AUNIP_MOUSE
ID AUNIP_MOUSE Reviewed; 340 AA.
AC E9Q6Z5; B2RR86;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Aurora kinase A and ninein-interacting protein {ECO:0000305};
GN Name=Aunip {ECO:0000312|MGI:MGI:1917135};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: DNA-binding protein that accumulates at DNA double-strand
CC breaks (DSBs) following DNA damage and promotes DNA resection and
CC homologous recombination. Serves as a sensor of DNA damage: binds DNA
CC with a strong preference for DNA substrates that mimic structures
CC generated at stalled replication forks, and anchors RBBP8/CtIP to DSB
CC sites to promote DNA end resection and ensuing homologous recombination
CC repair. Inhibits non-homologous end joining (NHEJ). Required for the
CC dynamic movement of AURKA at the centrosomes and spindle apparatus
CC during the cell cycle. {ECO:0000250|UniProtKB:Q9H7T9}.
CC -!- SUBUNIT: Interacts (via C-terminus) with AURKA (via C-terminus).
CC Interacts (via N-terminus) with NIN; this interaction blocks NIN
CC phosphorylation by both AURKA and GSK3B. Identified in a complex with
CC NIN and AURKA. Interacts with RBBP8/CtIP.
CC {ECO:0000250|UniProtKB:Q9H7T9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H7T9}.
CC Chromosome {ECO:0000250|UniProtKB:Q9H7T9}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q9H7T9}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250|UniProtKB:Q9H7T9}. Note=Accumulates at sites of DNA damage
CC by binding to DNA substrates that mimick structures generated at
CC stalled replication forks. Localizes to the centrosome in interphase
CC and to the spindle pole in metaphase. {ECO:0000250|UniProtKB:Q9H7T9}.
CC -!- SIMILARITY: Belongs to the AUNIP family. {ECO:0000305}.
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DR EMBL; AL669982; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC138271; AAI38272.1; -; mRNA.
DR CCDS; CCDS51326.1; -.
DR RefSeq; NP_001074568.1; NM_001081099.1.
DR AlphaFoldDB; E9Q6Z5; -.
DR STRING; 10090.ENSMUSP00000101492; -.
DR iPTMnet; E9Q6Z5; -.
DR PhosphoSitePlus; E9Q6Z5; -.
DR EPD; E9Q6Z5; -.
DR PaxDb; E9Q6Z5; -.
DR PRIDE; E9Q6Z5; -.
DR ProteomicsDB; 273634; -.
DR Antibodypedia; 50871; 58 antibodies from 13 providers.
DR Ensembl; ENSMUST00000105866; ENSMUSP00000101492; ENSMUSG00000078521.
DR GeneID; 69885; -.
DR KEGG; mmu:69885; -.
DR UCSC; uc008vfg.1; mouse.
DR CTD; 79000; -.
DR MGI; MGI:1917135; Aunip.
DR VEuPathDB; HostDB:ENSMUSG00000078521; -.
DR eggNOG; ENOG502SFBZ; Eukaryota.
DR GeneTree; ENSGT00390000003280; -.
DR HOGENOM; CLU_835590_0_0_1; -.
DR InParanoid; E9Q6Z5; -.
DR OMA; HHRMGTP; -.
DR OrthoDB; 1235123at2759; -.
DR PhylomeDB; E9Q6Z5; -.
DR TreeFam; TF337334; -.
DR BioGRID-ORCS; 69885; 1 hit in 55 CRISPR screens.
DR ChiTaRS; Aunip; mouse.
DR PRO; PR:E9Q6Z5; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; E9Q6Z5; protein.
DR Bgee; ENSMUSG00000078521; Expressed in animal zygote and 102 other tissues.
DR Genevisible; E9Q6Z5; MM.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0090734; C:site of DNA damage; ISS:UniProtKB.
DR GO; GO:0000922; C:spindle pole; ISS:UniProtKB.
DR GO; GO:0003684; F:damaged DNA binding; ISS:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:2001033; P:negative regulation of double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
DR GO; GO:0007051; P:spindle organization; ISS:UniProtKB.
DR InterPro; IPR029286; AUNIP.
DR PANTHER; PTHR14526; PTHR14526; 1.
DR Pfam; PF15334; AIB; 1.
PE 2: Evidence at transcript level;
KW Chromosome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..340
FT /note="Aurora kinase A and ninein-interacting protein"
FT /id="PRO_0000419630"
FT REGION 175..340
FT /note="Interaction with AURKA"
FT /evidence="ECO:0000250|UniProtKB:Q9H7T9"
FT REGION 178..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..340
FT /note="Interaction with RBBP8/CtIP"
FT /evidence="ECO:0000250|UniProtKB:Q9H7T9"
FT REGION 293..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 277
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7T9"
FT CONFLICT 3
FT /note="H -> R (in Ref. 2; AAI38272)"
FT /evidence="ECO:0000305"
FT CONFLICT 147
FT /note="D -> G (in Ref. 2; AAI38272)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="Missing (in Ref. 2; AAI38272)"
FT /evidence="ECO:0000305"
FT CONFLICT 166..168
FT /note="YLE -> DLG (in Ref. 2; AAI38272)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="Q -> R (in Ref. 2; AAI38272)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 37684 MW; 0072CC037A118F8D CRC64;
MRHRGPEEEA CGVWLDAAAL KRRKMQTHLI KLGTKMLPLL PGERKPNDPF TQRRGTRQTS
IASFVTLQSG MASGGNQKNI FSLKENQTNK ECKRTQLDCL DDGLLSPLVT SAPADIQEAG
HPRSPQISGC QGLEMSSLTM MSLTQPDVLM GTGESKGPLD SSFSQYLERS CLLDQREAKR
KGEGLRESKT DCPGMGSHIR PPGSKCHQPL DKAEMGKRGP TKENRQAPVH LQTYRSGSCS
RKKTLLVTES PCPLSLFPWD SERSDRDSWS QLFTEDSQGQ QVIAHNTKMP FRDVTNARNQ
GSGQFPDSPQ AQGQDGPAQL HLQSYLLFTQ DSEGNRVIRH
