AUG4_ARATH
ID AUG4_ARATH Reviewed; 423 AA.
AC Q8GYM3; Q9C6P2; Q9C6T4;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=AUGMIN subunit 4 {ECO:0000303|PubMed:22505726};
GN Name=AUG4; OrderedLocusNames=At1g50710 {ECO:0000312|Araport:AT1G50710};
GN ORFNames=F17J6.23 {ECO:0000312|EMBL:AAG51195.1},
GN F4M15.6 {ECO:0000312|EMBL:AAG50783.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAC42179.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, IDENTIFICATION IN THE AUGMIN COMPLEX BY MASS SPECTROMETRY,
RP DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=22505726; DOI=10.1105/tpc.112.096610;
RA Hotta T., Kong Z., Ho C.M., Zeng C.J., Horio T., Fong S., Vuong T.,
RA Lee Y.R., Liu B.;
RT "Characterization of the Arabidopsis augmin complex uncovers its critical
RT function in the assembly of the acentrosomal spindle and phragmoplast
RT microtubule arrays.";
RL Plant Cell 24:1494-1509(2012).
CC -!- FUNCTION: Involved in microtubules reorganization during spindle and
CC phragmoplast development. {ECO:0000269|PubMed:22505726}.
CC -!- SUBUNIT: Part of the augmin complex composed of 8 subunits. The complex
CC acts on microtubules and interacts with gamma-tubulin in spindles and
CC the phragmoplast. {ECO:0000269|PubMed:22505726}.
CC -!- INTERACTION:
CC Q8GYM3; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-25520241, EBI-4426557;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:22505726}. Cytoplasm, cytoskeleton, phragmoplast
CC {ECO:0000269|PubMed:22505726}. Note=Preferentially localizes to
CC microtubules minus ends. {ECO:0000269|PubMed:22505726}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous.
CC {ECO:0000269|PubMed:22505726}.
CC -!- SIMILARITY: Belongs to the HAUS4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51195.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC079027; AAG50783.1; -; Genomic_DNA.
DR EMBL; AC079279; AAG51195.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32582.1; -; Genomic_DNA.
DR EMBL; AK117516; BAC42179.1; -; mRNA.
DR EMBL; BT005509; AAO63929.1; -; mRNA.
DR PIR; H96543; H96543.
DR RefSeq; NP_175486.2; NM_103953.3.
DR AlphaFoldDB; Q8GYM3; -.
DR SMR; Q8GYM3; -.
DR IntAct; Q8GYM3; 1.
DR STRING; 3702.AT1G50710.1; -.
DR PaxDb; Q8GYM3; -.
DR PRIDE; Q8GYM3; -.
DR ProteomicsDB; 240929; -.
DR EnsemblPlants; AT1G50710.1; AT1G50710.1; AT1G50710.
DR GeneID; 841493; -.
DR Gramene; AT1G50710.1; AT1G50710.1; AT1G50710.
DR KEGG; ath:AT1G50710; -.
DR Araport; AT1G50710; -.
DR TAIR; locus:2015857; AT1G50710.
DR eggNOG; ENOG502QQ47; Eukaryota.
DR HOGENOM; CLU_036252_0_0_1; -.
DR InParanoid; Q8GYM3; -.
DR OMA; RCQTMNA; -.
DR OrthoDB; 978649at2759; -.
DR PhylomeDB; Q8GYM3; -.
DR PRO; PR:Q8GYM3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GYM3; baseline and differential.
DR Genevisible; Q8GYM3; AT.
DR GO; GO:0070652; C:HAUS complex; IBA:GO_Central.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005876; C:spindle microtubule; IDA:TAIR.
DR GO; GO:0051011; F:microtubule minus-end binding; IDA:TAIR.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR InterPro; IPR029327; HAUS4.
DR PANTHER; PTHR16219; PTHR16219; 1.
DR Pfam; PF14735; HAUS4; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..423
FT /note="AUGMIN subunit 4"
FT /id="PRO_0000434094"
FT COILED 267..287
FT /evidence="ECO:0000255"
SQ SEQUENCE 423 AA; 47770 MW; 6162027D129A8EDC CRC64;
MVKALQGAAQ NLPADVNQLI DQLERHCLAP DGSLVTKSVY SDLQLAREEM SRERLRYLEA
MAIYCEAVAM VEEYQQAISV ANHGGIRDVQ GLYPQLGLKN SPQVYETLEH RLVVAEAAQK
LRLPLISDGG EIHEEEIEKW SILSRSSLDS ASTSFTISST SNSVNYANSS ANSVAGGISL
SAVDTDVVGG VPNRFLGITP AYLSYVQLQN TISMDMADYQ MFLAREIEGR LKEKCDKLAD
AIVDDTDSST GNRNSSARLP ERVKFIIEEI ERDEAALRED LYSADRKFAE YYNVLEQILG
VLIKLVKDLK LEHQHKYNEM QKTWLCKRCE TMNAKLRVLE NVLLLETYTP DSISALHNIR
NYLVEATEEA SAAYNKAVTR LREYQGVDPH FDTIARQYHD IVKKLENMQW TIHQVEMDLK
SHD
