ID   RPSB_NOSS1              Reviewed;         332 AA.
AC   Q03065;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=RNA polymerase sigma-B factor;
GN   Name=sigB; OrderedLocusNames=all7615;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OG   Plasmid pCC7120alpha.
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1385387; DOI=10.1128/jb.174.22.7273-7282.1992;
RA   Brahamsha B., Haselkorn R.;
RT   "Identification of multiple RNA polymerase sigma factor homologs in the
RT   cyanobacterium Anabaena sp. strain PCC 7120: cloning, expression, and
RT   inactivation of the sigB and sigC genes.";
RL   J. Bacteriol. 174:7273-7282(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; M95760; AAA22046.1; -; Genomic_DNA.
DR   EMBL; AP003602; BAB77258.1; -; Genomic_DNA.
DR   PIR; AD2541; AD2541.
DR   RefSeq; WP_010993943.1; NZ_RSCN01000012.1.
DR   AlphaFoldDB; Q03065; -.
DR   SMR; Q03065; -.
DR   EnsemblBacteria; BAB77258; BAB77258; BAB77258.
DR   KEGG; ana:all7615; -.
DR   OMA; RQLMADQ; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000002483; Plasmid pCC7120beta.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR017848; RNA_pol_sigma_RpoD_cyanobac.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00140; Sigma70_r1_2; 1.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02997; Sig70-cyanoRpoD; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..332
FT                   /note="RNA polymerase sigma-B factor"
FT                   /id="PRO_0000093935"
FT   DNA_BIND        294..313
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           125..138
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   332 AA;  38432 MW;  81323E670CCDF46A CRC64;
     MPNSTSQVTK LKSKNNEFSY TADAVSIYLH KIGRVPLLSH EQEIFFAQQV QQMMVMFTAK
     EELAEKLQRE PTLQEWADKM QLKEDVLLQQ LSQGQIAKQK MIQANLRLVV SIAKKYQKRN
     LEFLDLIQEG ALGLERGVEK FDPTLGYKFS TYAYWWIRQG ITRAIAQQSR TIRLPIHMAD
     KLNKIKCVQR ELSQKLGYIA GVTEIAQALN LEPSQIREYL QLVRQPVSLD MRIGFEQDTQ
     LQDLLKDDGM SPERYAEREL LYQDIHNLLA KLTPQQKEVL ILRFGLAGGC ELTLVQISQR
     MGISRERVRQ VEKQALTLLR RYGIDSRSYL AD