ID   RPSE_CLOAB              Reviewed;         235 AA.
AC   P33657;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   25-MAY-2022, entry version 132.
DE   RecName: Full=RNA polymerase sigma-E factor;
DE   AltName: Full=Stage II sporulation protein GB;
GN   Name=sigE; Synonyms=spoIIGB; OrderedLocusNames=CA_C1695;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=7883192; DOI=10.1016/0378-1119(94)00818-d;
RA   Wong J., Sass C., Bennett G.N.;
RT   "Sequence and arrangement of genes encoding sigma factors in Clostridium
RT   acetobutylicum ATCC 824.";
RL   Gene 153:89-92(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=9561744; DOI=10.1111/j.1574-6968.1998.tb12943.x;
RA   Santangelo J.D., Kuhn A., Treuner-Lange A., Durre P.;
RT   "Sporulation and time course expression of sigma-factor homologous genes in
RT   Clostridium acetobutylicum.";
RL   FEMS Microbiol. Lett. 161:157-164(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-235.
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=7961408; DOI=10.1128/jb.176.21.6572-6582.1994;
RA   Sauer U., Treuner A., Buchholz M., Santangelo J.D., Durre P.;
RT   "Sporulation and primary sigma factor homologous genes in Clostridium
RT   acetobutylicum.";
RL   J. Bacteriol. 176:6572-6582(1994).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of
CC       sporulation specific genes (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; U07420; AAC43309.1; -; Genomic_DNA.
DR   EMBL; Z23079; CAA80617.1; -; Genomic_DNA.
DR   EMBL; AE001437; AAK79661.1; -; Genomic_DNA.
DR   PIR; B97109; B97109.
DR   PIR; I40627; I40627.
DR   RefSeq; NP_348321.1; NC_003030.1.
DR   RefSeq; WP_010965002.1; NC_003030.1.
DR   AlphaFoldDB; P33657; -.
DR   SMR; P33657; -.
DR   STRING; 272562.CA_C1695; -.
DR   EnsemblBacteria; AAK79661; AAK79661; CA_C1695.
DR   GeneID; 44998190; -.
DR   KEGG; cac:CA_C1695; -.
DR   PATRIC; fig|272562.8.peg.1898; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_014793_8_7_9; -.
DR   OMA; EPLNTDW; -.
DR   OrthoDB; 1565734at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014200; RNA_pol_sigma-E.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02835; spore_sigmaE; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..235
FT                   /note="RNA polymerase sigma-E factor"
FT                   /id="PRO_0000093941"
FT   DNA_BIND        202..221
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           82..95
FT                   /note="Polymerase core binding"
FT   CONFLICT        147
FT                   /note="L -> P (in Ref. 4; CAA80617)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   235 AA;  26970 MW;  C726E18E6C93A903 CRC64;
     MKFLLRLLSK LLCRIKLFSN RLYYIGGNDA LPPPLSKDEE DDLVNKLVNG DESIRSILIE
     RNLRLVVYIA RKFENTGVGV EDLISVGTIG LIKAVNTFDP TKKIKLATYG SRCIENEILM
     YLRRNSKVKA EISFYEPLNI DWDGNKLLLS DILGTDNDCV YNLIEGEVDK QLLLFALKKL
     NEREKRIVEL RYGLTGVGEK TQKEVADMLG ISQSYISRLE KRIIKRLKKE INKML