ATXA_BACAN
ID ATXA_BACAN Reviewed; 475 AA.
AC Q44636; Q84J36; Q933U4; Q933V7; Q9RQT9;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Anthrax toxin expression trans-acting positive regulator;
GN Name=atxA; OrderedLocusNames=pXO1-119, BXA0146, GBAA_pXO1_0146;
OS Bacillus anthracis.
OG Plasmid pXO1.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=UM23-1;
RX PubMed=8366021; DOI=10.1128/jb.175.17.5329-5338.1993;
RA Uchida I., Hornung J.M., Thorne C.B., Klimpel K.R., Leppla S.H.;
RT "Cloning and characterization of a gene whose product is a trans-activator
RT of anthrax toxin synthesis.";
RL J. Bacteriol. 175:5329-5338(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RX PubMed=10515943; DOI=10.1128/jb.181.20.6509-6515.1999;
RA Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P.,
RA Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y.,
RA Ricke D., Svensson R., Jackson P.J.;
RT "Sequence and organization of pXO1, the large Bacillus anthracis plasmid
RT harboring the anthrax toxin genes.";
RL J. Bacteriol. 181:6509-6515(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Ames / isolate Florida / A2012;
RX PubMed=12004073; DOI=10.1126/science.1071837;
RA Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA Fraser C.M.;
RT "Comparative genome sequencing for discovery of novel polymorphisms in
RT Bacillus anthracis.";
RL Science 296:2028-2033(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 8-475.
RC STRAIN=Carbosap, and Ferrara;
RX PubMed=12067380; DOI=10.1046/j.1365-2672.2002.01660.x;
RA Adone R., Pasquali P., La Rosa G., Marianelli C., Muscillo M.,
RA Fasanella A., Francia M., Ciuchini F.;
RT "Sequence analysis of the genes encoding for the major virulence factors of
RT Bacillus anthracis vaccine strain 'Carbosap'.";
RL J. Appl. Microbiol. 93:117-121(2002).
RN [6]
RP CHARACTERIZATION.
RX PubMed=9234759; DOI=10.1128/iai.65.8.3091-3099.1997;
RA Hoffmaster A.R., Koehler T.M.;
RT "The anthrax toxin activator gene atxA is associated with CO2-enhanced non-
RT toxin gene expression in Bacillus anthracis.";
RL Infect. Immun. 65:3091-3099(1997).
CC -!- FUNCTION: Acts in trans to stimulate anthrax toxins expression. It
CC activates the transcription of the pagA, cya and lef genes. It also
CC activates capB, a gene required for capsule production.
CC -!- INDUCTION: By carbonic acid and CO(2).
CC -!- SIMILARITY: Belongs to the AtxA/AcpA family. {ECO:0000305}.
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DR EMBL; L13841; AAA71986.1; -; Unassigned_DNA.
DR EMBL; AF065404; AAD32423.1; -; Genomic_DNA.
DR EMBL; AE011190; AAM26091.1; -; Genomic_DNA.
DR EMBL; AE017336; AAT28886.2; -; Genomic_DNA.
DR EMBL; AJ413928; CAC93922.1; -; Genomic_DNA.
DR EMBL; AJ413929; CAC93923.1; -; Genomic_DNA.
DR PIR; A48664; A48664.
DR PIR; G59105; G59105.
DR RefSeq; NP_052815.1; NC_001496.1.
DR RefSeq; WP_000957312.1; NZ_VTZH01000015.1.
DR PDB; 4R6I; X-ray; 2.65 A; A/B=1-475.
DR PDBsum; 4R6I; -.
DR AlphaFoldDB; Q44636; -.
DR SMR; Q44636; -.
DR EnsemblBacteria; AAT28886; AAT28886; GBAA_pXO1_0146.
DR GeneID; 45025503; -.
DR KEGG; bar:GBAA_pXO1_0146; -.
DR HOGENOM; CLU_569549_0_0_9; -.
DR OMA; RITHIIQ; -.
DR PHI-base; PHI:4087; -.
DR Proteomes; UP000000594; Plasmid pXO1.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR013196; HTH_11.
DR InterPro; IPR007737; Mga_HTH.
DR InterPro; IPR011608; PRD.
DR InterPro; IPR036634; PRD_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF08279; HTH_11; 1.
DR Pfam; PF05043; Mga; 1.
DR Pfam; PF00874; PRD; 2.
DR SUPFAM; SSF63520; SSF63520; 1.
DR PROSITE; PS51372; PRD_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Capsule biogenesis/degradation; Plasmid;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Virulence.
FT CHAIN 1..475
FT /note="Anthrax toxin expression trans-acting positive
FT regulator"
FT /id="PRO_0000219545"
FT DOMAIN 169..274
FT /note="PRD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT DOMAIN 282..390
FT /note="PRD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT VARIANT 75
FT /note="L -> LL (in strain: UM23-1)"
FT VARIANT 387
FT /note="F -> Y (in strain: UM23-1)"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 10..23
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 30..37
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 41..54
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 80..89
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 91..101
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 106..112
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 117..130
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 131..134
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:4R6I"
FT TURN 140..143
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 144..147
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 149..159
FT /evidence="ECO:0007829|PDB:4R6I"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 175..189
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 195..213
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 223..225
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 232..248
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 254..267
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 277..302
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 310..325
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 336..345
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 347..358
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 362..365
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 370..389
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 392..397
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 402..416
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 417..419
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 420..422
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 432..436
FT /evidence="ECO:0007829|PDB:4R6I"
FT TURN 437..439
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 442..447
FT /evidence="ECO:0007829|PDB:4R6I"
FT STRAND 456..458
FT /evidence="ECO:0007829|PDB:4R6I"
FT HELIX 465..474
FT /evidence="ECO:0007829|PDB:4R6I"
SQ SEQUENCE 475 AA; 55562 MW; FD361F1795CD9E26 CRC64;
MLTPISIEKE HIRLINLLHF INEQNRWFTI KELSDYLQVA DKTVRKYLKL LEDEIPPSWN
LLVQKGKGIY LKKPLNESLS FVESKILRKS LNLQICEELV FKKNSMQSLA QKLHLQVGAL
YPIINQINYD IQSSHLNIKK KPLEISGREQ DVRVFMLRLY CNIPNDYWPF PYINKQNITD
LINKMEKILN VQMYTYSKHK LCVLFAITIS RLLSGNTIDN VSGLILVNKN DDHYKTVASI
TSELQNSFGV TLHETEISFL ALALLLSLGN SITTDSNKTL TSYKKTIMPL AKEITKGIEH
KLQLGINYDE SFLTYVVLII KKALDKNFIQ YYNYNIKFIR HIKQRHPNTF NTIQECISNL
NYTVYSHFDC YEISLLTMHF ETQRMLFKNN PKKIYVYTSQ GCIHREYISA LLEKRYNGLI
KIVRNTIINL TNESLQDMEI DIIISNVNLP IKNIPIVQIS EFPTERDFHE IKKII
