ID   RPOY_GEOS3              Reviewed;          71 AA.
AC   A0A0K2H5X8;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   11-NOV-2015, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=DNA-directed RNA polymerase subunit epsilon {ECO:0000255|HAMAP-Rule:MF_01553, ECO:0000303|PubMed:25092033};
DE            Short=RNAP epsilon subunit {ECO:0000255|HAMAP-Rule:MF_01553};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01553};
DE   AltName: Full=RNA polymerase epsilon subunit {ECO:0000255|HAMAP-Rule:MF_01553};
DE   AltName: Full=Transcriptase subunit epsilon {ECO:0000255|HAMAP-Rule:MF_01553};
GN   Name=rpoY {ECO:0000255|HAMAP-Rule:MF_01553, ECO:0000303|PubMed:25092033};
GN   ORFNames=GT50_03365;
OS   Geobacillus stearothermophilus (strain DSM 13240 / CIP 106956 / 10).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=272567;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 13240 / CIP 106956 / 10;
RA   Lewis S.A., Clifton S.W., Najar F.Z., Roe B.A.;
RT   "Complete genome Sequence of Geobacillus stearothermophilus strain 10, a
RT   Yellowstone hot spring isolate.";
RL   Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-58, AND SUBUNIT.
RX   PubMed=25092033; DOI=10.1128/jb.02020-14;
RA   Keller A.N., Yang X., Wiedermannova J., Delumeau O., Krasny L., Lewis P.J.;
RT   "epsilon, a new subunit of RNA polymerase found in gram-positive
RT   bacteria.";
RL   J. Bacteriol. 196:3622-3632(2014).
CC   -!- FUNCTION: A non-essential component of RNA polymerase (RNAP)
CC       (Probable). Has a similar structure to bacteriophage T7 protein Gp2 (AC
CC       P03704), which is known to bind to RNAP in the DNA binding-cleft.
CC       Unlike Gp2 however, this protein does not inhibit transcription
CC       inititation (PubMed:25092033). {ECO:0000269|PubMed:25092033,
CC       ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01553};
CC   -!- SUBUNIT: Monomer (PubMed:25092033). RNAP is composed of a core of 2
CC       alpha, a beta and a beta' subunit. The core is associated with a delta
CC       subunit, and at least one of epsilon or omega. When a sigma factor is
CC       associated with the core the holoenzyme is formed, which can initiate
CC       transcription (By similarity). {ECO:0000250|UniProtKB:O31718,
CC       ECO:0000269|PubMed:25092033}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase subunit epsilon family.
CC       {ECO:0000255|HAMAP-Rule:MF_01553}.
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DR   EMBL; CP008934; ALA69333.1; -; Genomic_DNA.
DR   RefSeq; WP_013145900.1; NZ_CP008934.1.
DR   PDB; 4NJC; X-ray; 2.30 A; A/B/C/D/E/F/G/H=1-58.
DR   PDBsum; 4NJC; -.
DR   AlphaFoldDB; A0A0K2H5X8; -.
DR   SMR; A0A0K2H5X8; -.
DR   EnsemblBacteria; ALA69333; ALA69333; GT50_03365.
DR   KEGG; gse:GT50_03365; -.
DR   PATRIC; fig|272567.8.peg.680; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01553; RNApol_bact_RpoY; 1.
DR   InterPro; IPR009907; RpoY.
DR   Pfam; PF07288; RpoY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Transcription; Transferase.
FT   CHAIN           1..71
FT                   /note="DNA-directed RNA polymerase subunit epsilon"
FT                   /id="PRO_0000451559"
FT   STRAND          1..9
FT                   /evidence="ECO:0007829|PDB:4NJC"
FT   STRAND          20..29
FT                   /evidence="ECO:0007829|PDB:4NJC"
FT   HELIX           30..37
FT                   /evidence="ECO:0007829|PDB:4NJC"
FT   STRAND          40..50
FT                   /evidence="ECO:0007829|PDB:4NJC"
FT   STRAND          53..56
FT                   /evidence="ECO:0007829|PDB:4NJC"
SQ   SEQUENCE   71 AA;  8444 MW;  BCA811D6D703E51D CRC64;
     MIFKVFYQEN ADEVPVREKT KTLYIEAESE RDVRRKLEGR PINIEYIQPL EGAHLEYEKK
     SPNFQVLEIS S