RPP2C_ARATH
ID RPP2C_ARATH Reviewed; 1744 AA.
AC F4JT82; O49470;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Probable disease resistance protein At4g19520 {ECO:0000305|PubMed:15165183};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
GN OrderedLocusNames=At4g19520 {ECO:0000312|Araport:AT4G19520};
GN ORFNames=F24J7.80 {ECO:0000312|EMBL:CAA16929.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=15165183; DOI=10.1111/j.1365-313x.2004.02099.x;
RA Sinapidou E., Williams K., Nott L., Bahkt S., Toer M., Crute I.,
RA Bittner-Eddy P., Beynon J.;
RT "Two TIR:NB:LRR genes are required to specify resistance to Peronospora
RT parasitica isolate Cala2 in Arabidopsis.";
RL Plant J. 38:898-909(2004).
CC -!- FUNCTION: Probable disease resistance protein.
CC {ECO:0000303|PubMed:15165183}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SIMILARITY: Belongs to the disease resistance TIR-NB-LRR family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16929.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78954.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AL021768; CAA16929.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161551; CAB78954.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84194.1; -; Genomic_DNA.
DR PIR; T06145; T06145.
DR RefSeq; NP_193687.3; NM_118072.4.
DR AlphaFoldDB; F4JT82; -.
DR SMR; F4JT82; -.
DR STRING; 3702.AT4G19520.1; -.
DR PaxDb; F4JT82; -.
DR PRIDE; F4JT82; -.
DR ProteomicsDB; 228222; -.
DR EnsemblPlants; AT4G19520.1; AT4G19520.1; AT4G19520.
DR GeneID; 827694; -.
DR Gramene; AT4G19520.1; AT4G19520.1; AT4G19520.
DR KEGG; ath:AT4G19520; -.
DR Araport; AT4G19520; -.
DR TAIR; locus:2122980; AT4G19520.
DR HOGENOM; CLU_001561_0_3_1; -.
DR InParanoid; F4JT82; -.
DR OrthoDB; 49850at2759; -.
DR PRO; PR:F4JT82; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JT82; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0071456; P:cellular response to hypoxia; IEP:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 2.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11017; PTHR11017; 3.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 2.
DR SMART; SM00255; TIR; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 2.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 2.
PE 3: Inferred from homology;
KW ATP-binding; Hydrolase; Leucine-rich repeat; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..1744
FT /note="Probable disease resistance protein At4g19520"
FT /id="PRO_0000444558"
FT DOMAIN 3..163
FT /note="TIR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 192..411
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 503..526
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 557..581
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 583..602
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 603..626
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 648..669
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 670..692
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 710..733
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 734..754
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 755..777
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 779..802
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 804..823
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 824..846
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 848..871
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT REPEAT 892..915
FT /note="LRR 14"
FT /evidence="ECO:0000255"
FT REPEAT 917..939
FT /note="LRR 15"
FT /evidence="ECO:0000255"
FT REPEAT 941..963
FT /note="LRR 16"
FT /evidence="ECO:0000255"
FT REPEAT 987..1010
FT /note="LRR 17"
FT /evidence="ECO:0000255"
FT REPEAT 1011..1035
FT /note="LRR 18"
FT /evidence="ECO:0000255"
FT REPEAT 1037..1059
FT /note="LRR 19"
FT /evidence="ECO:0000255"
FT REPEAT 1062..1086
FT /note="LRR 20"
FT /evidence="ECO:0000255"
FT DOMAIN 1399..1559
FT /note="TIR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT ACT_SITE 80
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
SQ SEQUENCE 1744 AA; 196906 MW; BDEF837606129AFB CRC64;
MVDGKEVYIS FNRWEDTIRH SFVSHLSAEF QRKGVSVFAS EDSASDDRFA EESDAAIAKA
RVSVVIFSEN FASSKGCLNE FLKVSKCRRS KGLVVVPVFY GLTNSIVKKH CLELKKMYPD
DKVDEWRNAL WDIADLRGGH VSSHKRSDSE LVEKIVADVR QKLDRRGRIG VYSRLTKIEY
LLCKQPGCII RSLGIWGMAG IGKTTLARAA YDQLSRDFEA SCFIEDFDRE FQEKGFFGLL
EKQLGVNPQV TRLSILLKTL RSKRILLVLD DVRKPLGATS FLCEFDWLGP GSLIIVTSQD
KQVLVQCQVN EIYKVQGLNK HESLQLFSRC AFGKDVPDQN LLELSMKFVD YANGNPLALS
ICGKNLKGKT PLDMKSVVLE LKRHLSDKIF VKLKSSYDAL SVSEKEIFLD IVFTFRGANV
DNVMQSLAGC GFFPRVGIEA LVDKSFVTVS ENRVQVNNLI YDVGLKIIND QSDEIGMCYR
FVDASNSQSL IEHKEIRESE QGYEDVKAIN LDTSNLPFKG HIAFQHMYNL RYLTIYSSIN
PTKDPDLFLP GDPQFLPPEL RLLHWTCYPL HSFPQNFGFQ YLVELNMPCS KLKKLWGGTK
NLEVLKRITL SCSVQLLNVD ELQYSPNIEK IDLKGCLELQ SFPDTGQLQH LRIVDLSTCK
KIKSFPKVPP SIRKLHLQGT GIRDLSSLNH SSESQRLTRK LENVSSSNQD HRKQVLKLKD
SSHLGSLPDI VIFESLEVLD FSGCSELEDI QGFPQNLKRL YLAKTAIKEV PSSLCHHISK
LVKLDMENCE RLRDLPMGMS NMKYLAVLKL SGCSNLENIK ELPRNLKELY LAGTAVKEFP
STLLETLSEV VLLDLENCKK LQGLPTGMSK LEFLVMLKLS GCSKLEIIVD LPLNLIELYL
AGTAIRELPP SIGDLALLDT LDLKNCNRLR HLPMEMHNLN PLKVLDLSNC SELEVFTSSL
PKVRELRPAP TVMLLRSKLP FCFFIFYEHR VTLSLYKARL QYIPEEIRWM PSLKTLDLSR
NGFTEVPVSI KDFSKLLSLR LRYCENLRSL PQLPRSLQLL NAHGCSSLQL ITPDFKQLPR
YYTFSNCFGL PSHMVSEVLA NAPAIVECRK PQQGLENALA CSFCLPSPTS RDSKLYLQPG
SSTMIILNPK TRSTLVGFAI LVEVSFSKDF HDTAGLGFRC VCRWNDKKGH AHKRDNIFHC
WAPGEVVPKI NDDHMFVFFD LKMHPSILFE GDVFGILADL VVFEIFPVNK QEMHVGDSCT
ITKCGVYVIN DAAGSSSGNT MTPQCSSMDS LKLLDGKGKK RLRVNYAGLK QREKALFLYI
ACLLGGEKAD LLAQFLASTD FVIESTLEDL AGRYLIDISS NGEVMMPPLQ RNFSREIIHM
LPASTKELVS MASGSPCNRN NDVFVSFHGK DFRKQFISDF LKKLVYKGIR ICIGDKILSR
SLINKVIKES SIAVVVFSEN YASSSLCLLQ LMEIMKCWEE LGQVVMPIFY KVNPSDIRNQ
SGHFGKGFKK TCKKTINDER QRWSRALTDA ASIAGECSLN WASDADMIEK VANDIRKKLI
SSKKLGKQIQ RVDCDHDNPW ETEFNKCIER FFQLPYDGNH DESLVSLTTE KVIDMKQTLK
EIYQITKVKL KNNLVGRRHI NNICFMHILY EKCEERSSFN PTSLHTLYDN GIPYQVYTKR
KKHMSGEEKA PIGGCVGCFY MGQNQGITKA GGGRTMPTAY PAAPQANQGY NAGAQPYPPT
AYAV
