ID   AUP1_XENTR              Reviewed;         403 AA.
AC   A9ULG4;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Lipid droplet-regulating VLDL assembly factor AUP1 {ECO:0000250|UniProtKB:Q9Y679};
DE   AltName: Full=Ancient ubiquitous protein 1 {ECO:0000250|UniProtKB:Q9Y679};
GN   Name=aup1 {ECO:0000250|UniProtKB:Q9Y679};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Lung;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in the translocation of terminally misfolded
CC       proteins from the endoplasmic reticulum lumen to the cytoplasm and
CC       their degradation by the proteasome (By similarity). Plays a role in
CC       lipid droplet formation (By similarity). Induces lipid droplet
CC       clustering (By similarity). {ECO:0000250|UniProtKB:Q9Y679}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q9Y679}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9Y679}. Lipid droplet
CC       {ECO:0000250|UniProtKB:Q9Y679}.
CC   -!- SIMILARITY: Belongs to the AUP1 family. {ECO:0000305}.
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DR   EMBL; BC157245; AAI57246.1; -; mRNA.
DR   RefSeq; NP_001107375.1; NM_001113903.1.
DR   AlphaFoldDB; A9ULG4; -.
DR   SMR; A9ULG4; -.
DR   STRING; 8364.ENSXETP00000058668; -.
DR   GeneID; 100135201; -.
DR   KEGG; xtr:100135201; -.
DR   CTD; 550; -.
DR   Xenbase; XB-GENE-6457109; aup1.
DR   eggNOG; KOG2898; Eukaryota.
DR   InParanoid; A9ULG4; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0000839; C:Hrd1p ubiquitin ligase ERAD-L complex; IBA:GO_Central.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0097027; F:ubiquitin-protein transferase activator activity; IBA:GO_Central.
DR   GO; GO:0071712; P:ER-associated misfolded protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0140042; P:lipid droplet formation; ISS:UniProtKB.
DR   GO; GO:0034389; P:lipid droplet organization; ISS:UniProtKB.
DR   GO; GO:1990044; P:protein localization to lipid droplet; ISS:UniProtKB.
DR   GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISS:UniProtKB.
DR   InterPro; IPR003892; CUE.
DR   Pfam; PF02845; CUE; 1.
DR   SMART; SM00546; CUE; 1.
DR   PROSITE; PS51140; CUE; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Lipid droplet; Membrane; Reference proteome.
FT   CHAIN           1..403
FT                   /note="Lipid droplet-regulating VLDL assembly factor AUP1"
FT                   /id="PRO_0000359873"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   INTRAMEM        22..42
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   TOPO_DOM        43..399
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y679"
FT   DOMAIN          286..328
FT                   /note="CUE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
SQ   SEQUENCE   403 AA;  45389 MW;  679DE14DD6924486 CRC64;
     MEQPSVESLL ELRRFPRKQL SLILLLLYSP LGLCLFLIRL FIGAHVFLVS CVLPDSVFRR
     FLLRVMSSVL GVYVSHSALR PLERRGKILI CNHRTDFDHN IISLIAPCCS PSLSCAPGFL
     CWARGFLELG ALGSRTQLME SLKHYLSQPG GGPLLLFPEE ETTSGRTGLL HFSSWPFSLS
     DSVQPLTLTV QRPLVAAAVS GCSWVTELFW LLFIPFTVYQ VRWLPPVTRH TRESDEEFAF
     RVQQMMAGSL GVAATRHTGA DRAEYLKRRR TELPRSAPRS VPLSPTQMQM AQHVKEVLPQ
     VPLSAIHRDL GHTGCIDTTI TNFLEGRVTF LPEEETLGGN EATERTPLDR ISRPLPRGFA
     KSPEVRHLSL QERKEALYEC ARRKYLEKFG SVGREEEEKG ARG