RR10_SPIOL
ID RR10_SPIOL Reviewed; 195 AA.
AC P82162; A0A0K9RWE7; P82161;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=30S ribosomal protein S10 alpha, chloroplastic {ECO:0000303|PubMed:10874039};
DE AltName: Full=Chloroplastic small ribosomal subunit protein uS10c {ECO:0000303|PubMed:28007896};
DE Contains:
DE RecName: Full=30S ribosomal protein S10 beta, chloroplastic {ECO:0000303|PubMed:10874039};
DE Flags: Precursor;
GN Name=RPS10; ORFNames=SOVF_026730;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [2]
RP PROTEIN SEQUENCE OF 60-81, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874039; DOI=10.1074/jbc.m004350200;
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 30S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28455-28465(2000).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [4]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874039,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast small ribosomal subunit (SSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874039,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874039, ECO:0000269|PubMed:28007896}.
CC -!- MISCELLANEOUS: 2 different forms exist, S10 alpha and S10 beta,
CC possibly due to different transit peptide cleavage.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS10 family.
CC {ECO:0000269|PubMed:10874039}.
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DR EMBL; KQ134529; KNA23234.1; -; Genomic_DNA.
DR PDB; 4V61; EM; 9.40 A; J=60-81.
DR PDB; 5MMJ; EM; 3.65 A; j=1-195.
DR PDB; 5MMM; EM; 3.40 A; j=1-195.
DR PDB; 5X8P; EM; 3.40 A; j=74-195.
DR PDB; 5X8R; EM; 3.70 A; j=74-195.
DR PDB; 6ERI; EM; 3.00 A; BJ=98-195.
DR PDBsum; 4V61; -.
DR PDBsum; 5MMJ; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8R; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P82162; -.
DR SMR; P82162; -.
DR STRING; 3562.P82162; -.
DR OrthoDB; 1528895at2759; -.
DR EvolutionaryTrace; P82162; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 3.30.70.600; -; 1.
DR HAMAP; MF_00508; Ribosomal_S10; 1.
DR InterPro; IPR001848; Ribosomal_S10.
DR InterPro; IPR018268; Ribosomal_S10_CS.
DR InterPro; IPR027486; Ribosomal_S10_dom.
DR InterPro; IPR036838; Ribosomal_S10_dom_sf.
DR PANTHER; PTHR11700; PTHR11700; 1.
DR Pfam; PF00338; Ribosomal_S10; 1.
DR PRINTS; PR00971; RIBOSOMALS10.
DR SMART; SM01403; Ribosomal_S10; 1.
DR SUPFAM; SSF54999; SSF54999; 1.
DR TIGRFAMs; TIGR01049; rpsJ_bact; 1.
DR PROSITE; PS00361; RIBOSOMAL_S10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT TRANSIT 1..59
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874039"
FT CHAIN 60..195
FT /note="30S ribosomal protein S10 alpha, chloroplastic"
FT /id="PRO_0000249417"
FT CHAIN 63..195
FT /note="30S ribosomal protein S10 beta, chloroplastic"
FT /id="PRO_0000249418"
SQ SEQUENCE 195 AA; 21414 MW; 123709684D99A4FC CRC64;
MATSSISAAL LSPLTLRNAS SSSTKQDFST LSSLNLRRTL TPTLQSGHTL SNSSNFATFA
APGALEVLET SPDSFEDGSE TSKISIAADS DQMAPKQKIR IKLRSYWVPL IEDSCKQIMD
AARTTNAKIM GPVPLPTKKR IYCVLKSPHV HKDARFHFEI RTHQRLIDIL YPTAQTIDSL
MQLDLPAGVD VEVKL
