RPS4R_ARATH
ID RPS4R_ARATH Reviewed; 1217 AA.
AC Q9SCX7;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Inactive disease resistance protein RPS4 {ECO:0000303|PubMed:10571887};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Resistance to Pseudomonas syringae 4 {ECO:0000303|PubMed:10571887};
GN Name=RPS4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:CAB53784.1};
RN [1] {ECO:0000312|EMBL:CAB53784.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. RLD;
RX PubMed=10571887; DOI=10.1046/j.1365-313x.1999.t01-1-00600.x;
RA Gassmann W., Hinsch M.E., Staskawicz B.J.;
RT "The Arabidopsis RPS4 bacterial-resistance gene is a member of the TIR-NBS-
RT LRR family of disease-resistance genes.";
RL Plant J. 20:265-277(1999).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- SUBUNIT: Interacts with EDS1. {ECO:0000250|UniProtKB:Q9XGM3}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- MISCELLANEOUS: Only two amino-acid changes (D195N and H950Y) are linked
CC to a change from a susceptible strain (cv. RLD) to a resistant one (cv.
CC Columbia or cv. Landsberg erecta). {ECO:0000269|PubMed:10571887}.
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DR EMBL; AJ249263; CAB53784.1; -; Genomic_DNA.
DR PIR; T51141; T51141.
DR AlphaFoldDB; Q9SCX7; -.
DR SMR; Q9SCX7; -.
DR ExpressionAtlas; Q9SCX7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR045344; C-JID.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF20160; C-JID; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 3: Inferred from homology;
KW Hydrolase; Leucine-rich repeat; NAD; Nucleus; Repeat.
FT CHAIN 1..1217
FT /note="Inactive disease resistance protein RPS4"
FT /id="PRO_0000431385"
FT DOMAIN 14..175
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 211..472
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 260..285
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 436..459
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 614..636
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 637..659
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 682..706
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 708..728
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 729..749
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 750..774
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 796..818
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 819..842
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 861..887
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REGION 1162..1195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1170..1177
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT ACT_SITE 88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
SQ SEQUENCE 1217 AA; 137718 MW; 1540746363A3680A CRC64;
METSSISTVE DKPPQHQVFI NFRGADLRRR FVSHLVTALK LNNINVFIDD YEDRGQPLDV
LLKRIEESKI VLAIFSGNYT ESVWCVRELE KIKDCTDEGT LVAIPIFYKL EPSTVRDLKG
KFGDRFRSMA KGDERKKKWK EAFNLIPNIM GIIIDKKSVE SEKVNEIVKA VKTALTGIPP
EGSHNAVVGA LGNSDAGTSS GDKKHETFGN EQRLKDLEEK LDRDKYKGTR IIGVVGMPGI
GKTTLLKELY KTWQGKFSRH ALIDQIRVKS KHLELDRLPQ MLLGELSKLN NPHVDNLKDP
YSQLHERKVL VVLDDVSKRE QIDALREILD WIKEGKEGSR VVIATSDMSL TNGLVDDTYM
VQNLNHRDSL QLFHYHAFID DQANPQKKDF MKLSEGFVHY ARGHPLALKV LGGELNKKSM
DHWNSKMKKL AQSPSPNIVS VFQVSYDELT TAQKDAFLDI ACFRSQDKDY VESLLASSDL
GSAEAMSAVK SLTDKFLINT CDGRVEMHDL LYKFSREVDL KASNQDGSRQ RRLWLHQHII
KGGIINVLQN KMKAANVRGI FLDLSEVEDE TSLDRDHFIN MGNLRYLKFY NSHCPQECKT
NNKINIPDKL KLPLKEVRCL HWLKFPLETL PNDFNPINLV DLKLPYSEME QLWEGDKDTP
CLRWVDLNHS SKLCSLSGLS KAEKLQRLNL EGCTTLKAFP HDMKKMKMLA FLNLKGCTSL
ESLPEMNLIS LKTLTLSGCS TFKEFPLISD NIETLYLDGT AISQLPMNME KLQRLVVLNM
KDCKMLEEIP GRVGELKALQ ELILSDCLNL KIFPEIDISF LNILLLDGTA IEVMPQLPSV
QYLCLSRNAK ISCLPVGISQ LSQLKWLDLK YCTSLTSVPE FPPNLQCLDA HGCSSLKTVS
KPLARIMPTE QNHSTFIFTN CENLEQAAKE EITSYAQRKC QLLSYARKRH NGGLVSESLF
STCFPGCEVP SWFCHETVGS ELEVKLLPHW HDKKLAGIAL CAVVSCLDPQ DQVSRLSVTC
TFKVKDEDKS WVPYTCPVGS WTRHGGGKDK IELDHVFIGY TSCPHTIKCH EEGNSDECNP
TEASLKFTVT GGTSENGKYK VLKCGLSLVY AKDKDKNSAL ETKYDMLIGK SFQETSEGVD
GRVKKTKGKY VMPVEKNFQE TTEGVDGRVK KKKKTRMDNG RPKKKQRSGR DDNQTRMQVE
LQEGNINSVI MHTVKNF
