RPS4W_ARATH
ID RPS4W_ARATH Reviewed; 1217 AA.
AC C4B7M7;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Disease resistance protein RPS4 {ECO:0000303|PubMed:19519800};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Resistance to Pseudomonas syringae 4 {ECO:0000303|PubMed:19519800};
GN Name=RPS4 {ECO:0000303|PubMed:19519800};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAH59426.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=19519800; DOI=10.1111/j.1365-313x.2009.03949.x;
RA Narusaka M., Shirasu K., Noutoshi Y., Kubo Y., Shiraishi T., Iwabuchi M.,
RA Narusaka Y.;
RT "RRS1 and RPS4 provide a dual Resistance-gene system against fungal and
RT bacterial pathogens.";
RL Plant J. 60:218-226(2009).
RN [2]
RP FUNCTION, ALTERNATIVE SPLICING, AND INDUCTION BY AVRRPS4.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
RX PubMed=17951452; DOI=10.1104/pp.107.108720;
RA Zhang X.C., Gassmann W.;
RT "Alternative splicing and mRNA levels of the disease resistance gene RPS4
RT are induced during defense responses.";
RL Plant Physiol. 145:1577-1587(2007).
RN [3]
RP FUNCTION.
RX PubMed=19826224; DOI=10.4161/psb.4.10.9640;
RA Narusaka M., Kubo Y., Shiraishi T., Iwabuchi M., Narusaka Y.;
RT "A dual resistance gene system prevents infection by three distinct
RT pathogens.";
RL Plant Signal. Behav. 4:954-955(2009).
RN [4]
RP FUNCTION.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=24146667; DOI=10.3389/fpls.2013.00403;
RA Heidrich K., Tsuda K., Blanvillain-Baufume S., Wirthmueller L., Bautor J.,
RA Parker J.E.;
RT "Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-
RT conditioned RPS4 auto-immunity.";
RL Front. Plant Sci. 4:403-403(2013).
CC -!- FUNCTION: Disease resistance (R) protein that specifically recognizes
CC the AvrRps4 type III effector avirulence protein from Pseudomonas
CC syringae. Resistance proteins guard the plant against pathogens that
CC contain an appropriate avirulence protein via an indirect interaction
CC with this avirulence protein. That triggers a defense system including
CC the hypersensitive response, which restricts the pathogen growth. The
CC combined presence of both regular and alternative RPS4 transcripts with
CC truncated open reading frames (ORFs) is necessary for function. RPS4
CC function is regulated at multiple levels, including gene expression,
CC alternative splicing, and protein stability. Acts as a disease
CC resistance protein involved in resistance to fungal and bacterial
CC pathogens, including R.solanacearum, P.syringae pv. tomato and
CC C.higginsianum. In presence of RRS1, elicites an EDS1-dependent
CC hypersensitive response (PubMed:24146667).
CC {ECO:0000269|PubMed:17951452, ECO:0000269|PubMed:19519800,
CC ECO:0000269|PubMed:19826224, ECO:0000269|PubMed:24146667}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- SUBUNIT: Interacts with EDS1. {ECO:0000250|UniProtKB:Q9XGM3}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced.
CC {ECO:0000269|PubMed:17951452};
CC Name=1;
CC IsoId=C4B7M7-1; Sequence=Displayed;
CC -!- INDUCTION: Up-regulated by AvrRps4 in an EDS1-dependent manner.
CC {ECO:0000269|PubMed:17951452}.
CC -!- DOMAIN: The TIR domain is a signaling domain involved in cell death
CC induction. {ECO:0000250|UniProtKB:Q9XGM3}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- DISRUPTION PHENOTYPE: Loss of resistance to C.higginsianum.
CC {ECO:0000269|PubMed:19519800}.
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DR EMBL; AB470473; BAH59426.1; -; mRNA.
DR AlphaFoldDB; C4B7M7; -.
DR SMR; C4B7M7; -.
DR ExpressionAtlas; C4B7M7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR045344; C-JID.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF20160; C-JID; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Leucine-rich repeat; NAD; Nucleus;
KW Plant defense; Repeat.
FT CHAIN 1..1217
FT /note="Disease resistance protein RPS4"
FT /id="PRO_0000431366"
FT DOMAIN 14..175
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 211..472
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 260..285
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 436..459
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 614..636
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 637..659
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 682..706
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 708..728
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 729..749
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 750..774
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 796..818
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 819..842
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 861..887
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REGION 1162..1195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1170..1177
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT ACT_SITE 88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
SQ SEQUENCE 1217 AA; 137745 MW; 9CAB2C913968EBA9 CRC64;
METSSISTVE DKPPQHQVFI NFRGADLRRR FVSHLVTALK LNNINVFIDD YEDRGQPLDV
LLKRIEESKI VLAIFSGNYT ESVWCVRELE KIKDCTDEGT LVAIPIFYKL EPSTVRDLKG
KFGDRFRSMA KGDERKKKWK EAFNLIPNIM GITIDKKSVE SEKVNEIVKA VKTALTGIPP
EGSHNAVVGA LGNSNAGTSS GDKKHETFGN EQRLKDLEEK LDRDKYKGTR IIGVVGMPGI
GKTTLLKELY KTWQGKFSRH ALIDQIRVKS KHLELDRLPQ MLLGELSKLN NPHVDNLKDP
YSQLHERKVL VVLDDVSKRE QIDALREILD WIKEGKEGSR VVIATSDMSL TNGLVDDTYM
VQNLNHRDSL QLFHYHAFID DQANPQKKDF MKLSEGFVHY ARGHPLALKV LGGELNKKSM
DHWNSKMKKL AQSPSPNIVS VFQVSYDELT TAQKDAFLDI ACFRSQDKDY VESLLASSDL
GSAEAMSAVK SLTDKFLINT CDGRVEMHDL LYKFSREIDL KASNQDGSRQ RRLWLHQHII
KGGIINVLQN KMKAANVRGI FLDLSEVEDE TSLDRDHFIN MGNLRYLKFY NSHCPQECKT
NNKINIPDKL KLPLKEVRCL HWLKFPLETL PNDFNPINLV DLKLPYSEME QLWEGDKDTP
CLRWVDLNHS SKLCSLSGLS KAEKLQRLNL EGCTTLKAFP HDMKKMKMLA FLNLKGCTSL
ESLPEMNLIS LKTLTLSGCS TFKEFPLISD NIETLYLDGT AISQLPMNME KLQRLVVLNM
KDCKMLEEIP GRVGELKALQ ELILSDCLNL KIFPEIDISF LNILLLDGTA IEVMPQLPSV
QYLCLSRNAK ISCLPVGISQ LSQLKWLDLK YCTSLTSVPE FPPNLQCLDA HGCSSLKTVS
KPLARIMPTE QNHSTFIFTN CENLEQAAKE EITSYAQRKC QLLSYARKRY NGGLVSESLF
STCFPGCEVP SWFCHETVGS ELEVKLLPHW HDKKLAGIAL CAVVSCLDPQ DQVSRLSVTC
TFKVKDEDKS WVPYTCPVGS WTRHGGGKDK IELDHVFIGY TSCPHTIKCH EEGNSDECNP
TEASLKFTVT GGTSENGKYK VLKCGLSLVY AKDKDKNSAL ETKYDMLIGK SFQETSEGVD
GRVKKTKGKY VMPVEKNFQE TTEGVDGRVK KKKKTRMDNG RPKKKQRSGR DDNQTRMQVE
LQEGNINSVI MHTVKNF
