AUGNA_DANRE
ID AUGNA_DANRE Reviewed; 142 AA.
AC Q566V9; F1R4S5;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2019, sequence version 2.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Augurin-A;
DE AltName: Full=Esophageal cancer-related gene 4-A protein homolog {ECO:0000312|ZFIN:ZDB-GENE-050417-189};
DE Flags: Precursor;
GN Name=ecrg4a {ECO:0000312|ZFIN:ZDB-GENE-050417-189}; ORFNames=zgc:112443;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21349154; DOI=10.1186/2045-8118-8-6;
RA Gonzalez A.M., Podvin S., Lin S.Y., Miller M.C., Botfield H.,
RA Leadbeater W.E., Roberton A., Dang X., Knowling S.E., Cardenas-Galindo E.,
RA Donahue J.E., Stopa E.G., Johanson C.E., Coimbra R., Eliceiri B.P.,
RA Baird A.;
RT "Ecrg4 expression and its product augurin in the choroid plexus: impact on
RT fetal brain development, cerebrospinal fluid homeostasis and
RT neuroprogenitor cell response to CNS injury.";
RL Fluids Barriers CNS 8:6-6(2011).
CC -!- FUNCTION: Probable hormone (By similarity). Required for the proper
CC formation of the central nervous system by attenuating cell
CC proliferation during development (PubMed:21349154).
CC {ECO:0000250|UniProtKB:D4A540, ECO:0000269|PubMed:21349154}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9H1Z8}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q9H1Z8}. Apical cell membrane
CC {ECO:0000250|UniProtKB:Q9H1Z8}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown causes severe defects in the
CC development of the central nervous system, including enlarged hindbrain
CC ventricles resembling a brain ventricular hydrocephalus-like edema
CC phenotype (PubMed:21349154). Increased cell proliferation of glial
CC fibrillary acidic protein (GFAP)-positive cells in the developing
CC central nervous system (PubMed:21349154).
CC {ECO:0000269|PubMed:21349154}.
CC -!- SIMILARITY: Belongs to the augurin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CABZ01042254; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CABZ01089983; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU639407; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC093311; AAH93311.1; -; mRNA.
DR RefSeq; NP_001017697.1; NM_001017697.1.
DR AlphaFoldDB; Q566V9; -.
DR STRING; 7955.ENSDARP00000072984; -.
DR PaxDb; Q566V9; -.
DR Ensembl; ENSDART00000078523; ENSDARP00000072984; ENSDARG00000056087.
DR GeneID; 791762; -.
DR KEGG; dre:791762; -.
DR CTD; 791762; -.
DR ZFIN; ZDB-GENE-050417-189; ecrg4a.
DR eggNOG; ENOG502RZPP; Eukaryota.
DR GeneTree; ENSGT00390000000145; -.
DR HOGENOM; CLU_153579_0_0_1; -.
DR InParanoid; Q566V9; -.
DR OMA; RNGHDYD; -.
DR OrthoDB; 1431738at2759; -.
DR PhylomeDB; Q566V9; -.
DR TreeFam; TF336161; -.
DR PRO; PR:Q566V9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 9.
DR Bgee; ENSDARG00000056087; Expressed in bone element and 15 other tissues.
DR ExpressionAtlas; Q566V9; baseline.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0090398; P:cellular senescence; IBA:GO_Central.
DR GO; GO:0007417; P:central nervous system development; IMP:ZFIN.
DR GO; GO:0070314; P:G1 to G0 transition; IBA:GO_Central.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:ZFIN.
DR GO; GO:0009611; P:response to wounding; ISS:UniProtKB.
DR InterPro; IPR028173; Augurin.
DR PANTHER; PTHR31613; PTHR31613; 1.
DR Pfam; PF15187; Augurin; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cleavage on pair of basic residues; Cytoplasm; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT PROPEP 29..66
FT /evidence="ECO:0000250|UniProtKB:D4A540"
FT /id="PRO_0000446067"
FT PEPTIDE 69..126
FT /note="Augurin-A"
FT /id="PRO_0000359772"
FT PROPEP 127..142
FT /evidence="ECO:0000255"
FT /id="PRO_0000363242"
FT CONFLICT 9
FT /note="R -> H (in Ref. 2; AAH93311)"
FT /evidence="ECO:0000305"
FT CONFLICT 21
FT /note="S -> A (in Ref. 2; AAH93311)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 142 AA; 16488 MW; 7D5B291D134B7723 CRC64;
MLSEKFHLRL LTLLTLLTAL SLTDVASESK LEKLLMKRVD RDVKPAAAVA VSPSKAKEFL
TSLKRPKRNL WDRSRPDVQQ WIQQFMYMGF DEARLETDLA YWMDQHRSSD QGRQHHYDEN
AALGPRGAAS YRHGANVNYD YY
