RPS4L_ARATH
ID RPS4L_ARATH Reviewed; 1217 AA.
AC Q9SCX6;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Disease resistance protein RPS4 {ECO:0000303|PubMed:10571887};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Resistance to Pseudomonas syringae 4 {ECO:0000303|PubMed:10571887};
GN Name=RPS4 {ECO:0000303|PubMed:10571887};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:CAB53785.1};
RN [1] {ECO:0000312|EMBL:CAB53785.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10571887; DOI=10.1046/j.1365-313x.1999.t01-1-00600.x;
RA Gassmann W., Hinsch M.E., Staskawicz B.J.;
RT "The Arabidopsis RPS4 bacterial-resistance gene is a member of the TIR-NBS-
RT LRR family of disease-resistance genes.";
RL Plant J. 20:265-277(1999).
RN [2]
RP FUNCTION, ALTERNATIVE SPLICING, AND MUTAGENESIS OF ASN-195 AND TYR-950.
RX PubMed=14523247; DOI=10.1105/tpc013474;
RA Zhang X.C., Gassmann W.;
RT "RPS4-mediated disease resistance requires the combined presence of RPS4
RT transcripts with full-length and truncated open reading frames.";
RL Plant Cell 15:2333-2342(2003).
RN [3]
RP ALTERNATIVE SPLICING, AND INDUCTION BY AVRRPS4.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
RX PubMed=17951452; DOI=10.1104/pp.107.108720;
RA Zhang X.C., Gassmann W.;
RT "Alternative splicing and mRNA levels of the disease resistance gene RPS4
RT are induced during defense responses.";
RL Plant Physiol. 145:1577-1587(2007).
CC -!- FUNCTION: Disease resistance (R) protein that specifically recognizes
CC the AvrRps4 type III effector avirulence protein from Pseudomonas
CC syringae. Resistance proteins guard the plant against pathogens that
CC contain an appropriate avirulence protein via an indirect interaction
CC with this avirulence protein. That triggers a defense system including
CC the hypersensitive response, which restricts the pathogen growth. The
CC combined presence of both regular and alternative RPS4 transcripts with
CC truncated open reading frames (ORFs) is necessary for function. RPS4
CC function is regulated at multiple levels, including gene expression,
CC alternative splicing, and protein stability.
CC {ECO:0000269|PubMed:14523247, ECO:0000269|PubMed:17951452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- SUBUNIT: Interacts with EDS1. {ECO:0000250|UniProtKB:Q9XGM3}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. Alternative transcripts
CC are necessary but not sufficient for resistance.;
CC Name=1;
CC IsoId=Q9SCX6-1; Sequence=Displayed;
CC -!- INDUCTION: Up-regulated by AvrRps4 in an EDS1-dependent manner.
CC {ECO:0000269|PubMed:17951452}.
CC -!- DOMAIN: The TIR domain is a signaling domain involved in cell death
CC induction. {ECO:0000250|UniProtKB:Q9XGM3}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- MISCELLANEOUS: Only two amino-acid changes (N195D and Y950H) are linked
CC to a change from a resistant strain (cv. Columbia or cv. Landsberg
CC erecta) to a susceptible one (cv. RLD). {ECO:0000269|PubMed:10571887}.
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DR EMBL; AJ249264; CAB53785.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9SCX6; -.
DR SMR; Q9SCX6; -.
DR ExpressionAtlas; Q9SCX6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR045344; C-JID.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF20160; C-JID; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Leucine-rich repeat; NAD; Nucleus;
KW Plant defense; Repeat.
FT CHAIN 1..1217
FT /note="Disease resistance protein RPS4"
FT /id="PRO_0000431386"
FT DOMAIN 14..175
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 211..472
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 260..285
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 436..459
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 614..636
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 682..706
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 708..728
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 729..749
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 750..774
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 796..818
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 819..842
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 861..887
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REGION 1162..1195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1170..1177
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT ACT_SITE 88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT MUTAGEN 195
FT /note="N->D: Loss of resistance. Loss of resistance; when
FT associated with H-950."
FT /evidence="ECO:0000269|PubMed:14523247"
FT MUTAGEN 950
FT /note="Y->H: Loss of resistance. Loss of resistance; when
FT associated with D-195."
FT /evidence="ECO:0000269|PubMed:14523247"
SQ SEQUENCE 1217 AA; 137761 MW; 34FD6949A2E2AEB5 CRC64;
METSSISTVE DKPPQHQVFI NFRGADLRRR FVSHLVTALK LNNINVFIDD YEDRGQPLDV
LLKRIEESKI VLAIFSGNYT ESVWCVRELE KIKDCTDEGT LVAIPIFYKL EPSTVRDLKG
KFGDRFRSMA KGDERKKKWK EAFNLIPNIM GIIIDKKSVE SEKVNEIVKA VKTALTGIPP
EGSHNAVVGA LGNSNAGTSS GDKKHETFGN EQRLKDLEEK LDRDKYKGTR IIGVVGMPGI
GKTTLLKELY KTWQGKFSRH ALIDQIRVKS KHLELDRLPQ MLLGELSKLN NPHVDNLKDP
YSQLHERKVL VVLDDVSKRE QIDALREILD WIKEGKEGSR VVIATSDMSL TNGLVDDTYM
VQNLNHRDSL QLFHYHAFID DQANPQKKDF MKLSEGFVHY ARGHPLALKV LGGELNKKSM
DHWNSKMKKL AQSPSPNIVS VFQVSYDELT TAQKDAFLDI ACFRSQDKDY VESLLASSDL
GSAEAMSAVK SLTDKFLINT CDGRVEMHDL LYKFSREVDL KASNQDGSRQ RRLWLHQHII
KGGIINVLQN KMKAANVRGI FLDLSEVEDE TSLDRDHFIN MGNLRYLKFY NSHCPQECKT
NNKINIPDKL KLPLKEVRCL HWLKFPLETL PNDFNPINLV DLKLPYSETE QLWEGDKDTP
CLRWVDLNHS SKLCSLSGLS KAEKLQRLNL EGCTTLKAFP HDMKKMKMLA FLNLKGCTSL
ESLPEMNLIS LKTLTLSGCS TFKEFPLISD NIETLYLDGT AISQLPMNME KLQRLVVLNM
KDCKMLEEIP GRVGELKALQ ELILSDCLNL KIFPEIDISF LNILLLDGTA IEVMPQLPSV
QYLCLSRNAK ISCLPVGISQ LSQLKWLDLK YCTSLTSVPE FPPNLQCLDA HGCSSLKTVS
KPLARIMPTE QNHSTFIFTN CENLEQAAKE EITSYAQRKC QLLSYARKRY NGGLVSESLF
STCFPGCEVP SWFCHETVGS ELEVKLLPHW HDKKLAGIAL CAVISCLDPQ DQVSRLSVTC
TFKVKDEDKS WVPYTCPVGS WTRHGGGKDK IELDHVFIGY TSCPHTIKCH EEGNSDECNP
TEASLKFTVT GGTSENGKYK VFKCGLSLVY AKDKDKNSAL ETKYDMLIGK SFQETSEGVD
GRVKKTKGKY VMPVEKNFQE TTEGVDGRVK KKKKTRMDNG RPKKKQRSGR DDNQTRMQVE
LQEGNINSVI MHTVKNF
