura M., Niwa Y., Sugita M.;
RT "Genomic organization and organ-specific expression of a nuclear gene
RT encoding phage-type RNA polymerase in Nicotiana sylvestris.";
RL Gene 279:33-40(2001).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL Plant J. 30:625-637(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000250|UniProtKB:P00573}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC ECO:0000255|PROSITE-ProRule:PRU10032};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11722843,
CC ECO:0000269|PubMed:12061895}.
CC -!- TISSUE SPECIFICITY: The highest levels of expression are detected in
CC the mature leaves. The level of expression is lowest in the cotyledons.
CC {ECO:0000269|PubMed:11722843}.
CC -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC family. {ECO:0000305}.
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DR EMBL; AB058956; BAB69431.1; -; Genomic_DNA.
DR EMBL; AB058954; BAB69063.1; -; mRNA.
DR EMBL; AJ416568; CAC95019.1; -; mRNA.
DR RefSeq; NP_001289502.1; NM_001302573.1.
DR AlphaFoldDB; Q93Y94; -.
DR SMR; Q93Y94; -.
DR STRING; 4096.XP_009767249.1; -.
DR GeneID; 104218451; -.
DR eggNOG; KOG1038; Eukaryota.
DR Proteomes; UP000189701; Unplaced.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.1320.10; -; 1.
DR Gene3D; 1.10.287.260; -; 1.
DR InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR037159; RNA_POL_N_sf.
DR InterPro; IPR029262; RPOL_N.
DR PANTHER; PTHR10102; PTHR10102; 1.
DR Pfam; PF00940; RNA_pol; 1.
DR Pfam; PF14700; RPOL_N; 1.
DR SMART; SM01311; RPOL_N; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE 2: Evidence at transcript level;
KW DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Transit peptide.
FT TRANSIT 1..21
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 22..1002
FT /note="DNA-directed RNA polymerase 1, mitochondrial"
FT /id="PRO_0000031071"
FT ACT_SITE 703
FT /evidence="ECO:0000250"
FT ACT_SITE 778
FT /evidence="ECO:0000250"
FT ACT_SITE 935
FT /evidence="ECO:0000250"
FT CONFLICT 8
FT /note="Q -> H (in Ref. 2; CAC95019)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="I -> T (in Ref. 1; BAB69063)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="Y -> C (in Ref. 1; BAB69063)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="L -> W (in Ref. 1; BAB69063)"
FT /evidence="ECO:0000305"
FT CONFLICT 321
FT /note="V -> L (in Ref. 2; CAC95019)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1002 AA; 113454 MW; 533FA09C13C68804 CRC64;
MWRYISKQAY SRKFRNSHDS ALLGFSQYSS SFGKTRPLQC LCEESTTNPN LGLSQNSIFS
RISRKVRHLE GICEESSKNP HLGLSQNSLF SSVKGDFRVC GKRGSGSLGF LRSYGSAAEA
IASTSEEDID EIQELIEEMN KENEALKTNL QPKQPKTIGG MGVGKYNLLR RRQIKVETEA
WEEAAKEYQE LLMDMCEQKL APNLPYMKSL FLGWFEPLRD AIAAEQKLCD EGKNRGAYAP
FFDQLPAEMM AVITMHKLMG LLMTGGGTGS ARVVQAASHI GEAIEHEARI HRFLEKTKKS
NALSGDLEDT PGDIMKERER VRKKVKILMK KQKLQQVRKI VKQQDDEKPW GQDNLVKVGC
RLIQILMETA YIQPPNDQLD DCPPDIRPAF VHTLKTVETM KGSRRYGVIQ CDPLVRKGLD
KTARHMVIPY MPMLVPPQSW LGYDKGAYLF LPSYIMRTHG AKQQREAVKR VPKKQLEPVF
QALDTLGNTK WRLNRKVLGI VDRIWASGGR LADLVDREDV PLPEEPDAED EAQIRKWKWK
VKGVKKENCE RHSQRCDIEL KLAVARKMKD EDGFYYPHNL DFRGRAYPMH PYLNHLGSDL
CRGILEFAEG RPLGKSGLRW LKIHLANVYG GGVDKLSYEG RVAFSENHVE DIFDSAERPL
EGKRWWLGAE DPFQCLATCI NIAEALRSPS PETAISYMPI HQDGSCNGLQ HYAALGRDTL
GAAAVNLVAG DKPADVYSGI AARVLDIMKR DAAKDPANDP NVMRARLLIN QVDRKLVKQT
VMTSVYGVTY IGARDQIKRR LKERGVIEDD NELFAAACYA AKTTLTALGE MFEAARSIMS
WLGDCAKIIA MENHPVRWTT PLGLPVVQPY RKLGRHLIKT SLQILTLQRE TDKVMVKRQR
TAFPPNFVHS LDGSHMMMTA IACKESGLSF AGVHDSYWTH ASDVDQMNKI LREKFVELYD
APILENLLES FQQSFPDLQF PPLPERGDFD LREVLESPYF FN
