AUG2_ARATH
ID AUG2_ARATH Reviewed; 296 AA.
AC O48767; Q8LAL1; Q943Z8;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=AUGMIN subunit 2 {ECO:0000303|PubMed:22505726};
GN Name=AUG2 {ECO:0000303|PubMed:22505726};
GN OrderedLocusNames=At2g32980 {ECO:0000312|Araport:AT2G32980};
GN ORFNames=T21L14.8 {ECO:0000312|EMBL:AAL27508.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, IDENTIFICATION IN THE AUGMIN COMPLEX BY MASS SPECTROMETRY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=22505726; DOI=10.1105/tpc.112.096610;
RA Hotta T., Kong Z., Ho C.M., Zeng C.J., Horio T., Fong S., Vuong T.,
RA Lee Y.R., Liu B.;
RT "Characterization of the Arabidopsis augmin complex uncovers its critical
RT function in the assembly of the acentrosomal spindle and phragmoplast
RT microtubule arrays.";
RL Plant Cell 24:1494-1509(2012).
CC -!- FUNCTION: Contributes to the assembly of the acentrosomal spindle and
CC phragmoplast microtubule arrays as part of the augmin complex.
CC {ECO:0000269|PubMed:22505726}.
CC -!- SUBUNIT: Part of the augmin complex composed of 8 subunits. The complex
CC acts on microtubules and interacts with gamma-tubulin in spindles and
CC the phragmoplast. {ECO:0000269|PubMed:22505726}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous.
CC {ECO:0000269|PubMed:22505726}.
CC -!- SIMILARITY: Belongs to the HAUS2 family. {ECO:0000305}.
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DR EMBL; AC003033; AAB91972.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08770.1; -; Genomic_DNA.
DR EMBL; AF439838; AAL27508.1; -; mRNA.
DR EMBL; AY125566; AAM78076.1; -; mRNA.
DR EMBL; AY087746; AAM65283.1; -; mRNA.
DR PIR; T01109; T01109.
DR RefSeq; NP_565760.1; NM_128858.4.
DR AlphaFoldDB; O48767; -.
DR IntAct; O48767; 4.
DR STRING; 3702.AT2G32980.1; -.
DR iPTMnet; O48767; -.
DR PaxDb; O48767; -.
DR PRIDE; O48767; -.
DR ProteomicsDB; 240927; -.
DR EnsemblPlants; AT2G32980.1; AT2G32980.1; AT2G32980.
DR GeneID; 817860; -.
DR Gramene; AT2G32980.1; AT2G32980.1; AT2G32980.
DR KEGG; ath:AT2G32980; -.
DR Araport; AT2G32980; -.
DR TAIR; locus:2059390; AT2G32980.
DR eggNOG; ENOG502QRF0; Eukaryota.
DR HOGENOM; CLU_063148_0_0_1; -.
DR InParanoid; O48767; -.
DR OMA; WVGRKPL; -.
DR OrthoDB; 1051466at2759; -.
DR PhylomeDB; O48767; -.
DR PRO; PR:O48767; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48767; baseline and differential.
DR Genevisible; O48767; AT.
DR GO; GO:1990498; C:mitotic spindle microtubule; IBA:GO_Central.
DR GO; GO:0000911; P:cytokinesis by cell plate formation; IMP:TAIR.
DR GO; GO:0007020; P:microtubule nucleation; IMP:TAIR.
DR GO; GO:0031023; P:microtubule organizing center organization; IEA:InterPro.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:TAIR.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR InterPro; IPR028346; HAUS2.
DR PANTHER; PTHR16039; PTHR16039; 1.
DR Pfam; PF15003; HAUS2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..296
FT /note="AUGMIN subunit 2"
FT /id="PRO_0000434092"
FT REGION 218..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 56..83
FT /evidence="ECO:0000255"
FT COILED 253..285
FT /evidence="ECO:0000255"
FT COMPBIAS 249..264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 265
FT /note="N -> NE (in Ref. 4; AAM65283)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 296 AA; 33019 MW; F474C52225D49CB1 CRC64;
MSMGGDTTWV GKKPIRRIGG LSDALSIASD LGFAVAPPPS QEELQSFASS NGERGDDLIR
VLRELSVVQR KIADLQVELQ GRKDDKNVAH LTHVGEMQKK IETLSRITQI LKDVIQNKDR
IIARLQQPYS LDCIPVEAEY QKQFSELLMK AASDYGALTA SVSDFQWSQN FKEPPSVWGE
MLRPIPVALA SCTRFFEAMS AMRESFATLQ ELRVGNSAVS LPTTPGGNEM THRDSDCVTP
PQGRIESSFD DLAVHKTRRQ NNDQNEEEEE EEEEEDGNNN GNRRLSWPPS VKKSSV
