RPOZ_MYCS2
ID RPOZ_MYCS2 Reviewed; 107 AA.
AC A0QWT1; I7G8B1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DNA-directed RNA polymerase subunit omega {ECO:0000255|HAMAP-Rule:MF_00366};
DE Short=RNAP omega subunit {ECO:0000255|HAMAP-Rule:MF_00366};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00366};
DE AltName: Full=RNA polymerase omega subunit {ECO:0000255|HAMAP-Rule:MF_00366};
DE AltName: Full=Transcriptase subunit omega {ECO:0000255|HAMAP-Rule:MF_00366};
GN Name=rpoZ {ECO:0000255|HAMAP-Rule:MF_00366};
GN OrderedLocusNames=MSMEG_3053, MSMEI_2977;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=19926651; DOI=10.1099/mic.0.033670-0;
RA Dey A., Verma A.K., Chatterji D.;
RT "Role of an RNA polymerase interacting protein, MsRbpA, from Mycobacterium
RT smegmatis in phenotypic tolerance to rifampicin.";
RL Microbiology 156:873-883(2010).
CC -!- FUNCTION: Promotes RNA polymerase assembly. Latches the N- and C-
CC terminal regions of the beta' subunit thereby facilitating its
CC interaction with the beta and alpha subunits. {ECO:0000255|HAMAP-
CC Rule:MF_00366, ECO:0000269|PubMed:19926651}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00366};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_00366, ECO:0000269|PubMed:19926651}.
CC -!- SIMILARITY: Belongs to the RNA polymerase subunit omega family.
CC {ECO:0000255|HAMAP-Rule:MF_00366}.
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DR EMBL; CP000480; ABK69545.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP39441.1; -; Genomic_DNA.
DR RefSeq; WP_011728783.1; NZ_SIJM01000002.1.
DR RefSeq; YP_887369.1; NC_008596.1.
DR PDB; 5TW1; X-ray; 2.76 A; E=1-107.
DR PDB; 5VI5; X-ray; 3.20 A; E=1-107.
DR PDB; 5VI8; X-ray; 2.76 A; E=1-107.
DR PDB; 6CCE; X-ray; 3.05 A; E=1-107.
DR PDB; 6CCV; X-ray; 3.05 A; E=1-107.
DR PDB; 6DCF; X-ray; 3.45 A; E=1-107.
DR PDB; 6EYD; EM; 4.20 A; E=2-107.
DR PDB; 6F6W; EM; 3.80 A; E=2-107.
DR PDB; 6VVS; X-ray; 3.11 A; E=1-107.
DR PDB; 6VVT; X-ray; 2.90 A; E=1-107.
DR PDB; 6VVV; X-ray; 3.20 A; E=1-107.
DR PDB; 6YXU; EM; 3.08 A; E=1-107.
DR PDB; 6YYS; EM; 3.08 A; E=1-107.
DR PDB; 6Z11; EM; 3.36 A; E=1-107.
DR PDB; 7P5X; EM; 3.20 A; AE=1-107.
DR PDBsum; 5TW1; -.
DR PDBsum; 5VI5; -.
DR PDBsum; 5VI8; -.
DR PDBsum; 6CCE; -.
DR PDBsum; 6CCV; -.
DR PDBsum; 6DCF; -.
DR PDBsum; 6EYD; -.
DR PDBsum; 6F6W; -.
DR PDBsum; 6VVS; -.
DR PDBsum; 6VVT; -.
DR PDBsum; 6VVV; -.
DR PDBsum; 6YXU; -.
DR PDBsum; 6YYS; -.
DR PDBsum; 6Z11; -.
DR PDBsum; 7P5X; -.
DR AlphaFoldDB; A0QWT1; -.
DR SMR; A0QWT1; -.
DR IntAct; A0QWT1; 1.
DR STRING; 246196.MSMEI_2977; -.
DR EnsemblBacteria; ABK69545; ABK69545; MSMEG_3053.
DR EnsemblBacteria; AFP39441; AFP39441; MSMEI_2977.
DR GeneID; 66734458; -.
DR KEGG; msg:MSMEI_2977; -.
DR KEGG; msm:MSMEG_3053; -.
DR PATRIC; fig|246196.19.peg.3014; -.
DR eggNOG; COG1758; Bacteria.
DR OMA; QHADSKY; -.
DR OrthoDB; 1864134at2; -.
DR BRENDA; 2.7.7.6; 3512.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.940.10; -; 1.
DR HAMAP; MF_00366; RNApol_bact_RpoZ; 1.
DR InterPro; IPR003716; DNA-dir_RNA_pol_omega.
DR InterPro; IPR006110; Pol_omega/Rpo6/RPB6.
DR InterPro; IPR036161; RPB6/omega-like_sf.
DR PANTHER; PTHR34476; PTHR34476; 1.
DR Pfam; PF01192; RNA_pol_Rpb6; 1.
DR SMART; SM01409; RNA_pol_Rpb6; 1.
DR SUPFAM; SSF63562; SSF63562; 1.
DR TIGRFAMs; TIGR00690; rpoZ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..107
FT /note="DNA-directed RNA polymerase subunit omega"
FT /id="PRO_1000005960"
FT HELIX 31..33
FT /evidence="ECO:0007829|PDB:5TW1"
FT HELIX 37..41
FT /evidence="ECO:0007829|PDB:5TW1"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:5TW1"
FT HELIX 47..64
FT /evidence="ECO:0007829|PDB:5TW1"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:6VVT"
FT HELIX 88..97
FT /evidence="ECO:0007829|PDB:5TW1"
FT STRAND 101..104
FT /evidence="ECO:0007829|PDB:5TW1"
SQ SEQUENCE 107 AA; 11535 MW; 8CB11B5F01437C7A CRC64;
MSTPHADAQL NAADDLGIDS SAASAYDTPL GITNPPIDEL LSRASSKYAL VIYAAKRARQ
INDYYNQLGD GILEYVGPLV EPGLQEKPLS IALREIHGDL LEHTEGE
