ID   RPOT2_NICSY             Reviewed;        1020 AA.
AC   Q8VWF8; Q8L6K1;
DT   19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-directed RNA polymerase 2, chloroplastic/mitochondrial;
DE            EC=2.7.7.6;
DE   AltName: Full=NsRpoT-B;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 2;
DE   Flags: Precursor;
GN   Name=RPOT2; Synonyms=RPOT-B;
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096 {ECO:0000312|EMBL:BAB78465.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND ALTERNATIVE INITIATION.
RX   PubMed=11741306; DOI=10.1006/bbrc.2001.6130;
RA   Kobayashi Y., Dokiya Y., Sugita M.;
RT   "Dual targeting of phage-type RNA polymerase to both mitochondria and
RT   plastids is due to alternative translation initiation in single
RT   transcripts.";
RL   Biochem. Biophys. Res. Commun. 289:1106-1113(2001).
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250|UniProtKB:P00573}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Mitochondrion.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=1; Synonyms=Chloroplast/mitochondrial;
CC         IsoId=Q8VWF8-1; Sequence=Displayed;
CC       Name=2; Synonyms=Mitochondrial;
CC         IsoId=Q8VWF8-2; Sequence=VSP_018883;
CC   -!- TISSUE SPECIFICITY: The highest levels of expression are detected in
CC       the mature leaves. The level of expression is lowest in the cotyledons.
CC       {ECO:0000269|PubMed:11741306}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AB058957; BAB78464.1; -; mRNA.
DR   EMBL; AB058958; BAB78465.1; -; Genomic_DNA.
DR   EMBL; AJ302019; CAC82575.2; -; mRNA.
DR   RefSeq; NP_001289520.1; NM_001302591.1. [Q8VWF8-1]
DR   AlphaFoldDB; Q8VWF8; -.
DR   SMR; Q8VWF8; -.
DR   STRING; 4096.XP_009775223.1; -.
DR   GeneID; 104225149; -.
DR   eggNOG; KOG1038; Eukaryota.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0009536; C:plastid; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative initiation; Chloroplast; DNA-directed RNA polymerase;
KW   Mitochondrion; Nucleotidyltransferase; Plastid; Reference proteome;
KW   Transcription; Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast and mitochondrion"
FT   CHAIN           ?..1020
FT                   /note="DNA-directed RNA polymerase 2,
FT                   chloroplastic/mitochondrial"
FT                   /id="PRO_0000031072"
FT   REGION          314..336
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        721
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        796
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        953
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..34
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018883"
FT   CONFLICT        403
FT                   /note="R -> P (in Ref. 1; BAB78464/BAB78465)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        552..553
FT                   /note="RT -> LP (in Ref. 1; BAB78464/BAB78465)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        753..754
FT                   /note="DV -> EA (in Ref. 1; BAB78464/BAB78465)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1020 AA;  116177 MW;  E4867D7631F397B3 CRC64;
     MSSTKTPISL TIKLNQFTDK PTGLDINPYH NSPIMWRNII KQLSSRTPQK LLFSSKNRTY
     SFLGFGQDSI FKDNTKFRSL IPISCSNIVM GFQNLGEYLP GDEFLSRPLI KNQVNNNFCC
     RKSYASVAEA VAVSSTDAEE DVSVVDEVHE LLTELKKEEK KQFAFRRRKQ RMLTSGMGHR
     KYQTLKRRQV KVETEAWEQA AKEYKELLFD MCEQKLAPNL PYVKSLFLGW FEPLRDKIAE
     EQELCSQGKS KAAYAKYFYQ LPADMMAVIT MHKLMGLLMT GGDHGTARVV QAALVIGDAI
     EQEVRIHNFL EKTKKQKAEK DKQKEDGEHV TQEQEKLRKK VTNLMKKQKL RAVGQIVRRQ
     DDSKPWGQDA RAKVGSRLID LLLQTAYIQP PANQLAVDPP DIRPAFVHSV RTVAKETKSA
     SRRYGIIQCD ELVFKGLERT ARHMVIPYMP MLVPPVKWTG YDKGGHLYLP SYVMRTHGAR
     QQREAVKRAS RNQLQPVFEA LDTLGNTKWR INKRVLSVVD RIWAGGGRLA DLVDRDDAPL
     PEEPDTEDEA LRTKWRWKVK SVKKENRERH SQRCDIELKL AVARKMKDEE SFFYPHNVDF
     RGRAYPMHPH LNHLGSDICR GVLEFAEGRP LGESGLRWLK IHLANLFAGG VEKLSLEGRI
     GFTENHMDDI FDSSDKPLEG RRWWLNAEDP FQCLAVCINL SEAVRSSSPE TSVSHIPVHQ
     DGSCNGLQHY AALGRDKLGA AAVNLVAGEK PADVYSGIAA RVLDIMKRDA QRDPAEFPDA
     VRARVLVNQV DRKLVKQTVM TSVYGVTYIG ARDQIKRRLK ERGAIADDSE LFGAACYAAK
     VTLTALGEMF EAARSIMTWL GECAKIIASE NEPVRWTTPL GLPVVQPYRK IGRHLIKTSL
     QILTLQRETE KVMVKRQRTA FPPNFIHSLD GSHMMMTAVA CRRAGLNFAG VHDSYWTHAC
     DVDKLNRILR EKFVELYEAP ILEKLLESFQ TSYPTLLFPP LPERGDFDMR DVLESPYFFN