ID   RPSB_CORDI              Reviewed;         329 AA.
AC   P33118;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2003, sequence version 2.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=RNA polymerase sigma factor SigB;
GN   Name=sigB; OrderedLocusNames=DIP1413;
OS   Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype
OS   gravis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=257309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis;
RX   PubMed=14602910; DOI=10.1093/nar/gkg874;
RA   Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G.,
RA   Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D.,
RA   De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N.,
RA   Holroyd S., Jagels K., Moule S., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G.,
RA   Parkhill J.;
RT   "The complete genome sequence and analysis of Corynebacterium diphtheriae
RT   NCTC13129.";
RL   Nucleic Acids Res. 31:6516-6523(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-329.
RC   STRAIN=C7(-);
RX   PubMed=2116013; DOI=10.1073/pnas.87.15.5968;
RA   Boyd J.M., Oza M.N., Murphy J.R.;
RT   "Molecular cloning and DNA sequence analysis of a diphtheria tox iron-
RT   dependent regulatory element (dtxR) from Corynebacterium diphtheriae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:5968-5972(1990).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA23295.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAA23298.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BX248358; CAE49944.1; -; Genomic_DNA.
DR   EMBL; M80338; AAA23298.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M34239; AAA23295.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003851800.1; NC_002935.2.
DR   AlphaFoldDB; P33118; -.
DR   SMR; P33118; -.
DR   STRING; 257309.DIP1413; -.
DR   EnsemblBacteria; CAE49944; CAE49944; DIP1413.
DR   GeneID; 29421138; -.
DR   KEGG; cdi:DIP1413; -.
DR   HOGENOM; CLU_014793_3_5_11; -.
DR   OMA; YQRWLAE; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000002198; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..329
FT                   /note="RNA polymerase sigma factor SigB"
FT                   /id="PRO_0000093940"
FT   DNA_BIND        290..309
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           120..133
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   329 AA;  37350 MW;  297D7CCDF7352020 CRC64;
     MTSPSDVEAS TETVDRGSRR NQTNDNPSAD LVRVYLNGIG KTALLNAEDE VELAQTIEVG
     LYAEYLLENS QEPLTRAMKR DLKVLVKEGR KARSHLLEAN LRLVVSLAKR YTGRGMPLLD
     LIQEGNLGLI RAMEKFDYSK GFKFSTYATW WIRQAITRGM ADQSRTIRLP VHLVEQVNKL
     SRIKREMYQQ LGREATNEEL AEESGIEESK IEMLLRQSRD PVSLDMPVGA DEEAPLGDFI
     EDSEATDAES AVVASLRHSD IRTVLDTLEQ REQDVIRLRY GLDDGVPRTL DQIGRQFGLS
     RERVRQIERE VMSKLRDGAR AEKLRAYAQ