ID   RPPA_STRGR              Reviewed;         372 AA.
AC   Q54240;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1999, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=1,3,6,8-tetrahydroxynaphthalene synthase {ECO:0000303|PubMed:16291687};
DE            Short=THNS {ECO:0000303|PubMed:16291687};
DE            EC=2.3.1.233 {ECO:0000269|PubMed:10476972};
DE   AltName: Full=1,3,6,8-tetrahydroxynaphthalene synthesis polyketide synthase type III {ECO:0000305};
GN   Name=rppA {ECO:0000303|PubMed:7649862};
GN   Synonyms=orf-1 {ECO:0000303|PubMed:7649862};
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7649862; DOI=10.7164/antibiotics.48.638;
RA   Ueda K., Kim K.M., Beppu T., Horinouchi S.;
RT   "Overexpression of a gene cluster encoding a chalcone synthase-like protein
RT   confers redbrown pigment production in Streptomyces griseus.";
RL   J. Antibiot. 48:638-646(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PATHWAY.
RC   STRAIN=IFO13350;
RX   PubMed=16291687; DOI=10.1128/jb.187.23.8149-8155.2005;
RA   Funa N., Funabashi M., Ohnishi Y., Horinouchi S.;
RT   "Biosynthesis of hexahydroxyperylenequinone melanin via oxidative aryl
RT   coupling by cytochrome P-450 in Streptomyces griseus.";
RL   J. Bacteriol. 187:8149-8155(2005).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF CYS-138, DISRUPTION PHENOTYPE,
RP   ACTIVE SITE, AND SUBUNIT.
RX   PubMed=10476972; DOI=10.1038/23748;
RA   Funa N., Ohnishi Y., Fujii I., Shibuya M., Ebizuka Y., Horinouchi S.;
RT   "A new pathway for polyketide synthesis in microorganisms.";
RL   Nature 400:897-899(1999).
CC   -!- FUNCTION: Involved in the biosynthesis of melanin but also various
CC       secondary metabolites containing a naphthoquinone ring. Catalyzes the
CC       iterative condensation of five CoA-linked malonyl units to form a
CC       pentaketide intermediate. THNS subsequently catalyzes the dual
CC       intramolecular Claisen and aldol condensations of this linear
CC       intermediate to produce the fused ring of 1,3,6,8-
CC       tetrahydroxynaphthalene (THN). {ECO:0000269|PubMed:10476972}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5 H(+) + 5 malonyl-CoA = 5 CO2 + 5 CoA + H2O + naphthalene-
CC         1,3,6,8-tetrol; Xref=Rhea:RHEA:41524, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:18365,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57384; EC=2.3.1.233;
CC         Evidence={ECO:0000269|PubMed:10476972};
CC   -!- PATHWAY: Pigment biosynthesis; melanin biosynthesis.
CC       {ECO:0000303|PubMed:16291687}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10476972}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are unable to produce
CC       melanin in hyphae, resulting in albino mycelium, however there is no
CC       effect on growth or morphological development.
CC       {ECO:0000269|PubMed:10476972}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AB018074; BAA33495.1; -; Genomic_DNA.
DR   EMBL; AB218878; BAE07216.1; -; Genomic_DNA.
DR   RefSeq; WP_012382077.1; NZ_UAVD01000021.1.
DR   AlphaFoldDB; Q54240; -.
DR   SMR; Q54240; -.
DR   PRIDE; Q54240; -.
DR   GeneID; 6209785; -.
DR   OMA; GTMEMLA; -.
DR   BioCyc; MetaCyc:MON-18700; -.
DR   UniPathway; UPA00785; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IDA:UniProtKB.
DR   GO; GO:0042438; P:melanin biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877; PTHR11877; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
PE   1: Evidence at protein level;
KW   Acyltransferase; Melanin biosynthesis; Transferase.
FT   CHAIN           1..372
FT                   /note="1,3,6,8-tetrahydroxynaphthalene synthase"
FT                   /id="PRO_0000430881"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000269|PubMed:10476972"
FT   MUTAGEN         138
FT                   /note="C->S: Abolishes the THN synthase activity."
SQ   SEQUENCE   372 AA;  40120 MW;  22CB05439472B9B5 CRC64;
     MATLCRPAIA VPEHVITMQQ TLDLARETHA GHPQRDLVLR LIQNTGVQTR HLVQPIEKTL
     AHPGFEVRNQ VYEAEAKTRV PEVVRRALAN AETEPSEIDL IVYVSCTGFM MPSLTAWIIN
     SMGFRPETRQ LPIAQLGCAA GGAAINRAHD FCVAYPDSNV LIVSCEFCSL CYQPTDIGVG
     SLLSNGLFGD ALSAAVVRGQ GGTGMRLERN GSHLVPDTED WISYAVRDTG FHFQLDKRVP
     GTMEMLAPVL LDLVDLHGWS VPNMDFFIVH AGGPRILDDL CHFLDLPPEM FRYSRATLTE
     RGNIASSVVF DALARLFDDG GAAESAQGLI AGFGPGITAE VAVGSWAKEG LGADVGRDLD
     ELELTAGVAL SG