RPSE_BACSU
ID RPSE_BACSU Reviewed; 239 AA.
AC P06222; P15810; Q59252;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=RNA polymerase sigma-E factor;
DE AltName: Full=P31;
DE AltName: Full=Sigma-29;
DE AltName: Full=Stage II sporulation protein GB;
DE Flags: Precursor;
GN Name=sigE; Synonyms=spoIIGB; OrderedLocusNames=BSU15320;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6438529; DOI=10.1038/312376a0;
RA Stragier P., Bouvier J., Bonamy C., Szulmajster J.;
RT "A developmental gene product of Bacillus subtilis homologous to the sigma
RT factor of Escherichia coli.";
RL Nature 312:376-378(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kobayashi Y., Anaguchi H.;
RT "Cloning, amplification, and characterization of sporulation genes,
RT especially spoIIG, of Bacillus subtilis.";
RL (In) Setlow P. (eds.);
RL Molecular biology of microbial differentiation, pp.85-94, American Society
RL for Microbiology, Washington D.C. (1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 66 AND 139.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP IDENTIFICATION OF MATURE SIGMA FACTOR, AND PROTEIN SEQUENCE OF 30-36.
RX PubMed=3104904; DOI=10.1073/pnas.84.7.1784;
RA Labell T.L., Trempy J.E., Haldenwang W.G.;
RT "Sporulation-specific sigma factor sigma 29 of Bacillus subtilis is
RT synthesized from a precursor protein, P31.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:1784-1788(1987).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RC STRAIN=168;
RX PubMed=2106671; DOI=10.1093/nar/18.3.657;
RA Masuda E.S., Anaguchi H., Sato T., Takeuchi M., Kobayashi Y.;
RT "Nucleotide sequence of the sporulation gene spoIIGA from Bacillus
RT subtilis.";
RL Nucleic Acids Res. 18:657-657(1990).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of
CC sporulation specific genes.
CC -!- PTM: Proteolytically cleaved in the N-terminus by SpoIIGA to yield the
CC active peptide.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; X01180; CAA25620.1; -; Genomic_DNA.
DR EMBL; M57606; AAA22794.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13406.2; -; Genomic_DNA.
DR EMBL; M15804; AAA72566.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X17344; CAA35226.1; -; Genomic_DNA.
DR PIR; S07337; JU0083.
DR RefSeq; NP_389415.2; NC_000964.3.
DR RefSeq; WP_003221446.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P06222; -.
DR SMR; P06222; -.
DR STRING; 224308.BSU15320; -.
DR PaxDb; P06222; -.
DR PRIDE; P06222; -.
DR EnsemblBacteria; CAB13406; CAB13406; BSU_15320.
DR GeneID; 64303423; -.
DR GeneID; 939683; -.
DR KEGG; bsu:BSU15320; -.
DR PATRIC; fig|224308.179.peg.1670; -.
DR eggNOG; COG1191; Bacteria.
DR InParanoid; P06222; -.
DR OMA; EPLNTDW; -.
DR PhylomeDB; P06222; -.
DR BioCyc; BSUB:BSU15320-MON; -.
DR PRO; PR:P06222; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014200; RNA_pol_sigma-E.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02835; spore_sigmaE; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-binding; Reference proteome; Sigma factor;
KW Sporulation; Transcription; Transcription regulation.
FT PROPEP 1..29
FT /note="Removed by SpoIIGA"
FT /evidence="ECO:0000269|PubMed:3104904"
FT /id="PRO_0000032580"
FT CHAIN 30..239
FT /note="RNA polymerase sigma-E factor"
FT /id="PRO_0000032581"
FT DNA_BIND 206..225
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 86..99
FT /note="Polymerase core binding"
FT CONFLICT 66
FT /note="N -> I (in Ref. 2; AAA22794)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="D -> Y (in Ref. 2; AAA22794)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 27652 MW; 6C5179566C4B4CC9 CRC64;
MKKLKLRLTH LWYKLLMKLG LKSDEVYYIG GSEALPPPLS KDEEQVLLMK LPNGDQAARA
ILIERNLRLV VYIARKFENT GINIEDLISI GTIGLIKAVN TFNPEKKIKL ATYASRCIEN
EILMYLRRNN KIRSEVSFDE PLNIDWDGNE LLLSDVLGTD DDIITKDIEA NVDKKLLKKA
LEQLNEREKQ IMELRFGLVG EEEKTQKDVA DMMGISQSYI SRLEKRIIKR LRKEFNKMV
