RPP8_ARATH
ID RPP8_ARATH Reviewed; 908 AA.
AC Q8W4J9; Q8GWG5; Q9M5A1; Q9ZSY3; Q9ZSY4;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Disease resistance protein RPP8;
DE AltName: Full=Resistance to Peronospora parasitica protein 8;
GN Name=RPP8; Synonyms=HRT; OrderedLocusNames=At5g43470; ORFNames=MWF20.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANTS RPP8-1; RPP8-2 AND
RP RPP8-3, AND VARIANTS.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=9811794; DOI=10.2307/3870909;
RA McDowell J.M., Dhandaydham M., Long T.A., Aarts M.G.M., Goff S.,
RA Holub E.B., Dangl J.L.;
RT "Intragenic recombination and diversifying selection contribute to the
RT evolution of downy mildew resistance at the RPP8 locus of Arabidopsis.";
RL Plant Cell 10:1861-1874(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS.
RC STRAIN=cv. Di-17;
RX PubMed=10810142; DOI=10.2307/3870993;
RA Cooley M.B., Pathirana S., Wu H.-J., Kachroo P., Klessig D.F.;
RT "Members of the Arabidopsis HRT/RPP8 family of resistance genes confer
RT resistance to both viral and oomycete pathogens.";
RL Plant Cell 12:663-676(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP INTERACTION WITH TIP.
RX PubMed=11041886; DOI=10.2307/3871202;
RA Ren T., Qu F., Morris T.J.;
RT "HRT gene function requires interaction between a NAC protein and viral
RT capsid protein to confer resistance to turnip crinkle virus.";
RL Plant Cell 12:1917-1926(2000).
RN [8]
RP INTERACTION WITH MORC1/CRT1.
RX PubMed=18191794; DOI=10.1016/j.chom.2007.11.006;
RA Kang H.-G., Kuhl J.C., Kachroo P., Klessig D.F.;
RT "CRT1, an Arabidopsis ATPase that interacts with diverse resistance
RT proteins and modulates disease resistance to turnip crinkle virus.";
RL Cell Host Microbe 3:48-57(2008).
RN [9]
RP INTERACTION WITH MORC1/CRT1.
RX PubMed=20332379; DOI=10.1105/tpc.109.071662;
RA Kang H.-G., Oh C.-S., Sato M., Katagiri F., Glazebrook J., Takahashi H.,
RA Kachroo P., Martin G.B., Klessig D.F.;
RT "Endosome-associated CRT1 functions early in resistance gene-mediated
RT defense signaling in Arabidopsis and tobacco.";
RL Plant Cell 22:918-936(2010).
RN [10]
RP INDUCTION BY HYALOPERONOSPORA ARABIDOPSIDIS; BTH; WOUNDING AND SALICYLIC
RP ACID, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20831409; DOI=10.1094/mpmi-01-10-0022;
RA Mohr T.J., Mammarella N.D., Hoff T., Woffenden B.J., Jelesko J.G.,
RA McDowell J.M.;
RT "The Arabidopsis downy mildew resistance gene RPP8 is induced by pathogens
RT and salicylic acid and is regulated by W box cis elements.";
RL Mol. Plant Microbe Interact. 23:1303-1315(2010).
RN [11]
RP SUBCELLULAR LOCATION, REGULATION BY BLUE-LIGHT AND DARKNESS, AND
RP INTERACTION WITH COP1.
RX PubMed=20624951; DOI=10.1073/pnas.1004529107;
RA Jeong R.-D., Chandra-Shekara A.C., Barman S.R., Navarre D., Klessig D.F.,
RA Kachroo A., Kachroo P.;
RT "Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated
RT viral defense by negatively regulating an E3 ubiquitin ligase.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:13538-13543(2010).
RN [12]
RP FUNCTION, AND INDUCTION BY SALICYLIC ACID.
RX PubMed=22072959; DOI=10.1371/journal.ppat.1002318;
RA Zhu S., Jeong R.-D., Venugopal S.C., Lapchyk L., Navarre D., Kachroo A.,
RA Kachroo P.;
RT "SAG101 forms a ternary complex with EDS1 and PAD4 and is required for
RT resistance signaling against turnip crinkle virus.";
RL PLoS Pathog. 7:E1002318-E1002318(2011).
CC -!- FUNCTION: Disease resistance protein. Resistance proteins guard the
CC plant against pathogens that contain an appropriate avirulence protein
CC via an indirect interaction with this avirulence protein. That triggers
CC a defense system including the hypersensitive response, which restricts
CC the pathogen growth. The interaction with TIP (TCV-interacting protein)
CC may be essential for the recognition of the avirulence proteins, and
CC the triggering of the defense response. Triggers resistance to turnip
CC crinkle virus (TCV) via a SAG101-dependent pathway.
CC {ECO:0000269|PubMed:22072959, ECO:0000269|PubMed:9811794}.
CC -!- SUBUNIT: Interacts with the NAC protein TIP (PubMed:11041886).
CC Interacts with MORC1/CRT1 (PubMed:18191794, PubMed:20332379). Interacts
CC with COP1 and is subsequently degraded in a 26s proteasome dependent
CC manner (PubMed:20624951). {ECO:0000269|PubMed:11041886,
CC ECO:0000269|PubMed:18191794, ECO:0000269|PubMed:20332379,
CC ECO:0000269|PubMed:20624951}.
CC -!- INTERACTION:
CC Q8W4J9; P43254: COP1; NbExp=2; IntAct=EBI-15865035, EBI-301649;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20624951}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8W4J9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8W4J9-2; Sequence=VSP_007171, VSP_007172;
CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves, and, to a lower extent,
CC in roots. {ECO:0000269|PubMed:20831409}.
CC -!- INDUCTION: By salicylic acid (SA) (PubMed:22072959, PubMed:20831409).
CC Induced by Hyaloperonospora arabidopsidis, benzothiadiazole (BTH) and
CC wounding (PubMed:20831409). Degraded in darkness and in blue-light
CC (PubMed:20624951). {ECO:0000269|PubMed:20624951,
CC ECO:0000269|PubMed:20831409, ECO:0000269|PubMed:22072959}.
CC -!- DOMAIN: The LRR repeats probably act as specificity determinant of
CC pathogen recognition. {ECO:0000269|PubMed:10810142}.
CC -!- POLYMORPHISM: The strong polymorphisms present in cv. Di-17 and cv.
CC Columbia are probably due to an unequal crossing-over between the
CC highly related RPP8 and RPH8A genes present in cv. Landsberg erecta.
CC Such variations probably modify the specificity of pathogen
CC recognition.
CC -!- MISCELLANEOUS: In cv. Columbia and cv. Landsberg erecta, RPP8
CC specifically recognizes the Emco5 avirulence protein from
CC Hyaloperonospora parasitica, while it is not the case in cv. Di-17,
CC where it confers resistance to Turnip Crinkle Virus upon recognition of
CC the viral capsid protein.
CC -!- MISCELLANEOUS: [Isoform 2]: Has been shown to exist only in cv.
CC Columbia so far. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family. RPP8/HRT
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AF089710; AAC83165.1; -; Genomic_DNA.
DR EMBL; AF089711; AAC78631.1; -; Genomic_DNA.
DR EMBL; AF234174; AAF36987.1; -; Genomic_DNA.
DR EMBL; AB025638; BAA97426.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94967.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94968.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71032.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71033.1; -; Genomic_DNA.
DR EMBL; AK118862; BAC43449.1; -; mRNA.
DR EMBL; AY062514; AAL32592.1; -; mRNA.
DR PIR; T48898; T48898.
DR PIR; T48899; T48899.
DR RefSeq; NP_001318736.1; NM_001344485.1. [Q8W4J9-1]
DR RefSeq; NP_001332592.1; NM_001344486.1. [Q8W4J9-1]
DR RefSeq; NP_199160.1; NM_123713.4. [Q8W4J9-1]
DR RefSeq; NP_851124.1; NM_180793.1. [Q8W4J9-1]
DR AlphaFoldDB; Q8W4J9; -.
DR SMR; Q8W4J9; -.
DR BioGRID; 19617; 8.
DR DIP; DIP-59355N; -.
DR IntAct; Q8W4J9; 2.
DR STRING; 3702.AT5G43470.2; -.
DR PaxDb; Q8W4J9; -.
DR PRIDE; Q8W4J9; -.
DR ProteomicsDB; 226905; -. [Q8W4J9-1]
DR EnsemblPlants; AT5G43470.1; AT5G43470.1; AT5G43470. [Q8W4J9-1]
DR EnsemblPlants; AT5G43470.2; AT5G43470.2; AT5G43470. [Q8W4J9-1]
DR EnsemblPlants; AT5G43470.3; AT5G43470.3; AT5G43470. [Q8W4J9-1]
DR EnsemblPlants; AT5G43470.4; AT5G43470.4; AT5G43470. [Q8W4J9-1]
DR GeneID; 834367; -.
DR Gramene; AT5G43470.1; AT5G43470.1; AT5G43470. [Q8W4J9-1]
DR Gramene; AT5G43470.2; AT5G43470.2; AT5G43470. [Q8W4J9-1]
DR Gramene; AT5G43470.3; AT5G43470.3; AT5G43470. [Q8W4J9-1]
DR Gramene; AT5G43470.4; AT5G43470.4; AT5G43470. [Q8W4J9-1]
DR KEGG; ath:AT5G43470; -.
DR Araport; AT5G43470; -.
DR TAIR; locus:2176486; AT5G43470.
DR eggNOG; KOG4658; Eukaryota.
DR HOGENOM; CLU_000837_25_4_1; -.
DR InParanoid; Q8W4J9; -.
DR OMA; LEYLRWF; -.
DR OrthoDB; 164862at2759; -.
DR PhylomeDB; Q8W4J9; -.
DR PRO; PR:Q8W4J9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8W4J9; baseline and differential.
DR Genevisible; Q8W4J9; AT.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; ISS:TAIR.
DR GO; GO:0071446; P:cellular response to salicylic acid stimulus; IEP:UniProtKB.
DR GO; GO:0006952; P:defense response; TAS:TAIR.
DR GO; GO:0051607; P:defense response to virus; IMP:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:TAIR.
DR GO; GO:0002230; P:positive regulation of defense response to virus by host; IDA:UniProtKB.
DR GO; GO:0009646; P:response to absence of light; IEP:UniProtKB.
DR GO; GO:0009637; P:response to blue light; IEP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IMP:TAIR.
DR GO; GO:0002239; P:response to oomycetes; IEP:UniProtKB.
DR GO; GO:0051707; P:response to other organism; IMP:TAIR.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR CDD; cd14798; RX-CC_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038005; RX-like_CC.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Cell membrane; Coiled coil;
KW Hypersensitive response; Leucine-rich repeat; Membrane; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..908
FT /note="Disease resistance protein RPP8"
FT /id="PRO_0000212719"
FT DOMAIN 146..459
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 575..600
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 601..623
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 648..673
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 693..718
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 722..746
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 748..770
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 793..820
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 842..867
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 882..905
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT COILED 15..57
FT /evidence="ECO:0000255"
FT BINDING 192..199
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 294..308
FT /note="WKMLLTSRNEGVGIH -> ELLWYIHEALFLLNS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_007171"
FT VAR_SEQ 309..908
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_007172"
FT VARIANT 27..31
FT /note="IDGQL -> VDEQI (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 29
FT /note="G -> E (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 85..87
FT /note="SGK -> RGE (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 88..91
FT /note="GKGV -> EKGI (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 100
FT /note="C -> R (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 121
FT /note="E -> D (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 129
FT /note="F -> L (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 133
FT /note="Q -> QQ (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 138
FT /note="G -> V (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 173
FT /note="K -> T (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 177
FT /note="G -> C (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 253
FT /note="Y -> S (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 257
FT /note="R -> P (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 265
FT /note="A -> T (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 267
FT /note="R -> K (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 270
FT /note="V -> L (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 283
FT /note="V -> R (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 369
FT /note="A -> V (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 387
FT /note="F -> S (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 399
FT /note="W -> C (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 426
FT /note="C -> R (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 429
FT /note="N -> F (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 436..446
FT /note="DSEISTYSLFY -> YSKISAYDLFN (in strain: cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 438..443
FT /note="EISTYS -> KITTQE (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 450
FT /note="A -> V (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 460
FT /note="E -> Q (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 480..486
FT /note="DNYLSWQ -> NKYLRVH (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 480..481
FT /note="DN -> NR (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 485..486
FT /note="WQ -> SH (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 489
FT /note="Y -> N (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 514
FT /note="I -> K (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 518
FT /note="C -> S (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 519
FT /note="T -> I (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 528
FT /note="S -> R (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 537
FT /note="S -> R (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 542
FT /note="H -> Q (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 543
FT /note="I -> L (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 547..550
FT /note="KNKT -> RNNA (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 549
FT /note="K -> N (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 558..565
FT /note="PRFEEDYW -> WDEDFG (in strain: cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 558..560
FT /note="PRF -> SRFK (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 564
FT /note="Y -> F (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 584
FT /note="S -> Y (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 595
FT /note="C -> S (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 609..612
FT /note="YEAK -> FLAG (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 630..635
FT /note="NLRVDT -> DLSVHE (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 632..636
FT /note="RVDTE -> SVNNK (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 650
FT /note="Q -> E (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 653..654
FT /note="YL -> HI (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 659
FT /note="Missing (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 660
FT /note="M -> K (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 676..678
FT /note="YLY -> FLF (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 678..679
FT /note="YG -> FR (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 692
FT /note="R -> H (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 697
FT /note="R -> Q (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 700
FT /note="A -> G (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 724
FT /note="T -> S (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 726..727
FT /note="NF -> YV (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 730..731
FT /note="SL -> TP (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 731..734
FT /note="LETY -> PEIF (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 752
FT /note="Q -> E (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 768
FT /note="F -> L (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 773..781
FT /note="VHLFLIYCG -> AQIYICNCR (in strain: cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 773..781
FT /note="VHLFLIYCG -> THIHLLFCR (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 800..807
FT /note="RLARKAFL -> QLTDEAFV (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 800..803
FT /note="RLAR -> KLTF (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 807..809
FT /note="LGS -> AGR (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 819
FT /note="P -> T (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 823..824
FT /note="VI -> AL (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 825
FT /note="E -> D (in strain: cv. Di-17)"
FT /evidence="ECO:0000269|PubMed:10810142"
FT VARIANT 828..829
FT /note="KE -> EQ (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:9811794"
FT VARIANT 851
FT /note="D -> H (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT VARIANT 854
FT /note="K -> E (in strain: cv. Di-17 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:10810142,
FT ECO:0000269|PubMed:9811794"
FT MUTAGEN 418
FT /note="D->N: In rpp8-1; loss of function."
FT /evidence="ECO:0000269|PubMed:9811794"
FT MUTAGEN 553
FT /note="R->K: In rpp8-2; loss of function."
FT /evidence="ECO:0000269|PubMed:9811794"
FT MUTAGEN 830
FT /note="S->L: In rpp8-1; loss of function."
FT /evidence="ECO:0000269|PubMed:9811794"
FT CONFLICT 83
FT /note="K -> R (in Ref. 6; AAL32592)"
FT /evidence="ECO:0000305"
FT CONFLICT 686
FT /note="S -> G (in Ref. 6; AAL32592)"
FT /evidence="ECO:0000305"
FT CONFLICT 835
FT /note="W -> R (in Ref. 6; AAL32592)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 908 AA; 104682 MW; 1BFA35BB6B0CB5CD CRC64;
MAEAFVSFGL EKLWDLLSRE SERLQGIDGQ LDGLKRQLRS LQSLLKDADA KKHGSDRVRN
FLEDVKDLVF DAEDIIESYV LNKLSGKGKG VKKHVRRLAC FLTDRHKVAS DIEGITKRIS
EVIGEMQSFG IQQIIDGGRS LSLQERQRVQ REIRQTYPDS SESDLVGVEQ SVKELVGHLV
ENDVHQVVSI AGMGGIGKTT LARQVFHHDL VRRHFDGFAW VCVSQQFTQK HVWQRILQEL
QPHDGDILQM DEYALQRKLF QLLEAGRYLV VLDDVWKKED WDVIKAVFPR KRGWKMLLTS
RNEGVGIHAD PTCLTFRASI LNPEESWKLC ERIVFPRRDE TEVRLDEEME AMGKEMVTHC
GGLPLAVKAL GGLLANKHTV PEWKRVFDNI GSQIVGGSWL DDNSLNSVYR ILSLSYEDLP
THLKHCFLNL AHFPEDSEIS TYSLFYYWAA EGIYDGSTIE DSGEYYLEEL VRRNLVIADD
NYLSWQSKYC QMHDMMREVC LSKAKEENFL QIIIDPTCTS TINAQSPSRS RRLSIHSGKA
FHILGHKNKT KVRSLIVPRF EEDYWIRSAS VFHNLTLLRV LDLSWVKFEG GKLPCSIGGL
IHLRYLSLYE AKVSHLPSTM RNLKLLLYLN LRVDTEEPIH VPNVLKEMIQ LRYLSLPLKM
DDKTKLELGD LVNLEYLYGF STQHSSVTDL LRMTKLRYLA VSLSERCNFE TLSSSLRELR
NLETLNFLFS LETYMVDYMG EFVLDHFIHL KQLGLAVRMS KIPDQHQFPP HLVHLFLIYC
GMEEDPMPIL EKLLHLKSVR LARKAFLGSR MVCSKGGFPQ LCVIEISKES ELEEWIVEEG
SMPCLRTLTI DDCKKLKELP DGLKYITSLK ELKIEGMKRE WKEKLVPGGE DYYKVQHIPD
VQFINCDQ
