RR12_CHLRE
ID RR12_CHLRE Reviewed; 133 AA.
AC P14149; B7U1K0;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=30S ribosomal protein S12, chloroplastic;
GN Name=rps12;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND STREPTOMYCIN RESISTANT MUTANTS.
RX PubMed=2777778; DOI=10.1016/s0021-9258(18)71592-5;
RA Liu X.-Q., Gillham N.W., Boynton J.E.;
RT "Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii.
RT Wild-type sequence, mutation to streptomycin resistance and dependence, and
RT function in Escherichia coli.";
RL J. Biol. Chem. 264:16100-16108(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hauser C.R., Boynton J.E., Gillham N.W.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [4]
RP PROTEIN SEQUENCE OF 2-9.
RC STRAIN=Arg7/cw15;
RX PubMed=12417713; DOI=10.1105/tpc.004341;
RA Yamaguchi K., Prieto S., Beligni M.V., Haynes P.A., McDonald W.H.,
RA Yates J.R. III, Mayfield S.P.;
RT "Proteomic characterization of the small subunit of Chlamydomonas
RT reinhardtii chloroplast ribosome: identification of a novel S1 domain-
RT containing protein and unusually large orthologs of bacterial S2, S3, and
RT S5.";
RL Plant Cell 14:2957-2974(2002).
RN [5]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
CC -!- FUNCTION: With S4 and S5 plays an important role in translational
CC accuracy. Located at the interface of the 30S and 50S subunits (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC {ECO:0000305}.
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DR EMBL; M29284; AAA84155.1; -; Genomic_DNA.
DR EMBL; AF061851; AAC16329.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50147.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00960.1; -; Genomic_DNA.
DR PIR; A34248; A34248.
DR RefSeq; NP_958416.1; NC_005353.1.
DR AlphaFoldDB; P14149; -.
DR SMR; P14149; -.
DR STRING; 3055.DAA00960; -.
DR PaxDb; P14149; -.
DR PRIDE; P14149; -.
DR GeneID; 2717036; -.
DR KEGG; cre:ChreCp060; -.
DR eggNOG; KOG1750; Eukaryota.
DR HOGENOM; CLU_104295_1_2_1; -.
DR InParanoid; P14149; -.
DR OrthoDB; 1542634at2759; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd03368; Ribosomal_S12; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR006032; Ribosomal_S12/S23.
DR InterPro; IPR005679; Ribosomal_S12_bac.
DR PANTHER; PTHR11652; PTHR11652; 1.
DR Pfam; PF00164; Ribosom_S12_S23; 1.
DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR PRINTS; PR01034; RIBOSOMALS12.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:12417713"
FT CHAIN 2..133
FT /note="30S ribosomal protein S12, chloroplastic"
FT /id="PRO_0000146396"
FT MUTAGEN 14
FT /note="K->T: Streptomycin resistance."
FT MUTAGEN 91
FT /note="P->L: Streptomycin dependence."
SQ SEQUENCE 133 AA; 14603 MW; 22A4A5B782EAD62B CRC64;
MPTIQQLIRS ARKKITKKTK SPALKSCPQR RGICLRVYTV TPKKPNSALR KVARVRLTTG
FEVTAYIPGV GHNLQEHAVV LVRGGRVKDL PGVRYHIVRG SLDTAGVKNR VQSRSKYGVK
MGSKTAAKTA GKK
