ID   ATXA_STRGL              Reviewed;         143 AA.
AC   P01551;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Actinoxanthin;
DE            Short=AXN;
DE   Flags: Precursor;
GN   Name=axnA;
OS   Streptomyces globisporus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1908;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8226326; DOI=10.7164/antibiotics.46.1475;
RA   Sakata N., Mase S., Ikeno S., Hori M., Otani T.;
RT   "The amino acid sequence of actinoxanthin apoprotein deduced from the base
RT   sequence of the gene.";
RL   J. Antibiot. 46:1475-1477(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 34-143.
RX   PubMed=994323; DOI=10.7164/antibiotics.29.1026;
RA   Khokhlov A.S., Reshetov P.D., Chupova L.A., Cherches B.Z., Zhigis L.S.,
RA   Stoyachenko I.A.;
RT   "Chemical studies on actinoxanthin.";
RL   Jpn. J. Antibiot. 29:1026-1034(1976).
RN   [3]
RP   DISULFIDE BONDS.
RA   Zhigis L.S., Stoyachenko I.A., Cherches B.Z., Reshetov P.D., Khokhlov A.S.;
RT   "Actinoxanthin. VII. Location of disulfide bonds.";
RL   Bioorg. Khim. 2:506-510(1976).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS).
RA   Pletnev V.Z., Kuzin A.P., Trakhanov S.D., Kostetsky P.V., Popovich V.A.,
RA   Tsigannik I.N.;
RT   "Three-dimensional structure of actinoxanthin. III. A 4-A resolution.";
RL   Biopolymers 20:679-694(1981).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RA   Pletnev V.Z., Kuzin A.P., Trakhanov S.D., Kostetsky P.V.;
RT   "Three-dimensional structure of actinoxanthin. IV. A 2.5-A resolution.";
RL   Biopolymers 21:287-300(1982).
CC   -!- FUNCTION: Binds non-covalently to a chromophore which is the cytotoxic
CC       and mutagenic component of the antibiotic. The chromophore binds to DNA
CC       as a weak intercalator and causes single- and double-strand breaks.
CC   -!- SIMILARITY: Belongs to the neocarzinostatin family. {ECO:0000305}.
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DR   EMBL; D11457; BAA02014.1; -; Genomic_DNA.
DR   PDB; 1ACX; X-ray; 2.00 A; A=34-143.
DR   PDBsum; 1ACX; -.
DR   AlphaFoldDB; P01551; -.
DR   BMRB; P01551; -.
DR   SMR; P01551; -.
DR   EvolutionaryTrace; P01551; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR027273; Neocarzinostatin-like.
DR   InterPro; IPR002186; Neocarzinostatin_fam.
DR   Pfam; PF00960; Neocarzinostat; 1.
DR   PRINTS; PR01885; MACROMOMYCIN.
DR   SUPFAM; SSF49319; SSF49319; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; DNA-binding; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000269|PubMed:994323"
FT   CHAIN           34..143
FT                   /note="Actinoxanthin"
FT                   /id="PRO_0000019458"
FT   DISULFID        69..78
FT                   /evidence="ECO:0000269|Ref.3"
FT   DISULFID        119..124
FT                   /evidence="ECO:0000269|Ref.3"
FT   CONFLICT        54..55
FT                   /note="Missing (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        100
FT                   /note="Missing (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        107
FT                   /note="S -> E (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        110
FT                   /note="E -> S (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        134
FT                   /note="D -> N (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   STRAND          36..40
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          50..55
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          62..69
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   TURN            80..82
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          85..87
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          95..99
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          102..107
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          113..118
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   TURN            119..121
FT                   /evidence="ECO:0007829|PDB:1ACX"
FT   STRAND          125..129
FT                   /evidence="ECO:0007829|PDB:1ACX"
SQ   SEQUENCE   143 AA;  13887 MW;  2CB2E57FA5DC2F2C CRC64;
     MSLRHMSRRA SRFGVVAVAS IGLAAAAQSV AFAAPAFSVS PASGLSDGQS VSVSVSGAAA
     GETYYIAQCA PVGGQDACNP ATATSFTTDA SGAASFSFVV RKSYAGSTPE GTPVGSVDCA
     TDACNLGAGN SGLDLGHVAL TFG