ID   AUR52_RANAE             Reviewed;          71 AA.
AC   P69031; P82402; Q5K0E2;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Aurein-5.2 {ECO:0000303|PubMed:10951191};
DE   AltName: Full=Aurein-5.3 {ECO:0000303|PubMed:15721491};
DE   Flags: Precursor;
OS   Ranoidea aurea (Green and golden bell frog) (Litoria aurea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae; Ranoidea.
OX   NCBI_TaxID=8371;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin;
RX   PubMed=15721491; DOI=10.1016/j.regpep.2004.12.022;
RA   Chen T., Xue Y., Zhou M., Shaw C.;
RT   "The structural organization of aurein precursor cDNAs from the skin
RT   secretion of the Australian green and golden bell frog, Litoria aurea.";
RL   Regul. Pept. 128:75-83(2005).
RN   [2]
RP   PROTEIN SEQUENCE, FUNCTION, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=10951191; DOI=10.1046/j.1432-1327.2000.01536.x;
RA   Rozek T., Wegener K.L., Bowie J.H., Olver I.N., Carver J.A., Wallace J.C.,
RA   Tyler M.J.;
RT   "The antibiotic and anticancer active aurein peptides from the australian
RT   bell frogs Litoria aurea and Litoria raniformis the solution structure of
RT   aurein 1.2.";
RL   Eur. J. Biochem. 267:5330-5341(2000).
CC   -!- FUNCTION: Amphipathic alpha-helical antimicrobial peptide with moderate
CC       activity against Gram-positive bacteria (MIC=100 ug/mL against L.lactis
CC       and MIC=50 ug/mL against S.uberis) (PubMed:10951191). Has no activity
CC       against Gram-negative bacteria (PubMed:10951191).
CC       {ECO:0000269|PubMed:10951191}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10951191,
CC       ECO:0000269|PubMed:15721491}. Target cell membrane {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin dorsal glands.
CC       {ECO:0000269|PubMed:10951191, ECO:0000305|PubMed:15721491}.
CC   -!- MASS SPECTROMETRY: Mass=2452.99; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15721491};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Aurein subfamily. {ECO:0000305}.
CC   -!- CAUTION: Aurein 5.2 may be a deamidated form of aurein 5.3.
CC       {ECO:0000305|PubMed:15721491}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=02005";
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ850131; CAH61715.1; -; mRNA.
DR   AlphaFoldDB; P69031; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR032021; Frog_Litoria.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF16049; Antimicrobial24; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Membrane; Secreted; Signal;
KW   Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..46
FT                   /evidence="ECO:0000305|PubMed:15721491"
FT                   /id="PRO_0000450294"
FT   PEPTIDE         47..71
FT                   /note="Aurein-5.2"
FT                   /evidence="ECO:0000269|PubMed:10951191"
FT                   /id="PRO_0000043735"
FT   REGION          26..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        62
FT                   /note="D -> N (in Ref. 1; CAH61715)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   71 AA;  7825 MW;  C0A1971A3B83CBEC CRC64;
     MAFLKKSLFL VLFLGLVSLS ICEQEKREEE NQEEDEENEA ASEEKRGLMS SIGKALGGLI
     VDVLKPKTPA S