RPOT3_ARATH
ID RPOT3_ARATH Reviewed; 993 AA.
AC O24600; Q0WN04;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=DNA-directed RNA polymerase 3, chloroplastic;
DE EC=2.7.7.6;
DE AltName: Full=Protein SCABRA 3 {ECO:0000303|PubMed:16698900};
DE Flags: Precursor;
GN Name=RPOT3;
GN Synonyms=RPOPT, RPOTP {ECO:0000303|PubMed:16698900},
GN SCA3 {ECO:0000303|PubMed:16698900}; OrderedLocusNames=At2g24120;
GN ORFNames=F27D4.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=9242608; DOI=10.1126/science.277.5327.809;
RA Hedtke B., Boerner T., Weihe A.;
RT "Mitochondrial and chloroplast phage-type RNA polymerases in Arabidopsis.";
RL Science 277:809-811(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=10205908; DOI=10.1046/j.1365-313x.1999.00393.x;
RA Hedtke B., Meixner M., Gillandt S., Richter E., Boerner T., Weihe A.;
RT "Green fluorescent protein as a marker to investigate targeting of
RT organellar RNA polymerases of higher plants in vivo.";
RL Plant J. 17:557-561(1999).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11258484; DOI=10.1093/embo-reports/kvd086;
RA Hedtke B., Boerner T., Weihe A.;
RT "One RNA polymerase serving two genomes.";
RL EMBO Rep. 1:435-440(2000).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16698900; DOI=10.1104/pp.106.080069;
RA Hricova A., Quesada V., Micol J.L.;
RT "The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which
RT is required for chloroplast biogenesis and mesophyll cell proliferation in
RT Arabidopsis.";
RL Plant Physiol. 141:942-956(2006).
CC -!- FUNCTION: Nuclear-encoded DNA-dependent RNA polymerase that catalyzes
CC the transcription of DNA into RNA in chloroplasts using the four
CC ribonucleoside triphosphates as substrates (Probable). Required for
CC chloroplast development and leaf mesophyll cell proliferation
CC (PubMed:16698900). {ECO:0000269|PubMed:16698900,
CC ECO:0000305|PubMed:16698900}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC ECO:0000255|PROSITE-ProRule:PRU10032};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10205908}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O24600-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Reduced plant growth, impaired lateral root
CC formation, rounded and pale-green leaves, protruding leaf laminae,
CC irregular leaf margins and reduced seed sets.
CC {ECO:0000269|PubMed:16698900}.
CC -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC family. {ECO:0000305}.
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DR EMBL; Y08722; CAA69972.1; -; Genomic_DNA.
DR EMBL; Y08463; CAA69717.1; -; mRNA.
DR EMBL; AC005967; AAD03373.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07531.1; -; Genomic_DNA.
DR EMBL; AK229652; BAF01496.1; -; mRNA.
DR PIR; G84632; G84632.
DR RefSeq; NP_179989.1; NM_127973.5. [O24600-1]
DR AlphaFoldDB; O24600; -.
DR SMR; O24600; -.
DR BioGRID; 2298; 1.
DR IntAct; O24600; 1.
DR STRING; 3702.AT2G24120.1; -.
DR PaxDb; O24600; -.
DR PRIDE; O24600; -.
DR ProteomicsDB; 228193; -. [O24600-1]
DR EnsemblPlants; AT2G24120.1; AT2G24120.1; AT2G24120. [O24600-1]
DR GeneID; 816946; -.
DR Gramene; AT2G24120.1; AT2G24120.1; AT2G24120. [O24600-1]
DR KEGG; ath:AT2G24120; -.
DR Araport; AT2G24120; -.
DR TAIR; locus:2047515; AT2G24120.
DR eggNOG; KOG1038; Eukaryota.
DR HOGENOM; CLU_003364_4_0_1; -.
DR InParanoid; O24600; -.
DR OrthoDB; 135329at2759; -.
DR PhylomeDB; O24600; -.
DR PRO; PR:O24600; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O24600; baseline and differential.
DR Genevisible; O24600; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR Gene3D; 1.10.1320.10; -; 1.
DR Gene3D; 1.10.287.260; -; 1.
DR InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR037159; RNA_POL_N_sf.
DR InterPro; IPR029262; RPOL_N.
DR PANTHER; PTHR10102; PTHR10102; 1.
DR Pfam; PF00940; RNA_pol; 1.
DR Pfam; PF14700; RPOL_N; 1.
DR SMART; SM01311; RPOL_N; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; DNA-directed RNA polymerase;
KW Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW Transferase; Transit peptide.
FT TRANSIT 1..95
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 96..993
FT /note="DNA-directed RNA polymerase 3, chloroplastic"
FT /id="PRO_0000031079"
FT ACT_SITE 694
FT /evidence="ECO:0000250"
FT ACT_SITE 769
FT /evidence="ECO:0000250"
FT ACT_SITE 926
FT /evidence="ECO:0000250"
SQ SEQUENCE 993 AA; 112624 MW; BFBBC748DE81445D CRC64;
MASAAAASPS LSLNPTSHFQ HQTSLVTWLK PPPSSALFRR KTLPFFERHS LPISASSSSS
SSSSTSLSVH EKPISNSVHF HGNLIESFEN QDSSYAGTIK GASLIEELEN PVERNGLSGR
RRLFMQDPPW ISALFLKGLS KMVDQTLKIE RKDIDKRKFD SLRRRQVKEE TEAWERMVDE
YRDLEKEMCE KNLAPNLPYV KHMFLGWFQP LKDVIEREQK LQKNKSKKVR AAYAPHIELL
PADKMAVIVM HKMMGLVMSG HEDGCIQVVQ AAVSIGIAIE QEVRIHNFLK RTRKNNAGDS
QEELKEKQLL RKRVNSLIRR KRIIDALKVV KSEGTKPWGR ATQAKLGSRL LELLIEAAYV
QPPLTQSGDS IPEFRPAFRH RFKTVTKYPG SKLVRRYGVI ECDSLLLAGL DKSAKHMLIP
YVPMLVPPKR WKGYDKGGYL FLPSYIMRTH GSKKQQDALK DISHKTAHRV FEALDTLGNT
KWRVNRNILD VVERLWADGG NIAGLVNRED VPIPEKPSSE DPEELQSWKW SARKANKINR
ERHSLRCDVE LKLSVARKMK DEEGFYYPHN LDFRGRAYPM HPHLNHLSSD LCRGTLEFAE
GRPLGKSGLH WLKIHLANLY AGGVEKLSHD ARLAFVENHL DDIMDSAENP IHGKRWWLKA
EDPFQCLAAC VILTQALKSP SPYSVISHLP IHQDGSCNGL QHYAALGRDS FEAAAVNLVA
GEKPADVYSE ISRRVHEIMK KDSSKDPESN PTAALAKILI TQVDRKLVKQ TVMTSVYGVT
YVGAREQIKR RLEEKGVITD ERMLFAAACY SAKVTLAALG EIFEAARAIM SWLGDCAKII
ASDNHPVRWI TPLGLPVVQP YCRSERHLIR TSLQVLALQR EGNTVDVRKQ RTAFPPNFVH
SLDGTHMMMT AVACREAGLN FAGVHDSYWT HACDVDTMNR ILREKFVELY NTPILEDLLQ
SFQESYPNLV FPPVPKRGDF DLKEVLKSQY FFN
