AUGN_RAT
ID AUGN_RAT Reviewed; 148 AA.
AC D4A540;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Augurin {ECO:0000305};
DE AltName: Full=Esophageal cancer-related gene 4 protein homolog;
DE Flags: Precursor;
GN Name=Ecrg4 {ECO:0000305};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP FUNCTION.
RX PubMed=20404145; DOI=10.1073/pnas.0911446107;
RA Kujuro Y., Suzuki N., Kondo T.;
RT "Esophageal cancer-related gene 4 is a secreted inducer of cell senescence
RT expressed by aged CNS precursor cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:8259-8264(2010).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21349154; DOI=10.1186/2045-8118-8-6;
RA Gonzalez A.M., Podvin S., Lin S.Y., Miller M.C., Botfield H.,
RA Leadbeater W.E., Roberton A., Dang X., Knowling S.E., Cardenas-Galindo E.,
RA Donahue J.E., Stopa E.G., Johanson C.E., Coimbra R., Eliceiri B.P.,
RA Baird A.;
RT "Ecrg4 expression and its product augurin in the choroid plexus: impact on
RT fetal brain development, cerebrospinal fluid homeostasis and
RT neuroprogenitor cell response to CNS injury.";
RL Fluids Barriers CNS 8:6-6(2011).
RN [4]
RP CLEAVAGE OF SIGNAL PEPTIDE AFTER GLY-31, CLEAVAGE OF PROPEPTIDE AFTER
RP ALA-68, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=26479776; DOI=10.1021/acs.jproteome.5b00820;
RA Tsuchiya T., Osaki T., Minamino N., Sasaki K.;
RT "Peptidomics for studying limited proteolysis.";
RL J. Proteome Res. 14:4921-4931(2015).
CC -!- FUNCTION: Probable hormone that may attenuate cell proliferation and
CC induce senescence of oligodendrocyte and neural precursor cells in the
CC central nervous system (PubMed:20404145, PubMed:21349154). ECRG4-
CC induced senescence is characterized by G1 arrest, RB1 dephosphorylation
CC and accelerated CCND1 and CCND3 proteasomal degradation
CC (PubMed:20404145). {ECO:0000269|PubMed:20404145,
CC ECO:0000269|PubMed:21349154}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9H1Z8}.
CC Cytoplasm {ECO:0000269|PubMed:21349154}. Apical cell membrane
CC {ECO:0000250|UniProtKB:Q9H1Z8}.
CC -!- TISSUE SPECIFICITY: Expressed in the brain, with expression in the
CC choroid plexus and the ventricular ependymal cells (at protein level).
CC {ECO:0000269|PubMed:21349154}.
CC -!- INDUCTION: Decreased expression in the choroid plexus after penetrating
CC injury in the central nervous system. {ECO:0000269|PubMed:21349154}.
CC -!- SIMILARITY: Belongs to the augurin family. {ECO:0000305}.
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DR RefSeq; NP_001257980.1; NM_001271051.1.
DR AlphaFoldDB; D4A540; -.
DR STRING; 10116.ENSRNOP00000030526; -.
DR iPTMnet; D4A540; -.
DR PhosphoSitePlus; D4A540; -.
DR PaxDb; D4A540; -.
DR Ensembl; ENSRNOT00000036990; ENSRNOP00000030526; ENSRNOG00000023576.
DR GeneID; 363225; -.
DR KEGG; rno:363225; -.
DR UCSC; RGD:1305645; rat.
DR CTD; 84417; -.
DR RGD; 1305645; RGD1305645.
DR eggNOG; ENOG502RZPP; Eukaryota.
DR GeneTree; ENSGT00390000000145; -.
DR HOGENOM; CLU_153579_0_0_1; -.
DR InParanoid; D4A540; -.
DR OMA; RNGHDYD; -.
DR OrthoDB; 1431738at2759; -.
DR PhylomeDB; D4A540; -.
DR TreeFam; TF336161; -.
DR PRO; PR:D4A540; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000023576; Expressed in kidney and 19 other tissues.
DR Genevisible; D4A540; RN.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031045; C:dense core granule; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; ISO:RGD.
DR GO; GO:0090398; P:cellular senescence; ISO:RGD.
DR GO; GO:0007417; P:central nervous system development; ISS:UniProtKB.
DR GO; GO:0070314; P:G1 to G0 transition; ISO:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0009611; P:response to wounding; IMP:UniProtKB.
DR InterPro; IPR028173; Augurin.
DR PANTHER; PTHR31613; PTHR31613; 1.
DR Pfam; PF15187; Augurin; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cleavage on pair of basic residues; Cytoplasm; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000269|PubMed:26479776"
FT PROPEP 32..68
FT /evidence="ECO:0000269|PubMed:26479776"
FT /id="PRO_0000415563"
FT PEPTIDE 71..132
FT /note="Augurin"
FT /id="PRO_0000415564"
FT PROPEP 133..148
FT /evidence="ECO:0000255"
FT /id="PRO_0000415565"
SQ SEQUENCE 148 AA; 16949 MW; 641F8358E067BF60 CRC64;
MGTSSARPAV LALAGLALLL LLCLGPGDVS GNKLKKMLQK REGPVPSKTN VAVSEHTAKE
FLGGLKRAKR QLWDRTRPEV QQWYQQFLYM GFDEAKFEDD VNYWLNRNQN GHDYYGDYYQ
RHYDEDAAIG PRSREGFRHG ASVNYDDY
