RPRM_MOUSE
ID RPRM_MOUSE Reviewed; 109 AA.
AC Q9JJ72; Q8K1G8;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein reprimo;
GN Name=Rprm;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND
RP GLYCOSYLATION AT ASN-7 AND ASN-18.
RX PubMed=10930422; DOI=10.1074/jbc.c000235200;
RA Ohki R., Nemoto J., Murasawa H., Oda E., Inazawa J., Tanaka N.,
RA Taniguchi T.;
RT "Reprimo, a new candidate mediator of the p53-mediated cell cycle arrest at
RT the G2 phase.";
RL J. Biol. Chem. 275:22627-22630(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic stem cell, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in the regulation of p53-dependent G2 arrest
CC of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDK1
CC activity and nuclear translocation of the CDC2 cyclin B1 complex.
CC {ECO:0000269|PubMed:10930422}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10930422}. Membrane
CC {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC -!- INDUCTION: By p53/TP53, following X-ray irradiation.
CC {ECO:0000269|PubMed:10930422}.
CC -!- MISCELLANEOUS: 'Reprimo' signifies stop/repress.
CC -!- SIMILARITY: Belongs to the reprimo family. {ECO:0000305}.
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DR EMBL; AB043586; BAB01514.1; -; mRNA.
DR EMBL; AK010465; BAB26960.1; -; mRNA.
DR EMBL; AK014769; BAB29541.1; -; mRNA.
DR EMBL; AL844850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030065; AAH30065.1; -; mRNA.
DR CCDS; CCDS16040.1; -.
DR RefSeq; NP_075885.1; NM_023396.5.
DR AlphaFoldDB; Q9JJ72; -.
DR STRING; 10090.ENSMUSP00000097667; -.
DR GlyGen; Q9JJ72; 2 sites.
DR iPTMnet; Q9JJ72; -.
DR PhosphoSitePlus; Q9JJ72; -.
DR PaxDb; Q9JJ72; -.
DR PRIDE; Q9JJ72; -.
DR ProteomicsDB; 299950; -.
DR Antibodypedia; 54148; 90 antibodies from 15 providers.
DR DNASU; 67874; -.
DR Ensembl; ENSMUST00000100089; ENSMUSP00000097667; ENSMUSG00000075334.
DR GeneID; 67874; -.
DR KEGG; mmu:67874; -.
DR UCSC; uc008jrp.1; mouse.
DR CTD; 56475; -.
DR MGI; MGI:1915124; Rprm.
DR VEuPathDB; HostDB:ENSMUSG00000075334; -.
DR eggNOG; ENOG502S262; Eukaryota.
DR GeneTree; ENSGT00390000010523; -.
DR HOGENOM; CLU_170456_0_0_1; -.
DR InParanoid; Q9JJ72; -.
DR OMA; ERNLFIM; -.
DR OrthoDB; 1529294at2759; -.
DR PhylomeDB; Q9JJ72; -.
DR TreeFam; TF332720; -.
DR BioGRID-ORCS; 67874; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Rprm; mouse.
DR PRO; PR:Q9JJ72; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9JJ72; protein.
DR Bgee; ENSMUSG00000075334; Expressed in piriform cortex and 171 other tissues.
DR Genevisible; Q9JJ72; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051726; P:regulation of cell cycle; IDA:MGI.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; IDA:MGI.
DR InterPro; IPR033356; Reprimo.
DR InterPro; IPR043383; Reprimo_fam.
DR PANTHER; PTHR28649; PTHR28649; 1.
DR PANTHER; PTHR28649:SF2; PTHR28649:SF2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..109
FT /note="Protein reprimo"
FT /id="PRO_0000312753"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 98
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:10930422"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:10930422"
FT CONFLICT 17
FT /note="V -> A (in Ref. 4; AAH30065)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 109 AA; 11820 MW; 954F7C6397A84F9E CRC64;
MNSVLGNQTD VAGLFLVNSS EALERAVRCC TQASVVTDDG FAEGGPDERS LYIMRVVQIA
VMCVLSLTVV FGIFFLGCNL LIKSEGMINF LVKDRRPSKE VEAVVVGPY
