RPOZ_MYCTU
ID RPOZ_MYCTU Reviewed; 110 AA.
AC P9WGY5; L0T993; P66721; P71660;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=DNA-directed RNA polymerase subunit omega;
DE Short=RNAP omega subunit;
DE EC=2.7.7.6;
DE AltName: Full=RNA polymerase omega subunit;
DE AltName: Full=Transcriptase subunit omega;
GN Name=rpoZ; OrderedLocusNames=Rv1390; ORFNames=MTCY21B4.07;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=22570422; DOI=10.1093/nar/gks346;
RA Hu Y., Morichaud Z., Chen S., Leonetti J.P., Brodolin K.;
RT "Mycobacterium tuberculosis RbpA protein is a new type of transcriptional
RT activator that stabilizes the sigma A-containing RNA polymerase
RT holoenzyme.";
RL Nucleic Acids Res. 40:6547-6557(2012).
CC -!- FUNCTION: Promotes RNA polymerase assembly. Latches the N- and C-
CC terminal regions of the beta' subunit thereby facilitating its
CC interaction with the beta and alpha subunits.
CC {ECO:0000269|PubMed:22570422}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000269|PubMed:22570422}.
CC -!- SIMILARITY: Belongs to the RNA polymerase subunit omega family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP44149.1; -; Genomic_DNA.
DR PIR; D70899; D70899.
DR RefSeq; NP_215906.1; NC_000962.3.
DR RefSeq; WP_003407248.1; NZ_NVQJ01000050.1.
DR PDB; 5UH5; X-ray; 3.75 A; E=1-110.
DR PDB; 5UH6; X-ray; 3.84 A; E=1-110.
DR PDB; 5UH8; X-ray; 4.18 A; E=1-110.
DR PDB; 5UH9; X-ray; 4.40 A; E=1-110.
DR PDB; 5UHA; X-ray; 3.91 A; E=1-110.
DR PDB; 5UHB; X-ray; 4.29 A; E=1-110.
DR PDB; 5UHC; X-ray; 3.80 A; E=1-110.
DR PDB; 5UHD; X-ray; 4.01 A; E=1-110.
DR PDB; 5UHE; X-ray; 4.04 A; E=1-110.
DR PDB; 5UHF; X-ray; 4.34 A; E=1-110.
DR PDB; 5UHG; X-ray; 3.97 A; E=1-110.
DR PDB; 5ZX2; X-ray; 2.80 A; E=1-110.
DR PDB; 5ZX3; X-ray; 2.75 A; E=1-110.
DR PDB; 6BZO; EM; 3.38 A; E=2-110.
DR PDB; 6C04; EM; 3.27 A; E=2-110.
DR PDB; 6C05; EM; 5.15 A; E=2-110.
DR PDB; 6C06; EM; 5.15 A; E=2-110.
DR PDB; 6DV9; X-ray; 3.80 A; E=1-110.
DR PDB; 6DVB; X-ray; 3.80 A; E=1-110.
DR PDB; 6DVC; X-ray; 3.30 A; E=1-110.
DR PDB; 6DVD; X-ray; 3.90 A; E=1-110.
DR PDB; 6DVE; X-ray; 3.81 A; E=1-110.
DR PDB; 6EDT; EM; -; E=2-110.
DR PDB; 6EE8; EM; 3.92 A; E=2-110.
DR PDB; 6EEC; EM; 3.55 A; E=2-110.
DR PDB; 6FBV; EM; 3.50 A; E=1-110.
DR PDB; 6JCX; X-ray; 2.90 A; E=1-110.
DR PDB; 6JCY; X-ray; 3.11 A; E=1-110.
DR PDB; 6KON; X-ray; 3.00 A; E=1-110.
DR PDB; 6KOO; X-ray; 2.80 A; E=1-110.
DR PDB; 6KOP; X-ray; 3.30 A; E=1-110.
DR PDB; 6KOQ; X-ray; 3.35 A; E=1-110.
DR PDB; 6M7J; EM; 4.40 A; E=2-110.
DR PDB; 6TYE; X-ray; 3.79 A; E=1-110.
DR PDB; 6TYF; X-ray; 3.80 A; E=1-110.
DR PDB; 6TYG; X-ray; 3.50 A; E=1-110.
DR PDB; 6VVX; EM; 3.39 A; E=2-110.
DR PDB; 6VVY; EM; 3.42 A; E=2-110.
DR PDB; 6VVZ; EM; 3.72 A; E=2-110.
DR PDB; 6VW0; EM; 3.59 A; E=2-110.
DR PDB; 7KIF; EM; 2.94 A; E=1-110.
DR PDB; 7KIM; EM; 3.38 A; E=1-110.
DR PDB; 7KIN; EM; 2.74 A; E=1-110.
DR PDBsum; 5UH5; -.
DR PDBsum; 5UH6; -.
DR PDBsum; 5UH8; -.
DR PDBsum; 5UH9; -.
DR PDBsum; 5UHA; -.
DR PDBsum; 5UHB; -.
DR PDBsum; 5UHC; -.
DR PDBsum; 5UHD; -.
DR PDBsum; 5UHE; -.
DR PDBsum; 5UHF; -.
DR PDBsum; 5UHG; -.
DR PDBsum; 5ZX2; -.
DR PDBsum; 5ZX3; -.
DR PDBsum; 6BZO; -.
DR PDBsum; 6C04; -.
DR PDBsum; 6C05; -.
DR PDBsum; 6C06; -.
DR PDBsum; 6DV9; -.
DR PDBsum; 6DVB; -.
DR PDBsum; 6DVC; -.
DR PDBsum; 6DVD; -.
DR PDBsum; 6DVE; -.
DR PDBsum; 6EDT; -.
DR PDBsum; 6EE8; -.
DR PDBsum; 6EEC; -.
DR PDBsum; 6FBV; -.
DR PDBsum; 6JCX; -.
DR PDBsum; 6JCY; -.
DR PDBsum; 6KON; -.
DR PDBsum; 6KOO; -.
DR PDBsum; 6KOP; -.
DR PDBsum; 6KOQ; -.
DR PDBsum; 6M7J; -.
DR PDBsum; 6TYE; -.
DR PDBsum; 6TYF; -.
DR PDBsum; 6TYG; -.
DR PDBsum; 6VVX; -.
DR PDBsum; 6VVY; -.
DR PDBsum; 6VVZ; -.
DR PDBsum; 6VW0; -.
DR PDBsum; 7KIF; -.
DR PDBsum; 7KIM; -.
DR PDBsum; 7KIN; -.
DR AlphaFoldDB; P9WGY5; -.
DR SASBDB; P9WGY5; -.
DR SMR; P9WGY5; -.
DR IntAct; P9WGY5; 3.
DR STRING; 83332.Rv1390; -.
DR PaxDb; P9WGY5; -.
DR DNASU; 886754; -.
DR GeneID; 45425368; -.
DR GeneID; 886754; -.
DR KEGG; mtu:Rv1390; -.
DR TubercuList; Rv1390; -.
DR eggNOG; COG1758; Bacteria.
DR OMA; QHADSKY; -.
DR PhylomeDB; P9WGY5; -.
DR BRENDA; 2.7.7.6; 3445.
DR Reactome; R-HSA-9639775; Antimicrobial action and antimicrobial resistance in Mtb.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.940.10; -; 1.
DR HAMAP; MF_00366; RNApol_bact_RpoZ; 1.
DR InterPro; IPR003716; DNA-dir_RNA_pol_omega.
DR InterPro; IPR006110; Pol_omega/Rpo6/RPB6.
DR InterPro; IPR036161; RPB6/omega-like_sf.
DR PANTHER; PTHR34476; PTHR34476; 1.
DR Pfam; PF01192; RNA_pol_Rpb6; 1.
DR SMART; SM01409; RNA_pol_Rpb6; 1.
DR SUPFAM; SSF63562; SSF63562; 1.
DR TIGRFAMs; TIGR00690; rpoZ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..110
FT /note="DNA-directed RNA polymerase subunit omega"
FT /id="PRO_0000128952"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:7KIN"
FT HELIX 40..43
FT /evidence="ECO:0007829|PDB:7KIN"
FT STRAND 46..49
FT /evidence="ECO:0007829|PDB:7KIN"
FT HELIX 50..69
FT /evidence="ECO:0007829|PDB:7KIN"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:7KIN"
FT HELIX 91..100
FT /evidence="ECO:0007829|PDB:7KIN"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:7KIN"
SQ SEQUENCE 110 AA; 11842 MW; ED2FBEB38F46FF0F CRC64;
MSISQSDASL AAVPAVDQFD PSSGASGGYD TPLGITNPPI DELLDRVSSK YALVIYAAKR
ARQINDYYNQ LGEGILEYVG PLVEPGLQEK PLSIALREIH ADLLEHTEGE
