RPSH_PSEAE
ID RPSH_PSEAE Reviewed; 193 AA.
AC Q06198; Q6LCW4;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=RNA polymerase sigma-H factor;
DE AltName: Full=Sigma-30;
GN Name=algU; Synonyms=algT; OrderedLocusNames=PA0762;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8432708; DOI=10.1128/jb.175.4.1153-1164.1993;
RA Martin D.W., Holloway B.W., Deretic V.;
RT "Characterization of a locus determining the mucoid status of Pseudomonas
RT aeruginosa: AlgU shows sequence similarities with a Bacillus sigma
RT factor.";
RL J. Bacteriol. 175:1153-1164(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO381, and PAO568;
RX PubMed=8378309; DOI=10.1073/pnas.90.18.8377;
RA Martin D.W., Schurr M.J., Mudd M.H., Govan J.R.W., Holloway B.W.,
RA Deretic V.;
RT "Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting
RT cystic fibrosis patients.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:8377-8381(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FRD1;
RX PubMed=7961421; DOI=10.1128/jb.176.21.6677-6687.1994;
RA Devries C.A., Ohman D.E.;
RT "Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas
RT aeruginosa often results from spontaneous mutations in algT, encoding a
RT putative alternate sigma factor, and shows evidence for autoregulation.";
RL J. Bacteriol. 176:6677-6687(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
RC STRAIN=FRD1;
RX PubMed=7737518; DOI=10.1016/0378-1119(95)00028-5;
RA DeVries C.A., Hassett D.J., Flynn J.L., Ohman D.E.;
RT "Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in
RT nadB does not affect NAD biosynthesis or alginate production.";
RL Gene 156:63-67(1995).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor regulates genes such as algD, involved in
CC alginate biosynthesis.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
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DR EMBL; U49151; AAC43714.1; -; Genomic_DNA.
DR EMBL; L14760; AAA87628.1; -; Genomic_DNA.
DR EMBL; L14761; AAA87631.1; -; Genomic_DNA.
DR EMBL; L36379; AAA64439.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04151.1; -; Genomic_DNA.
DR EMBL; U17232; AAA92355.1; -; Genomic_DNA.
DR PIR; A49704; A49704.
DR RefSeq; NP_249453.1; NC_002516.2.
DR RefSeq; WP_003085543.1; NZ_QZGE01000007.1.
DR PDB; 6IN7; X-ray; 1.96 A; B=1-193.
DR PDBsum; 6IN7; -.
DR AlphaFoldDB; Q06198; -.
DR SMR; Q06198; -.
DR STRING; 287.DR97_1221; -.
DR PaxDb; Q06198; -.
DR PRIDE; Q06198; -.
DR DNASU; 882125; -.
DR EnsemblBacteria; AAG04151; AAG04151; PA0762.
DR GeneID; 882125; -.
DR KEGG; pae:PA0762; -.
DR PATRIC; fig|208964.12.peg.792; -.
DR PseudoCAP; PA0762; -.
DR HOGENOM; CLU_047691_3_0_6; -.
DR InParanoid; Q06198; -.
DR OMA; NAKAWLF; -.
DR PhylomeDB; Q06198; -.
DR BioCyc; PAER208964:G1FZ6-775-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0000345; C:cytosolic DNA-directed RNA polymerase complex; IDA:PseudoCAP.
DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR GO; GO:0016987; F:sigma factor activity; IDA:PseudoCAP.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0071236; P:cellular response to antibiotic; IMP:PseudoCAP.
DR GO; GO:0036460; P:cellular response to cell envelope stress; IDA:PseudoCAP.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:1902201; P:negative regulation of bacterial-type flagellum-dependent cell motility; IMP:PseudoCAP.
DR GO; GO:1900189; P:positive regulation of cell adhesion involved in single-species biofilm formation; IMP:PseudoCAP.
DR GO; GO:1900036; P:positive regulation of cellular response to heat; IDA:PseudoCAP.
DR GO; GO:1902884; P:positive regulation of response to oxidative stress; IMP:PseudoCAP.
DR GO; GO:1900233; P:positive regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEP:CollecTF.
DR GO; GO:0032885; P:regulation of polysaccharide biosynthetic process; IMP:PseudoCAP.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR039425; RNA_pol_sigma-70-like.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR014286; RNA_pol_sigma70_RpoE.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43133; PTHR43133; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02939; RpoE_Sigma70; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS01063; SIGMA70_ECF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alginate biosynthesis; DNA-binding; Reference proteome;
KW Sigma factor; Transcription; Transcription regulation.
FT CHAIN 1..193
FT /note="RNA polymerase sigma-H factor"
FT /id="PRO_0000094007"
FT DNA_BIND 157..176
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 49..62
FT /note="Polymerase core binding"
FT HELIX 8..14
FT /evidence="ECO:0007829|PDB:6IN7"
FT TURN 15..17
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 19..41
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 44..60
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 61..64
FT /evidence="ECO:0007829|PDB:6IN7"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 71..90
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 111..116
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 119..137
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 141..151
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 157..164
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 168..186
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:6IN7"
SQ SEQUENCE 193 AA; 22196 MW; 9C10419C51F29C64 CRC64;
MLTQEQDQQL VERVQRGDKR AFDLLVLKYQ HKILGLIVRF VHDAQEAQDV AQEAFIKAYR
ALGNFRGDSA FYTWLYRIAI NTAKNHLVAR GRRPPDSDVT AEDAEFFEGD HALKDIESPE
RAMLRDEIEA TVHQTIQQLP EDLRTALTLR EFEGLSYEDI ATVMQCPVGT VRSRIFRARE
AIDKALQPLL REA
