RPP13_ARATH
ID RPP13_ARATH Reviewed; 835 AA.
AC Q9M667; Q9M668; Q9SNC5;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Disease resistance protein RPP13;
DE AltName: Full=Resistance to Peronospora parasitica protein 13;
GN Name=RPP13; OrderedLocusNames=At3g46530; ORFNames=F12A12.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND VARIANTS.
RC STRAIN=cv. Columbia, cv. Nd-1, and cv. RLD;
RX PubMed=10743658; DOI=10.1046/j.1365-313x.2000.00664.x;
RA Bittner-Eddy P.D., Crute I.R., Holub E.B., Beynon J.L.;
RT "RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify
RT downy mildew resistance to different avirulence determinants in Peronospora
RT parasitica.";
RL Plant J. 21:177-188(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX PubMed=11277440; DOI=10.1094/mpmi.2001.14.3.416;
RA Bittner-Eddy P.D., Beynon J.L.;
RT "The Arabidopsis downy mildew resistance gene, RPP13-Nd, functions
RT independently of NDR1 and EDS1 and does not require the accumulation of
RT salicylic acid.";
RL Mol. Plant Microbe Interact. 14:416-421(2001).
CC -!- FUNCTION: Disease resistance protein. Resistance proteins guard the
CC plant against pathogens that contain an appropriate avirulence protein
CC via an indirect interaction with this avirulence protein. That triggers
CC a defense system including the hypersensitive response, which restricts
CC the pathogen growth. In contrast to other resistance proteins, it works
CC independently of ESD1 and NSD1 proteins and does not require the
CC accumulation of salicylic acid, suggesting the existence of an
CC independent signaling pathway. The specificity to avirulence proteins
CC differs in the different cultivars. {ECO:0000269|PubMed:10743658,
CC ECO:0000269|PubMed:11277440}.
CC -!- DOMAIN: The LRR repeats probably act as specificity determinant of
CC pathogen recognition. {ECO:0000250}.
CC -!- MISCELLANEOUS: In cv. Nd-1, it confers resistance to Mask-9, Aswa1,
CC Edco1, Emco5 and Goco5 loci from Hyaloperonospora parasitica. In cv.
CC Columbia, it confers resistance to Wela3 locus from Hyaloperonospora
CC parasitica. In cv. RLD, it confers resistance to all of these loci.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family. RPP13
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AF209730; AAF42830.1; -; Genomic_DNA.
DR EMBL; AF209731; AAF42831.1; -; Genomic_DNA.
DR EMBL; AF209732; AAF42832.1; -; Genomic_DNA.
DR EMBL; AL133314; CAB62323.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78169.1; -; Genomic_DNA.
DR EMBL; AY037179; AAK59764.1; -; mRNA.
DR EMBL; BT002246; AAN72257.1; -; mRNA.
DR PIR; T45590; T45590.
DR PIR; T51185; T51185.
DR PIR; T51186; T51186.
DR RefSeq; NP_190237.1; NM_114520.3.
DR AlphaFoldDB; Q9M667; -.
DR SMR; Q9M667; -.
DR BioGRID; 9126; 1.
DR STRING; 3702.AT3G46530.1; -.
DR iPTMnet; Q9M667; -.
DR PaxDb; Q9M667; -.
DR PRIDE; Q9M667; -.
DR ProteomicsDB; 228078; -.
DR EnsemblPlants; AT3G46530.1; AT3G46530.1; AT3G46530.
DR GeneID; 823806; -.
DR Gramene; AT3G46530.1; AT3G46530.1; AT3G46530.
DR KEGG; ath:AT3G46530; -.
DR Araport; AT3G46530; -.
DR TAIR; locus:2075170; AT3G46530.
DR eggNOG; KOG4658; Eukaryota.
DR HOGENOM; CLU_000837_25_4_1; -.
DR InParanoid; Q9M667; -.
DR OMA; VKSEFRH; -.
DR OrthoDB; 463722at2759; -.
DR PhylomeDB; Q9M667; -.
DR PRO; PR:Q9M667; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M667; baseline and differential.
DR Genevisible; Q9M667; AT.
DR GO; GO:0005737; C:cytoplasm; TAS:TAIR.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0098542; P:defense response to other organism; IMP:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:TAIR.
DR CDD; cd14798; RX-CC_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038005; RX-like_CC.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Coiled coil; Hypersensitive response; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..835
FT /note="Disease resistance protein RPP13"
FT /id="PRO_0000212725"
FT DOMAIN 144..453
FT /note="NB-ARC"
FT COILED 25..41
FT /evidence="ECO:0000255"
FT BINDING 192..199
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VARIANT 108
FT /note="I -> V (in strain: cv. Nd-1)"
FT VARIANT 113
FT /note="R -> K (in strain: cv. Nd-1)"
FT VARIANT 167
FT /note="E -> A (in strain: cv. Nd-1)"
FT VARIANT 181
FT /note="E -> D (in strain: cv. Nd-1)"
FT VARIANT 209
FT /note="R -> G (in strain: cv. Nd-1)"
FT VARIANT 213
FT /note="E -> R (in strain: cv. Nd-1)"
FT VARIANT 238
FT /note="R -> K (in strain: cv. Nd-1)"
FT VARIANT 253
FT /note="K -> M (in strain: cv. Nd-1)"
FT VARIANT 256
FT /note="E -> D (in strain: cv. RLD)"
FT VARIANT 264
FT /note="Y -> H (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 286
FT /note="D -> E (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 314
FT /note="V -> L (in strain: cv. Nd-1)"
FT VARIANT 346
FT /note="K -> N (in strain: cv. RLD)"
FT VARIANT 435
FT /note="L -> V (in strain: cv. RLD)"
FT VARIANT 440
FT /note="H -> R (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 514
FT /note="A -> S (in strain: cv. Nd-1)"
FT VARIANT 524
FT /note="E -> Q (in strain: cv. Nd-1)"
FT VARIANT 527
FT /note="H -> N (in strain: cv. Nd-1)"
FT VARIANT 530..539
FT /note="FKRYSSEKRK -> IKRFAR (in strain: cv. Nd-1)"
FT VARIANT 530
FT /note="F -> V (in strain: cv. RLD)"
FT VARIANT 548
FT /note="Y -> N (in strain: cv. RLD)"
FT VARIANT 551..554
FT /note="EFDH -> LDN (in strain: cv. RLD)"
FT VARIANT 552..562
FT /note="FDHLVGLDFET -> LKFLVRLDVEK (in strain: cv. Nd-1)"
FT VARIANT 558
FT /note="L -> P (in strain: cv. RLD)"
FT VARIANT 563
FT /note="L -> T (in strain: cv. RLD)"
FT VARIANT 571..580
FT /note="FGSLWLPFKI -> LEALLGPFAS (in strain: cv. Nd-1)"
FT VARIANT 571..578
FT /note="FGSLWLPF -> VRRLGFPL (in strain: cv. RLD)"
FT VARIANT 586
FT /note="H -> P (in strain: cv. RLD)"
FT VARIANT 591..592
FT /note="GI -> RF (in strain: cv. Nd-1)"
FT VARIANT 594..602
FT /note="GNSINDFDI -> DYSFSDR (in strain: cv. RLD)"
FT VARIANT 595..599
FT /note="NSIND -> FSLRV (in strain: cv. Nd-1)"
FT VARIANT 609
FT /note="L -> S (in strain: cv. Nd-1)"
FT VARIANT 616..625
FT /note="FVSDNYFIEE -> DADHVCFIYD (in strain: cv. Nd-1)"
FT VARIANT 616..625
FT /note="FVSDNYFIEE -> EVSTYSEYPIYD (in strain: cv. RLD)"
FT VARIANT 632
FT /note="L -> F (in strain: cv. Nd-1)"
FT VARIANT 641
FT /note="N -> K (in strain: cv. Nd-1)"
FT VARIANT 641
FT /note="N -> Q (in strain: cv. RLD)"
FT VARIANT 643..645
FT /note="FGG -> VGE (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 651
FT /note="V -> A (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 658
FT /note="T -> R (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 659
FT /note="S -> F (in strain: cv. RLD)"
FT VARIANT 662
FT /note="F -> S (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 666
FT /note="N -> S (in strain: cv. Nd-1)"
FT VARIANT 670
FT /note="P -> H (in strain: cv. Nd-1)"
FT VARIANT 680..684
FT /note="GISEM -> EIYEDY (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 685..689
FT /note="SRSKE -> DEDFD (in strain: cv. RLD)"
FT VARIANT 685..686
FT /note="SR -> NK (in strain: cv. Nd-1)"
FT VARIANT 693
FT /note="H -> T (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 703
FT /note="E -> R (in strain: cv. Nd-1 and cv. RLD)"
FT VARIANT 711..719
FT /note="ATPTEVHLS -> YYLR (in strain: cv. RLD)"
FT VARIANT 711..717
FT /note="ATPTEVH -> VADRRY (in strain: cv. Nd-1)"
FT VARIANT 729
FT /note="M -> T (in strain: cv. RLD)"
FT VARIANT 734
FT /note="R -> L (in strain: cv. RLD)"
FT VARIANT 734
FT /note="R -> P (in strain: cv. Nd-1)"
FT VARIANT 742
FT /note="V -> E (in strain: cv. RLD)"
FT VARIANT 750
FT /note="P -> T (in strain: cv. RLD)"
FT VARIANT 765..771
FT /note="LLSCNYS -> FENCDYWG (in strain: cv. Nd-1)"
FT VARIANT 767
FT /note="S -> H (in strain: cv. RLD)"
FT VARIANT 772
FT /note="G -> GG (in strain: cv. RLD)"
FT VARIANT 788..793
FT /note="DLLMRS -> KLFINR (in strain: cv. Nd-1)"
FT VARIANT 788..792
FT /note="DLLMR -> QIFIHN (in strain: cv. RLD)"
FT VARIANT 811..823
FT /note="EISVSKRETKLII -> VVQTDNSKPIASV (in strain: cv. Nd-
FT 1)"
FT VARIANT 811..819
FT /note="EISVSKRET -> KITFKEVK (in strain: cv. RLD)"
FT VARIANT 830..835
FT /note="FGQIYC -> CMSYES (in strain: cv. RLD)"
SQ SEQUENCE 835 AA; 97267 MW; 2B8DCB7102F3C2E3 CRC64;
MVDAITEFVV GKIGNYLIEE ASMFMAVKED LEELKTELTC IHGYLKDVEA REREDEVSKE
WSKLVLDFAY DVEDVLDTYH LKLEERSQRR GLRRLTNKIG RKMDAYSIVD DIRILKRRIL
DITRKRETYG IGGLKEPQGG GNTSSLRVRQ LRRARSVDQE EVVVGLEDDA KILLEKLLDY
EEKNRFIISI FGMGGLGKTA LARKLYNSRD VKERFEYRAW TYVSQEYKTG DILMRIIRSL
GMTSGEELEK IRKFAEEELE VYLYGLLEGK KYLVVVDDIW EREAWDSLKR ALPCNHEGSR
VIITTRIKAV AEGVDGRFYA HKLRFLTFEE SWELFEQRAF RNIQRKDEDL LKTGKEMVQK
CRGLPLCIVV LAGLLSRKTP SEWNDVCNSL WRRLKDDSIH VAPIVFDLSF KELRHESKLC
FLYLSIFPED YEIDLEKLIH LLVAEGFIQG DEEMMMEDVA RYYIEELIDR SLLEAVRRER
GKVMSCRIHD LLRDVAIKKS KELNFVNVYN DHVAQHSSTT CRREVVHHQF KRYSSEKRKN
KRMRSFLYFG EFDHLVGLDF ETLKLLRVLD FGSLWLPFKI NGDLIHLRYL GIDGNSINDF
DIAAIISKLR FLQTLFVSDN YFIEETIDLR KLTSLRHVIG NFFGGLLIGD VANLQTLTSI
SFDSWNKLKP ELLINLRDLG ISEMSRSKER RVHVSWASLT KLESLRVLKL ATPTEVHLSL
ESEEAVRSMD VISRSLESVT LVGITFEEDP MPFLQKMPRL EDLILLSCNY SGKMSVSEQG
FGRLRKLDLL MRSLDELQIE EEAMPNLIEL EISVSKRETK LIIPNRLRAF GQIYC
