ID   RR12_NICPL              Reviewed;         123 AA.
AC   P62127; P06369; P28806; P56803; Q9XQZ8;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=30S ribosomal protein S12, chloroplastic;
GN   Name=rps12; Synonyms=rps12-3;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND STREPTOMYCIN RESISTANT MUTANT.
RC   STRAIN=cv. Viviani; TISSUE=Leaf;
RX   PubMed=8219048; DOI=10.1007/bf00021429;
RA   Hsu C.-M., Yang W.-P., Chen C.-C., Lai Y.-K., Lin T.-Y.;
RT   "A point mutation in the chloroplast rps12 gene from Nicotiana
RT   plumbaginifolia confers streptomycin resistance.";
RL   Plant Mol. Biol. 23:179-183(1993).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. Located at the interface of the 30S and 50S subunits (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Exons 2 and 3 are cis-spliced, while a trans-splicing
CC       reaction is required to link exons 1 and 2. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
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DR   EMBL; L12366; AAA16345.1; -; Unassigned_DNA.
DR   EMBL; L12250; AAA16344.1; -; Unassigned_DNA.
DR   PIR; S39501; S39501.
DR   AlphaFoldDB; P62127; -.
DR   SMR; P62127; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_S12/S23.
DR   InterPro; IPR005679; Ribosomal_S12_bac.
DR   PANTHER; PTHR11652; PTHR11652; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Chloroplast; Plastid; Ribonucleoprotein;
KW   Ribosomal protein; RNA-binding; rRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..123
FT                   /note="30S ribosomal protein S12, chloroplastic"
FT                   /id="PRO_0000146410"
FT   MUTAGEN         88
FT                   /note="K->R: Streptomycin resistance in strain SR1018."
SQ   SEQUENCE   123 AA;  13764 MW;  E15ADC4952508F57 CRC64;
     MPTIKQLIRN TRQPIRNVTK SPALRGCPQR RGTCTRVYTI TPKKPNSALR KVARVRLTSG
     FEITAYIPGI GHNLQEHSVV LVRGGRVKDL PGVRYHIVRG TLDAVGVKDR QQGRSKYGVK
     KPK