ID   AUPB_MARN1              Reviewed;        1063 AA.
AC   H8WEC0;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Alkane uptake protein B {ECO:0000303|PubMed:29871914};
DE   Flags: Precursor;
GN   Name=aupB {ECO:0000303|PubMed:29871914};
GN   ORFNames=MARHY0477 {ECO:0000312|EMBL:CCG93976.1};
OS   Marinobacter nauticus (strain ATCC 49840 / DSM 8798 / CIP 103578 / SP17)
OS   (Marinobacter hydrocarbonoclasticus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Marinobacteraceae; Marinobacter.
OX   NCBI_TaxID=1163748;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49840 / DSM 8798 / CIP 103578 / SP17;
RX   PubMed=22689231; DOI=10.1128/jb.00500-12;
RA   Grimaud R., Ghiglione J.F., Cagnon C., Lauga B., Vaysse P.J.,
RA   Rodriguez-Blanco A., Mangenot S., Cruveiller S., Barbe V., Duran R.,
RA   Wu L.F., Talla E., Bonin P., Michotey V.;
RT   "Genome sequence of the marine bacterium Marinobacter hydrocarbonoclasticus
RT   SP17, which forms biofilms on hydrophobic organic compounds.";
RL   J. Bacteriol. 194:3539-3540(2012).
RN   [2]
RP   FUNCTION, INTERACTION WITH AUPA, SUBCELLULAR LOCATION, INDUCTION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 49840 / DSM 8798 / CIP 103578 / SP17;
RX   PubMed=29871914; DOI=10.1128/mbio.00520-18;
RA   Mounier J., Hakil F., Branchu P., Naitali M., Goulas P., Sivadon P.,
RA   Grimaud R.;
RT   "AupA and AupB are outer and inner membrane proteins involved in alkane
RT   uptake in Marinobacter hydrocarbonoclasticus SP17.";
RL   MBio 9:E00520-E00520(2018).
CC   -!- FUNCTION: Required for growth on alkanes. Probably involved in the
CC       uptake of micelle-solubilized alkanes. May facilitate the transfer of
CC       alkanes from the outer membrane to the inner membrane.
CC       {ECO:0000269|PubMed:29871914}.
CC   -!- SUBUNIT: Interacts with the outer membrane protein AupA.
CC       {ECO:0000269|PubMed:29871914}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:29871914}; Lipid-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}.
CC   -!- INDUCTION: Expression increases during biofilm formation on n-
CC       hexadecane. Forms an operon with aupA. {ECO:0000269|PubMed:29871914}.
CC   -!- DISRUPTION PHENOTYPE: Mutants show a lower rate of biofilm formation on
CC       solid paraffin and on the liquid alkane n-hexadecane, while growth on
CC       nonalkane substrates was not affected. Planktonic growth on water-
CC       soluble substrates is not affected. Mutants are impaired in the
CC       assimilation of n-hexadecane solubilized in surfactant micelles.
CC       {ECO:0000269|PubMed:29871914}.
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DR   EMBL; FO203363; CCG93976.1; -; Genomic_DNA.
DR   RefSeq; WP_014420411.1; NC_017067.1.
DR   AlphaFoldDB; H8WEC0; -.
DR   EnsemblBacteria; CCG93976; CCG93976; MARHY0477.
DR   GeneID; 31820000; -.
DR   KEGG; mhc:MARHY0477; -.
DR   PATRIC; fig|2743.3.peg.468; -.
DR   HOGENOM; CLU_278074_0_0_6; -.
DR   Proteomes; UP000007884; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.1220; -; 1.
DR   InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR   InterPro; IPR032812; SbsA_Ig.
DR   Pfam; PF13205; Big_5; 2.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW   Signal; Transport.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..1063
FT                   /note="Alkane uptake protein B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT                   /id="PRO_0000445086"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   1063 AA;  113828 MW;  E876A637D5CECE6B CRC64;
     MKYNKTLALI PAILLAACGG GDKQSIDEKP RPGSMVYSFP MDGQADVSPK ADIVLRFSHA
     ITDDEATLQN KIILQSSGQS QPFTVTLIDS GKSLKLEPAN PLATGEEFTV TFQEPLAAEG
     GRQITTPNAT GDDGIQFATR GAYAGLAELA NTADTFDIAW QVPASDSPFQ AMNFSTFRFA
     MTQPVHPEWQ SLGGSIQLRD ASGQEVPATV LVKGNRITVD PCVTPEPSQC GSKQDILNTG
     ETYTLQLTNL ASLTDSSEEN RFTGEFTFTP RDTGPTVVLQ QTAVDSGNGE LTSVLNGQAL
     NAVTLNSVLQ GEAGPSQQTG DLFAELAYAP AFDADEALPL RIPKGSVLKS TSLNVLVGGK
     VQVLDAATGE DQQTGTIKVT MLSDASGYMS PNQYTDDINA PRHITLFMDV SMNTEAAQPN
     AALSQDLMGV ELRGIALVRD GVLTIDAIGM VEPNLLGQEF TDSTIAFHLQ AATDADSVLD
     AENLRELDAT PPSLVSWMPG PANAVPATRQ SMQRPGDPII LFFDEPLDPA SVENGVELIE
     NGAPVTNLQA RLDGTALVLN PQGGLKHGVP YSVSVDGLTD LAGNRAMVSP LSFELDSIEE
     NGSPGNKGFP LALTTYPGYP CETDYENTLD LENGVFGKCW TADANNAGDV LPVSKMPADR
     PITVVFSKSL DLDSVIVGET FTVQEIAQEA NGTVTVLNQQ VAGRLEKNNQ RIRFYPEQPW
     QVGAHYRYIL ASSEQSGTCT PGQYTSICDE DGIPLKTDLL EGLNDGDGNN GPDNLEIVFT
     GSEARSTVFT PLRNLPIRDT NSNLAIDCDD LGSESCLEPF NHQGSDSEGF LPSANAAKLL
     VTQDHAEDAR VGCAVDGADC PRKKFIYQTY ALNTEVIGPT VDPDTGRDAV KVLLYPTQLA
     TTSLDVHLSL LSTVSSTGPQ VLRMRYAKDD PECTGASCAR NSLIPGYITE NDQGETIFKT
     KAELFLDAPG LKATASILGI PTDLPLTHNL FGYPFTLELE GRVVFFDDGR MQIEQRNFNV
     PHIDVEAVAL GIINTEIPLI IPEQGVYLNF ISNPVKEIPA QYE