AUKBB_XENLA
ID AUKBB_XENLA Reviewed; 368 AA.
AC Q6GPL3;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Aurora kinase B-B;
DE EC=2.7.11.1;
DE AltName: Full=Aurora/IPL1-related kinase 2-B;
DE Short=AIRK2-B;
DE Short=XAIRK2-B;
DE AltName: Full=Serine/threonine-protein kinase 12-B;
DE AltName: Full=Serine/threonine-protein kinase aurora-B-B;
DE Short=xAurora-B-B;
GN Name=aurkb-b; Synonyms=airk2-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Oocyte;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Serine/threonine-protein kinase component of the chromosomal
CC passenger complex (CPC), a complex that acts as a key regulator of
CC mitosis. The CPC complex has essential functions at the centromere in
CC ensuring correct chromosome alignment and segregation and is required
CC for chromatin-induced microtubule stabilization and spindle assembly.
CC Involved in the bipolar attachment of spindle microtubules to
CC kinetochores and is a key regulator for the onset of cytokinesis during
CC mitosis. Required for central/midzone spindle assembly and cleavage
CC furrow formation. Key component of the cytokinesis checkpoint, a
CC process required to delay abscission to prevent both premature
CC resolution of intercellular chromosome bridges and accumulation of DNA
CC damage. Phosphorylates 'Ser-10' of histone H3 during mitosis.
CC {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000250|UniProtKB:Q96GD4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000250|UniProtKB:Q96GD4};
CC -!- ACTIVITY REGULATION: Kinase activity is stimulated by cell-cycle
CC specific phosphorylation. {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SUBUNIT: Component of the chromosomal passenger complex (CPC).
CC {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96GD4}.
CC Chromosome {ECO:0000250|UniProtKB:Q96GD4}. Chromosome, centromere
CC {ECO:0000250|UniProtKB:Q96GD4}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q96GD4}. Midbody {ECO:0000250|UniProtKB:Q96GD4}.
CC Note=Localizes on chromosome arms and inner centromeres from prophase
CC through metaphase and then transferring to the spindle midzone and
CC midbody from anaphase through cytokinesis. Localization (and probably
CC targeting of the CPC) to the inner centromere occurs predominantly in
CC regions with overlapping mitosis-specific histone phosphorylations
CC H3pT3 and H2ApT12. {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. Aurora subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00159}.
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DR EMBL; BC073103; AAH73103.1; -; mRNA.
DR RefSeq; NP_001085657.1; NM_001092188.1.
DR AlphaFoldDB; Q6GPL3; -.
DR SMR; Q6GPL3; -.
DR BioGRID; 102246; 1.
DR BindingDB; Q6GPL3; -.
DR ChEMBL; CHEMBL3638361; -.
DR DNASU; 444083; -.
DR GeneID; 444083; -.
DR KEGG; xla:444083; -.
DR CTD; 444083; -.
DR Xenbase; XB-GENE-6256242; aurkb.S.
DR OrthoDB; 954262at2759; -.
DR Proteomes; UP000186698; Chromosome 3S.
DR Bgee; 444083; Expressed in egg cell and 15 other tissues.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005694; C:chromosome; ISS:UniProtKB.
DR GO; GO:0032133; C:chromosome passenger complex; ISS:UniProtKB.
DR GO; GO:0000775; C:chromosome, centromeric region; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0035175; F:histone kinase activity (H3-S10 specific); ISS:UniProtKB.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
DR GO; GO:0009838; P:abscission; ISS:UniProtKB.
DR GO; GO:0034644; P:cellular response to UV; ISS:UniProtKB.
DR GO; GO:0036089; P:cleavage furrow formation; ISS:UniProtKB.
DR GO; GO:0043988; P:histone H3-S28 phosphorylation; IEA:InterPro.
DR GO; GO:0044878; P:mitotic cytokinesis checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IEA:InterPro.
DR GO; GO:0051256; P:mitotic spindle midzone assembly; ISS:UniProtKB.
DR GO; GO:0002903; P:negative regulation of B cell apoptotic process; ISS:UniProtKB.
DR GO; GO:0032466; P:negative regulation of cytokinesis; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
DR GO; GO:0062033; P:positive regulation of mitotic sister chromatid segregation; ISS:UniProtKB.
DR GO; GO:0043687; P:post-translational protein modification; ISS:UniProtKB.
DR GO; GO:0034501; P:protein localization to kinetochore; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR030616; Aur.
DR InterPro; IPR028772; AURKB.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24350; PTHR24350; 1.
DR PANTHER; PTHR24350:SF4; PTHR24350:SF4; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Cytoplasm; Cytoskeleton; Kinase; Mitosis;
KW Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..368
FT /note="Aurora kinase B-B"
FT /id="PRO_0000281016"
FT DOMAIN 100..350
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 223
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 106..114
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 129
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 368 AA; 42015 MW; D1E79FB995701214 CRC64;
MSYKENLIPS SCSSSSSSSS KFATPSSATA AQRVLRKQPY VSIFTTPSDN LLAQRAQLAP
RVTPAASSSV PGRVALGTDV ASHNTALAEA PKRKFTIDDF DIGRPLGKGK FGNVYLAREK
QNKFIMALKV LFKSQLEKEG VEHQLRREIE IQSHLRHPNI LRMYNYFHDR KRIYLMLEFA
PRGELYKELQ KHGRFDEQRS ATFMEELADA LQYCHERKVI HRDIKPENLL MGYKGELKIA
DFGWSVHAPS LRRRTMCGTL DYLPPEMIEG KTHDEKVDLW CAGVLCYEFL VGMPPFDSPS
HSETHRRIVN VDLKFPPFLS EGSKDLISKL LRYHPAQRLP LKGVMEHPWV KANSRRVLPP
VYQSSHPK
