ID   RPSG_BACSU              Reviewed;         260 AA.
AC   P19940;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=RNA polymerase sigma-G factor;
DE   AltName: Full=Stage III sporulation protein G;
GN   Name=sigG; Synonyms=spoIIIG; OrderedLocusNames=BSU15330;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2497052; DOI=10.1101/gad.3.2.150;
RA   Karmazyn-Campelli C., Bonamy C., Savelli B., Stragier P.;
RT   "Tandem genes encoding sigma-factors for consecutive steps of development
RT   in Bacillus subtilis.";
RL   Genes Dev. 3:150-157(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2459711; DOI=10.1073/pnas.85.20.7637;
RA   Masuda E.S., Anaguchi H., Yamada K., Kobayashi Y.;
RT   "Two developmental genes encoding sigma factor homologs are arranged in
RT   tandem in Bacillus subtilis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:7637-7641(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   ACTIVITY REGULATION.
RC   STRAIN=168 / BR151;
RX   PubMed=17921305; DOI=10.1128/jb.01265-07;
RA   Chary V.K., Xenopoulos P., Piggot P.J.;
RT   "Expression of the sigmaF-directed csfB locus prevents premature appearance
RT   of sigmaG activity during sporulation of Bacillus subtilis.";
RL   J. Bacteriol. 189:8754-8757(2007).
RN   [5]
RP   FUNCTION, INTERACTION WITH GIN, DEVELOPMENTAL STAGE, INDUCTION, AND DOMAIN.
RC   STRAIN=168 / JH642;
RX   PubMed=18208527; DOI=10.1111/j.1365-2958.2008.06121.x;
RA   Karmazyn-Campelli C., Rhayat L., Carballido-Lopez R., Duperrier S.,
RA   Frandsen N., Stragier P.;
RT   "How the early sporulation sigma factor sigmaF delays the switch to late
RT   development in Bacillus subtilis.";
RL   Mol. Microbiol. 67:1169-1180(2008).
RN   [6]
RP   ACTIVITY REGULATION, AND INTERACTION WITH SIGMA-G FACTOR.
RX   PubMed=19497328; DOI=10.1016/j.jmb.2009.05.073;
RA   Rhayat L., Duperrier S., Carballido-Lopez R., Pellegrini O., Stragier P.;
RT   "Genetic dissection of an inhibitor of the sporulation sigma factor
RT   sigma(G).";
RL   J. Mol. Biol. 390:835-844(2009).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released (PubMed:18208527). This sigma factor is responsible for the
CC       expression of sporulation specific genes in the forespore
CC       (PubMed:18208527). {ECO:0000269|PubMed:18208527}.
CC   -!- ACTIVITY REGULATION: Activity repressed by anti-sigma-G factor Gin
CC       (csfB) and Lon protease during the early stages of forespore
CC       development (PubMed:17921305). When both Gin and sigma-G are expressed
CC       in E.coli Gin inhibits sigma-G activity, strongly suggesting Gin
CC       inhibits by direct physical interaction (PubMed:19497328).
CC       {ECO:0000269|PubMed:17921305, ECO:0000269|PubMed:19497328}.
CC   -!- SUBUNIT: Interacts with anti-sigma-G factor Gin (csfB)
CC       (PubMed:18208527, PubMed:19497328). {ECO:0000269|PubMed:18208527,
CC       ECO:0000269|PubMed:19497328}.
CC   -!- DEVELOPMENTAL STAGE: Active only in the forespore (PubMed:18208527).
CC       {ECO:0000269|PubMed:18208527}.
CC   -!- INDUCTION: Expressed and active 2 hours after sporulation starts (at
CC       protein level); stimulates its own transcription (PubMed:18208527).
CC       {ECO:0000269|PubMed:18208527}.
CC   -!- DOMAIN: Recognition of anti-sigma-G factor Gin (csfB) occurs via the
CC       first 71 residues (PubMed:18208527). {ECO:0000269|PubMed:18208527}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; X57547; CAA40771.1; -; Genomic_DNA.
DR   EMBL; J04077; AAA22703.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13407.1; -; Genomic_DNA.
DR   PIR; A30171; A30202.
DR   RefSeq; NP_389416.1; NC_000964.3.
DR   RefSeq; WP_009967190.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P19940; -.
DR   SMR; P19940; -.
DR   STRING; 224308.BSU15330; -.
DR   PaxDb; P19940; -.
DR   PRIDE; P19940; -.
DR   EnsemblBacteria; CAB13407; CAB13407; BSU_15330.
DR   GeneID; 939953; -.
DR   KEGG; bsu:BSU15330; -.
DR   PATRIC; fig|224308.179.peg.1671; -.
DR   eggNOG; COG1191; Bacteria.
DR   InParanoid; P19940; -.
DR   OMA; CGVNTAK; -.
DR   PhylomeDB; P19940; -.
DR   BioCyc; BSUB:BSU15330-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014322; RNA_pol_sigma-B/F/G.
DR   InterPro; IPR014212; RNA_pol_sigma-G.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007624; RNA_pol_sigma70_r3.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04539; Sigma70_r3; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02980; SigBFG; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02850; spore_sigG; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..260
FT                   /note="RNA polymerase sigma-G factor"
FT                   /id="PRO_0000093945"
FT   DNA_BIND        229..248
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          1..71
FT                   /note="Recognizes anti-sigma-G factor Gin (csfB)"
FT                   /evidence="ECO:0000269|PubMed:18208527"
FT   MOTIF           67..80
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   260 AA;  30073 MW;  FC4040ED31EB47D2 CRC64;
     MSRNKVEICG VDTSKLPVLK NEEMRKLFRQ LQDEGDDSAR EKLVNGNLRL VLSVIQRFNN
     RGEYVDDLFQ VGCIGLMKSI DNFDLSHNVK FSTYAVPMII GEIRRYLRDN NPIRVSRSLR
     DIAYKALQVR ERLISETSKE PTAEDIAKVL EVPHEEIVFA LDAIQDPVSL FEPIYNDGGD
     PIYVMDQISD ERNTDSQWIE ELALKEGMRR LNDREKMILR KRFFQGKTQM EVAEEIGISQ
     AQVSRLEKAA IKQMNKNIHQ