ID   AUP1_DANRE              Reviewed;         423 AA.
AC   Q6PBN5; Q6P0U2;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Lipid droplet-regulating VLDL assembly factor AUP1 {ECO:0000250|UniProtKB:Q9Y679};
DE   AltName: Full=Ancient ubiquitous protein 1 {ECO:0000250|UniProtKB:Q9Y679};
GN   Name=aup1 {ECO:0000250|UniProtKB:Q9Y679};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in the translocation of terminally misfolded
CC       proteins from the endoplasmic reticulum lumen to the cytoplasm and
CC       their degradation by the proteasome (By similarity). Plays a role in
CC       lipid droplet formation (By similarity). Induces lipid droplet
CC       clustering (By similarity). {ECO:0000250|UniProtKB:Q9Y679}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q9Y679}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y679}. Lipid droplet
CC       {ECO:0000250|UniProtKB:Q9Y679}.
CC   -!- SIMILARITY: Belongs to the AUP1 family. {ECO:0000305}.
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DR   EMBL; BC065447; AAH65447.1; -; mRNA.
DR   EMBL; BC059643; AAH59643.1; -; mRNA.
DR   RefSeq; NP_955984.2; NM_199690.2.
DR   AlphaFoldDB; Q6PBN5; -.
DR   SMR; Q6PBN5; -.
DR   STRING; 7955.ENSDARP00000054866; -.
DR   PaxDb; Q6PBN5; -.
DR   Ensembl; ENSDART00000054867; ENSDARP00000054866; ENSDARG00000092609.
DR   GeneID; 324654; -.
DR   KEGG; dre:324654; -.
DR   CTD; 550; -.
DR   ZFIN; ZDB-GENE-030131-3375; aup1.
DR   eggNOG; KOG2898; Eukaryota.
DR   GeneTree; ENSGT00390000016110; -.
DR   HOGENOM; CLU_045696_0_0_1; -.
DR   InParanoid; Q6PBN5; -.
DR   OMA; CLMLIRI; -.
DR   OrthoDB; 1319689at2759; -.
DR   PhylomeDB; Q6PBN5; -.
DR   TreeFam; TF313372; -.
DR   PRO; PR:Q6PBN5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 14.
DR   Bgee; ENSDARG00000092609; Expressed in muscle tissue and 28 other tissues.
DR   ExpressionAtlas; Q6PBN5; baseline and differential.
DR   GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IBA:GO_Central.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0097027; F:ubiquitin-protein transferase activator activity; IBA:GO_Central.
DR   GO; GO:0071712; P:ER-associated misfolded protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0140042; P:lipid droplet formation; ISS:UniProtKB.
DR   GO; GO:0034389; P:lipid droplet organization; ISS:UniProtKB.
DR   GO; GO:1990044; P:protein localization to lipid droplet; ISS:UniProtKB.
DR   GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISS:UniProtKB.
DR   InterPro; IPR003892; CUE.
DR   Pfam; PF02845; CUE; 1.
DR   SMART; SM00546; CUE; 1.
DR   PROSITE; PS51140; CUE; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Lipid droplet; Membrane; Reference proteome.
FT   CHAIN           1..423
FT                   /note="Lipid droplet-regulating VLDL assembly factor AUP1"
FT                   /id="PRO_0000359871"
FT   TOPO_DOM        1..19
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   INTRAMEM        20..40
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   TOPO_DOM        41..423
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   DOMAIN          304..346
FT                   /note="CUE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
FT   REGION          355..392
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        361..377
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        182
FT                   /note="D -> E (in Ref. 1; AAH59643)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   423 AA;  47414 MW;  53FFFCF5553FA067 CRC64;
     METRGIEQMF DFQRLPNDRF ILLLLLLYAP VGFCLMLLRI FIGVHVFLVS CALPDSIVRR
     FIVRIMCSVL GLHVQQNSPR LRDKTTRLYV CNHVTHFDHN IINLLTSCNT PLLEGPVGFL
     CWARGFMELG QGVGSRTELT ETLHRYCSSP DTLPLLLFPE EDTTNGRTGL LKFSSWPFSV
     SDSIQPVALL VKRPFIAVST PESSWLTELL WTFFVPFTVY HVRWLPPLSK EDGETHQEFA
     SKVQGLLATE LGVISTQITK ADKAEHIKRK RHSAPQTAHS NLGARPRTVA QGFLGTSVGA
     EDSRIARLAQ QVKEVLPDVP VSVITRDLLQ TNCVDTTITN LLERTDQFNS EAAMTMPSGP
     GKAAASSTPS AMVSSPNLKP AAKSFGRSPI DRHMSLQERK EALYEYARRR YIEKHGLNKE
     DDL