RPSC_MYCTO
ID RPSC_MYCTO Reviewed; 185 AA.
AC P9WGH0; L0T8P5; P66809; Q10679;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 40.
DE RecName: Full=ECF RNA polymerase sigma factor SigC;
DE Short=ECF sigma factor SigC;
DE AltName: Full=Alternative RNA polymerase sigma factor SigC;
DE AltName: Full=RNA polymerase sigma-C factor;
DE Short=Sigma-C factor;
GN Name=sigC; OrderedLocusNames=MT2129;
OS Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83331;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT laboratory strains.";
RL J. Bacteriol. 184:5479-5490(2002).
RN [2]
RP FUNCTION AS A SIGMA FACTOR, INDUCTION, BIOTECHNOLOGY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=15049808; DOI=10.1111/j.1365-2958.2003.03958.x;
RA Sun R., Converse P.J., Ko C., Tyagi S., Morrison N.E., Bishai W.R.;
RT "Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality
RT in mice and for the conditional expression of a defined gene set.";
RL Mol. Microbiol. 52:25-38(2004).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. Extracytoplasmic function (ECF) sigma factors are usually
CC held in an inactive form by an anti-sigma factor until released by
CC regulated intramembrane proteolysis; this sigma factor does not seem to
CC have a cognate anti-sigma factor however. It has been suggested that
CC the sigma domains 2 and 4 may interact via polar residues in this
CC protein to autoregulate. Positively regulates expression of a small
CC regulon of genes (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:15049808}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core
CC formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1
CC omega subunit) to form the RNA polymerase holoenzyme that can initiate
CC transcription. {ECO:0000250}.
CC -!- INDUCTION: The most abundant of the sigma factor transcripts, it is
CC expressed in exponential phase; repressed by detergent (7-fold), heat
CC shock (9-fold, 45 degrees Celsius) and in stationary phase.
CC Autoregulated (PubMed:15049808). {ECO:0000269|PubMed:15049808}.
CC -!- DOMAIN: The sigma-70 factor domain-2 mediates sequence-specific
CC interaction with the -10 element in promoter DNA, and plays an
CC important role in melting the double-stranded DNA and the formation of
CC the transcription bubble. The sigma-70 factor domain-2 mediates
CC interaction with the RNA polymerase subunits RpoB and RpoC (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-
CC H) motif that mediates interaction with the -35 element in promoter
CC DNA. The domain also mediates interaction with the RNA polymerase
CC subunit RpoA (By similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: No effect on growth in culture, or in isolated
CC macrophages. Essential for mouse lethality; infected DBA/2 mice survive
CC more than 3 times longer following infection with mutant bacterium
CC despite a similar bacterial burden. Disruption leads to repression of a
CC number of genes including itself and several virulence-associated
CC genes. {ECO:0000269|PubMed:15049808}.
CC -!- BIOTECHNOLOGY: The disruption mutant may be a good live attenuated
CC vaccine candidate because of its persistent, non-lethal pattern of
CC infection in mice. {ECO:0000269|PubMed:15049808}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK46409.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE000516; AAK46409.1; ALT_INIT; Genomic_DNA.
DR PIR; H70764; H70764.
DR RefSeq; WP_003410678.1; NZ_KK341227.1.
DR AlphaFoldDB; P9WGH0; -.
DR SMR; P9WGH0; -.
DR EnsemblBacteria; AAK46409; AAK46409; MT2129.
DR KEGG; mtc:MT2129; -.
DR PATRIC; fig|83331.31.peg.2297; -.
DR HOGENOM; CLU_893774_0_0_11; -.
DR Proteomes; UP000001020; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009628; P:response to abiotic stimulus; IEA:UniProt.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS01063; SIGMA70_ECF; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Sigma factor; Transcription; Transcription regulation.
FT CHAIN 1..185
FT /note="ECF RNA polymerase sigma factor SigC"
FT /id="PRO_0000428363"
FT DNA_BIND 149..168
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 30..94
FT /note="Sigma-70 factor domain-2"
FT REGION 123..174
FT /note="Sigma-70 factor domain-4"
FT MOTIF 52..55
FT /note="Polymerase core binding"
FT /evidence="ECO:0000255"
SQ SEQUENCE 185 AA; 19966 MW; FF839B4279929917 CRC64;
MTATASDDEA VTALALSAAK GNGRALEAFI KATQQDVWRF VAYLSDVGSA DDLTQETFLR
AIGAIPRFSA RSSARTWLLA IARHVVADHI RHVRSRPRTT RGARPEHLID GDRHARGFED
LVEVTTMIAD LTTDQREALL LTQLLGLSYA DAAAVCGCPV GTIRSRVARA RDALLADAEP
DDLTG
