RPP4_ARATH
ID RPP4_ARATH Reviewed; 1147 AA.
AC F4JNA9; O23527; O23528; Q8GW40; Q8L843; Q8S4Q0;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Disease resistance protein RPP4 {ECO:0000305};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein CHILLING-SENSITIVE 2 {ECO:0000303|PubMed:20699401};
DE AltName: Full=Protein RECOGNITION OF PERONOSPORA PARASITICA 4 {ECO:0000303|PubMed:11846877};
GN Name=RPP4 {ECO:0000303|PubMed:11846877};
GN Synonyms=CHS2 {ECO:0000303|PubMed:20699401};
GN OrderedLocusNames=At4g16860 {ECO:0000312|Araport:AT4G16860};
GN ORFNames=dl4460c {ECO:0000312|EMBL:CAB10461.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH RSH1.
RX PubMed=11846877; DOI=10.1046/j.0960-7412.2001.01229.x;
RA van der Biezen E.A., Freddie C.T., Kahn K., Parker J.E., Jones J.D.;
RT "Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-LRR
RT genes and confers downy mildew resistance through multiple signalling
RT components.";
RL Plant J. 29:439-451(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-157.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF SER-389.
RX PubMed=20699401; DOI=10.1104/pp.110.157610;
RA Huang X., Li J., Bao F., Zhang X., Yang S.;
RT "A gain-of-function mutation in the Arabidopsis disease resistance gene
RT RPP4 confers sensitivity to low temperature.";
RL Plant Physiol. 154:796-809(2010).
CC -!- FUNCTION: TIR-NB-LRR receptor-like protein that confers resistance to
CC the pathogen Hyaloperonospora arabidopsis isolates Emoy2 and Emwa1
CC (downy mildew disease) (PubMed:11846877). Plays a role in the
CC regulation of temperature response during plant growth and survival
CC (PubMed:20699401). {ECO:0000269|PubMed:11846877,
CC ECO:0000269|PubMed:20699401}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- SUBUNIT: Interacts with RSH1. {ECO:0000269|PubMed:11846877}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM18462.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAC43672.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB10461.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80957.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF440696; AAM18462.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z97342; CAB10461.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161545; CAB80957.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83818.1; -; Genomic_DNA.
DR EMBL; AY120755; AAM53313.1; -; mRNA.
DR EMBL; AK119099; BAC43672.1; ALT_INIT; mRNA.
DR PIR; C71436; C71436.
DR RefSeq; NP_193420.2; NM_117790.4.
DR AlphaFoldDB; F4JNA9; -.
DR SMR; F4JNA9; -.
DR STRING; 3702.AT4G16860.1; -.
DR PaxDb; F4JNA9; -.
DR PRIDE; F4JNA9; -.
DR EnsemblPlants; AT4G16860.1; AT4G16860.1; AT4G16860.
DR GeneID; 827395; -.
DR Gramene; AT4G16860.1; AT4G16860.1; AT4G16860.
DR KEGG; ath:AT4G16860; -.
DR Araport; AT4G16860; -.
DR TAIR; locus:2129221; AT4G16860.
DR eggNOG; ENOG502QQJE; Eukaryota.
DR HOGENOM; CLU_001561_0_1_1; -.
DR InParanoid; F4JNA9; -.
DR OrthoDB; 1085197at2759; -.
DR PRO; PR:F4JNA9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JNA9; baseline and differential.
DR Genevisible; F4JNA9; AT.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0030275; F:LRR domain binding; ISS:TAIR.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0006952; P:defense response; TAS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; ISS:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11017; PTHR11017; 4.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF07725; LRR_3; 2.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Hydrolase; Leucine-rich repeat; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..1147
FT /note="Disease resistance protein RPP4"
FT /id="PRO_0000433379"
FT DOMAIN 11..175
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 189..446
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 548..573
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 584..606
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 608..629
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 630..653
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 655..676
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 698..721
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 722..743
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 744..766
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 767..790
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 792..813
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 814..836
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 837..860
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 926..950
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT REPEAT 952..973
FT /note="LRR 14"
FT /evidence="ECO:0000255"
FT REPEAT 974..996
FT /note="LRR 15"
FT /evidence="ECO:0000255"
FT REPEAT 997..1017
FT /note="LRR 16"
FT /evidence="ECO:0000255"
FT REPEAT 1018..1042
FT /note="LRR 17"
FT /evidence="ECO:0000255"
FT REPEAT 1044..1064
FT /note="LRR 18"
FT /evidence="ECO:0000255"
FT ACT_SITE 86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT MUTAGEN 389
FT /note="S->F: In the gain-of-function mutants chs2-1 and
FT chs2-2; seedling lethality when grown at temperatures below
FT 12 degrees Celsius. Dwarf plants with chlorotic leaves when
FT grown between 16 and 18 degrees Celsius. No visible
FT phenotype when grown at 22 degrees Celsius."
FT /evidence="ECO:0000269|PubMed:20699401"
FT CONFLICT 713
FT /note="G -> D (in Ref. 5; AAM53313)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1147 AA; 130429 MW; F86DBD8A5C9B7DA3 CRC64;
MASSSSSPSS RRYDVFPSFS GVDVRKTFLS HLIEALDRRS INTFMDHGIV RSCIIADALI
TAIREARISI VIFSENYASS TWCLNELVEI HKCYKKGEQM VIPVFYGVDP SHVRKQIGGF
GDVFKKTCED KPEDQKQRWV KALTDISNLA GEDLRNGPTE AFMVKKIAND VSNKLFPLPK
GFGDFVGIED HIKAIKSILC LESKEARIMV GIWGQSGIGK STIGRALFSQ LSSQFHHRAF
ITYKSTSGSD VSGMKLSWEK ELLSEILGQK DIKIDHFGVV EQRLKHKKVL ILLDDVDNLE
FLKTLVGKAE WFGSGSRIIV ITQDKQLLKA HEIDLVYEVE LPSQGLALKM ISQYAFGKDS
PPDDFKELAF EVAELVGSLP LGLSVLGSSL KGRDKDEWVK MMPRLRNDSD DKIEETLRVG
YDRLNKKNRE LFKCIACFFN GFKVSNVKEL LEDDVGLTML ADKSLIRITP DGDIEMHNLL
EKLGREIDRA KSKGNPAKRQ FLTNFEDIQE VVTEKTGTET VLGIRVPPTV LFSTRPLLVI
NEESFKGMRN LQYLEIGHWS EIGLWSEIGL WSKIDLPQGL VYLPLKLKLL KWNYCPLKSL
PSTFKAEYLV NLIMKYSKLE KLWEGTLPLG SLKKMDLGCS NNLKEIPDLS LAINLEELNL
SKCESLVTLP SSIQNAIKLR TLYCSGVLLI DLKSLEGMCN LEYLSVDWSS MEGTQGLIYL
PRKLKRLWWD YCPVKRLPSN FKAEYLVELR MENSDLEKLW DGTQPLGSLK EMYLHGSKYL
KEIPDLSLAI NLERLYLFGC ESLVTLPSSI QNATKLINLD MRDCKKLESF PTDLNLESLE
YLNLTGCPNL RNFPAIKMGC SYFEILQDRN EIEVEDCFWN KNLPAGLDYL DCLMRCMPCE
FRPEYLTFLD VSGCKHEKLW EGIQSLGSLK RMDLSESENL TEIPDLSKAT NLKRLYLNGC
KSLVTLPSTI GNLHRLVRLE MKECTGLELL PTDVNLSSLI ILDLSGCSSL RTFPLISTRI
ECLYLENTAI EEVPCCIEDL TRLSVLLMYC CQRLKNISPN IFRLTSLMVA DFTDCRGVIK
ALSDATVVAT MEDHVSCVPL SENIEYTCER FWDELYERNS RSIFSYKDED GDVYWVNWDL
MMMLMLI
