RPSH_BACSU
ID RPSH_BACSU Reviewed; 218 AA.
AC P17869;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=RNA polymerase sigma-H factor;
DE AltName: Full=Sigma-30;
DE AltName: Full=Stage 0 sporulation protein H;
GN Name=sigH; Synonyms=spo0H; OrderedLocusNames=BSU00980;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3277943; DOI=10.1128/jb.170.3.1054-1062.1988;
RA Dubnau E., Weir J., Nair G., Carter L. III, Moran C.P. Jr., Smith I.;
RT "Bacillus sporulation gene spo0H codes for sigma 30 (sigma H).";
RL J. Bacteriol. 170:1054-1062(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-218.
RX PubMed=7968510; DOI=10.1111/j.1365-2958.1993.tb00910.x;
RA Jeong S., Yoshikawa H., Takahashi H.;
RT "Isolation and characterization of the secE homologue gene of Bacillus
RT subtilis.";
RL Mol. Microbiol. 10:133-142(1993).
RN [4]
RP MUTAGENESIS OF THR-100.
RC STRAIN=168 / JH642;
RX PubMed=2500529; DOI=10.1016/0022-2836(89)90569-x;
RA Zuber P., Healy J., Carter H.L. III, Cutting S., Moran C.P. Jr., Losick R.;
RT "Mutation changing the specificity of an RNA polymerase sigma factor.";
RL J. Mol. Biol. 206:605-614(1989).
RN [5]
RP MUTAGENESIS OF ARG-96 AND THR-100.
RC STRAIN=168 / JH642;
RX PubMed=2122453; DOI=10.1073/pnas.87.20.8075;
RA Daniels D., Zuber P., Losick R.;
RT "Two amino acids in an RNA polymerase sigma factor involved in the
RT recognition of adjacent base pairs in the -10 region of a cognate
RT promoter.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:8075-8079(1990).
RN [6]
RP FUNCTION, SUBUNIT, AND INDUCTION BY SPORULATION.
RC STRAIN=168;
RX PubMed=21710567; DOI=10.1002/pmic.201000790;
RA Delumeau O., Lecointe F., Muntel J., Guillot A., Guedon E., Monnet V.,
RA Hecker M., Becher D., Polard P., Noirot P.;
RT "The dynamic protein partnership of RNA polymerase in Bacillus subtilis.";
RL Proteomics 11:2992-3001(2011).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase (RNAP) to specific initiation sites and
CC are then released. This sigma factor is involved in the transition to
CC post-exponential phase in the beginning of sporulation. It is also
CC required for transcription of several stationary phase genes.
CC Association with the RNAP core increases rapidly in early exponential
CC phase, and reamins constant expression level after (PubMed:21710567).
CC {ECO:0000269|PubMed:21710567}.
CC -!- SUBUNIT: Interacts transiently with the RNAP core.
CC {ECO:0000305|PubMed:21710567}.
CC -!- INDUCTION: Association with RNAP core increases during sporulation but
CC not tested stresses (at protein level). {ECO:0000269|PubMed:21710567}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR EMBL; M29693; AAA22756.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11874.1; -; Genomic_DNA.
DR EMBL; D13303; BAA02557.1; -; Genomic_DNA.
DR PIR; A28625; A28625.
DR RefSeq; NP_387979.1; NC_000964.3.
DR RefSeq; WP_009966312.1; NZ_JNCM01000029.1.
DR AlphaFoldDB; P17869; -.
DR SMR; P17869; -.
DR STRING; 224308.BSU00980; -.
DR PaxDb; P17869; -.
DR PRIDE; P17869; -.
DR DNASU; 936150; -.
DR EnsemblBacteria; CAB11874; CAB11874; BSU_00980.
DR GeneID; 936150; -.
DR KEGG; bsu:BSU00980; -.
DR PATRIC; fig|224308.179.peg.101; -.
DR eggNOG; COG1595; Bacteria.
DR InParanoid; P17869; -.
DR OMA; LEWKVLM; -.
DR PhylomeDB; P17869; -.
DR BioCyc; BSUB:BSU00980-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProt.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014218; RNA_pol_sigma-H.
DR InterPro; IPR016371; RNA_pol_sigma-H_factor.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR PIRSF; PIRSF002939; RNA_polymerase_sigma-H_factor; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02859; spore_sigH; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW Transcription regulation.
FT CHAIN 1..218
FT /note="RNA polymerase sigma-H factor"
FT /id="PRO_0000093949"
FT DNA_BIND 182..201
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 62..75
FT /note="Polymerase core binding"
FT MUTAGEN 96
FT /note="R->A: Suppressor of mutations in the -12 region of
FT the spoVG promoter."
FT /evidence="ECO:0000269|PubMed:2122453"
FT MUTAGEN 100
FT /note="T->A: Suppressor of mutations in the -13 region of
FT the spoVG promoter."
FT /evidence="ECO:0000269|PubMed:2122453,
FT ECO:0000269|PubMed:2500529"
FT MUTAGEN 100
FT /note="T->I: Loss of ability to recognize wild-type spoVG
FT promoter. Suppressor of the spoVG249 mutation in the -10
FT region of the spoVG promoter."
FT /evidence="ECO:0000269|PubMed:2122453,
FT ECO:0000269|PubMed:2500529"
SQ SEQUENCE 218 AA; 25447 MW; 7310E3FF153D99C1 CRC64;
MNLQNNKGKF NKEQFCQLED EQVIEKVHVG DSDALDYLIT KYRNFVRAKA RSYFLIGADR
EDIVQEGMIG LYKSIRDFKE DKLTSFKAFA ELCITRQIIT AIKTATRQKH IPLNSYASLD
KPIFDEESDR TLLDVISGAK TLNPEEMIIN QEEFDDIEMK MGELLSDLER KVLVLYLDGR
SYQEISDELN RHVKSIDNAL QRVKRKLEKY LEIREISL
