ID   RR12_TOBAC              Reviewed;         123 AA.
AC   P62129; P06369; P28806; P56803; Q9XQZ8;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=30S ribosomal protein S12, chloroplastic;
GN   Name=rps12-A;
GN   and
GN   Name=rps12-B;
OS   Nicotiana tabacum (Common tobacco).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Bright Yellow 4;
RX   PubMed=16453699; DOI=10.1002/j.1460-2075.1986.tb04464.x;
RA   Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N.,
RA   Matsubayashi T., Zaita N., Chunwongse J., Obokata J.,
RA   Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B.-Y., Sugita M.,
RA   Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A.,
RA   Tohdoh N., Shimada H., Sugiura M.;
RT   "The complete nucleotide sequence of the tobacco chloroplast genome: its
RT   gene organization and expression.";
RL   EMBO J. 5:2043-2049(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
RX   PubMed=3960739; DOI=10.1093/nar/14.7.3143;
RA   Torazawa K., Hayashida N., Obokata J., Shinozaki K., Sugiura M.;
RT   "The 5' part of the gene for ribosomal protein S12 is located 30 kbp
RT   downstream from its 3' part in tobacco chloroplast genome.";
RL   Nucleic Acids Res. 14:3143-3143(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-123.
RX   PubMed=3945556; DOI=10.1093/nar/14.2.883;
RA   Fromm H., Edelman M., Koller B., Goloubinoff P., Galun E.;
RT   "The enigma of the gene coding for ribosomal protein S12 in the
RT   chloroplasts of Nicotiana.";
RL   Nucleic Acids Res. 14:883-898(1986).
RN   [4]
RP   STREPTOMYCIN RESISTANT MUTANT.
RC   STRAIN=cv. Petit Havana;
RX   PubMed=1356049; DOI=10.2307/3869380;
RA   Staub J.M., Maliga P.;
RT   "Long regions of homologous DNA are incorporated into the tobacco plastid
RT   genome by transformation.";
RL   Plant Cell 4:39-45(1992).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. Located at the interface of the 30S and 50S subunits (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Exons 2 and 3 are cis-spliced, while a trans-splicing
CC       reaction is required to link exons 1 and 2.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z00044; CAA77436.1; -; Genomic_DNA.
DR   EMBL; Z00044; CAA77429.1; -; Genomic_DNA.
DR   EMBL; X03724; CAA27361.1; -; Genomic_DNA.
DR   EMBL; X03481; CAA27200.1; -; Genomic_DNA.
DR   PIR; A02730; R3NT12.
DR   RefSeq; NP_054549.2; NC_001879.2.
DR   RefSeq; NP_054568.2; NC_001879.2.
DR   AlphaFoldDB; P62129; -.
DR   SMR; P62129; -.
DR   GeneID; 1466311; -.
DR   GeneID; 800423; -.
DR   KEGG; nta:1466311; -.
DR   KEGG; nta:800423; -.
DR   OrthoDB; 1542634at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00403_B; Ribosomal_S12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_S12/S23.
DR   InterPro; IPR005679; Ribosomal_S12_bac.
DR   PANTHER; PTHR11652; PTHR11652; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00981; rpsL_bact; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Chloroplast; Plastid; Ribonucleoprotein;
KW   Ribosomal protein; RNA-binding; rRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..123
FT                   /note="30S ribosomal protein S12, chloroplastic"
FT                   /id="PRO_0000146430"
FT   MUTAGEN         91
FT                   /note="P->S: Streptomycin resistance."
SQ   SEQUENCE   123 AA;  13764 MW;  E15ADC4952508F57 CRC64;
     MPTIKQLIRN TRQPIRNVTK SPALRGCPQR RGTCTRVYTI TPKKPNSALR KVARVRLTSG
     FEITAYIPGI GHNLQEHSVV LVRGGRVKDL PGVRYHIVRG TLDAVGVKDR QQGRSKYGVK
     KPK