ID   RPSF_PRIMG              Reviewed;         253 AA.
AC   P35145;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=RNA polymerase sigma-F factor;
DE   AltName: Full=Sporulation sigma factor;
DE   AltName: Full=Stage II sporulation protein AC;
GN   Name=sigF; Synonyms=spoIIAC;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1391049; DOI=10.1016/0300-9084(92)90142-2;
RA   Tao Y.P., Hudspeth D.S.S., Vary P.S.;
RT   "Cloning and sequencing of the Bacillus megaterium spoIIA operon.";
RL   Biochimie 74:695-704(1992).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of
CC       sporulation specific genes. It is responsible for directing gene
CC       expression in the forespore compartment of developing cells of
CC       Bacillus.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; X63757; CAA45288.1; -; Genomic_DNA.
DR   PIR; C48402; C48402.
DR   RefSeq; WP_013059074.1; NZ_WIPB01000105.1.
DR   AlphaFoldDB; P35145; -.
DR   SMR; P35145; -.
DR   GeneID; 48014867; -.
DR   GeneID; 64145904; -.
DR   OMA; RDDGMIK; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014322; RNA_pol_sigma-B/F/G.
DR   InterPro; IPR014236; RNA_pol_sigma-F.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02980; SigBFG; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02885; spore_sigF; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..253
FT                   /note="RNA polymerase sigma-F factor"
FT                   /id="PRO_0000093942"
FT   DNA_BIND        221..240
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           61..74
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   253 AA;  29247 MW;  AB267B47004276BD CRC64;
     MDVEVKKNKN EPYLKDHEVK DLIKRSQQGD QIARDTIVQK NMRLVWSVVQ RFINRGYEPD
     DLFQIGCIGL LKSVDKFDLS YDVKFSTYAV PMIIGEIQRF IRDDGTVKVS RSLKEMSNKI
     RKAKDELSKL LGRVPTVAEV AEHLDLTPEE VVLAQEANRA PSSIHETVYE NDGDPITLLD
     QIADHTEAKW FDKIALKEAI EELDEREKLI VYLRYYKDQT QSEVAARLGI SQVQVSRLEK
     KILKQMKLHM NDT