ID   AUHM_DICDI              Reviewed;         303 AA.
AC   Q54HG7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Methylglutaconyl-CoA hydratase, mitochondrial;
DE            EC=4.2.1.18;
DE   AltName: Full=AU-specific RNA-binding enoyl-CoA hydratase homolog;
DE            Short=AU-binding protein/enoyl-CoA hydratase homolog;
DE   Flags: Precursor;
GN   Name=auh; ORFNames=DDB_G0289471;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-
CC       hydroxy-3-methylglutaryl-CoA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-hydroxy-3-methylglutaryl-CoA = 3-methyl-(2E)-glutaconyl-
CC         CoA + H2O; Xref=Rhea:RHEA:21536, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:43074, ChEBI:CHEBI:57346; EC=4.2.1.18;
CC   -!- PATHWAY: Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-
CC       3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 3/3.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000141; EAL62715.1; -; Genomic_DNA.
DR   RefSeq; XP_636218.1; XM_631126.1.
DR   AlphaFoldDB; Q54HG7; -.
DR   SMR; Q54HG7; -.
DR   STRING; 44689.DDB0233850; -.
DR   PaxDb; Q54HG7; -.
DR   EnsemblProtists; EAL62715; EAL62715; DDB_G0289471.
DR   GeneID; 8627156; -.
DR   KEGG; ddi:DDB_G0289471; -.
DR   dictyBase; DDB_G0289471; auh.
DR   eggNOG; KOG1679; Eukaryota.
DR   HOGENOM; CLU_009834_7_6_1; -.
DR   InParanoid; Q54HG7; -.
DR   OMA; AMEMIMT; -.
DR   PhylomeDB; Q54HG7; -.
DR   Reactome; R-DDI-70895; Branched-chain amino acid catabolism.
DR   UniPathway; UPA00363; UER00862.
DR   PRO; PR:Q54HG7; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; ISS:UniProtKB.
DR   GO; GO:0004490; F:methylglutaconyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003730; F:mRNA 3'-UTR binding; ISS:UniProtKB.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR   GO; GO:0006552; P:leucine catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE   3: Inferred from homology;
KW   Branched-chain amino acid catabolism; Lyase; Mitochondrion;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..303
FT                   /note="Methylglutaconyl-CoA hydratase, mitochondrial"
FT                   /id="PRO_0000328210"
SQ   SEQUENCE   303 AA;  33245 MW;  A1D47C0A3EBBB2CB CRC64;
     MIKHLIKNVS NSVKYNNVTS NNILFSTSSS LKFGRKFTTE TQQECILERL EGENKGISVI
     SFNRGHVKNA LGKNLMNQFR SHLNELRFCP DTRVVIVRSL VDGVFCSGAD LKERALMSQV
     EASQFVHSLR SSFTELETLQ MPTIAAIEGV AVGGGTEMVL ACDFRVASKS SKMGLPETGL
     AIIPGAGGTQ RLPRLIGIPR AKELIFTGAI LDSKRALEIG LVQYETEKGE AFDKAIEIAK
     QIIPKGPIAI RMAKQAIDRG MNVDQASGMI IEQASYAQVI PTKDRIEGLT AFKEKRKPIY
     KGE