AURKB_XENTR
ID AURKB_XENTR Reviewed; 360 AA.
AC A4IGM9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Aurora kinase B;
DE EC=2.7.11.1;
DE AltName: Full=Aurora/IPL1-related kinase 2;
DE Short=AIRK2;
DE Short=XAIRK2;
DE AltName: Full=Serine/threonine-protein kinase 12 {ECO:0000250|UniProtKB:Q96GD4};
DE AltName: Full=Serine/threonine-protein kinase aurora-B;
DE Short=xAurora-B;
GN Name=aurkb {ECO:0000312|EMBL:AAI35174.1}; Synonyms=airk2;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000312|EMBL:AAI35174.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tadpole {ECO:0000312|EMBL:AAI35174.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Serine/threonine-protein kinase component of the chromosomal
CC passenger complex (CPC), a complex that acts as a key regulator of
CC mitosis. The CPC complex has essential functions at the centromere in
CC ensuring correct chromosome alignment and segregation and is required
CC for chromatin-induced microtubule stabilization and spindle assembly.
CC Involved in the bipolar attachment of spindle microtubules to
CC kinetochores and is a key regulator for the onset of cytokinesis during
CC mitosis. Required for central/midzone spindle assembly and cleavage
CC furrow formation. Key component of the cytokinesis checkpoint, a
CC process required to delay abscission to prevent both premature
CC resolution of intercellular chromosome bridges and accumulation of DNA
CC damage. Phosphorylates 'Ser-10' of histone H3 during mitosis.
CC {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000250|UniProtKB:Q96GD4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000250|UniProtKB:Q96GD4};
CC -!- ACTIVITY REGULATION: Kinase activity is stimulated by cell-cycle
CC specific phosphorylation. {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SUBUNIT: Component of the chromosomal passenger complex (CPC).
CC {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96GD4}.
CC Chromosome {ECO:0000250|UniProtKB:Q96GD4}. Chromosome, centromere
CC {ECO:0000250|UniProtKB:Q96GD4}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q96GD4}. Midbody {ECO:0000250|UniProtKB:Q96GD4}.
CC Note=Localizes on chromosome arms and inner centromeres from prophase
CC through metaphase and then transferring to the spindle midzone and
CC midbody from anaphase through cytokinesis. Localization (and probably
CC targeting of the CPC) to the inner centromere occurs predominantly in
CC regions with overlapping mitosis-specific histone phosphorylations
CC H3pT3 and H2ApT12. {ECO:0000250|UniProtKB:Q96GD4}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. Aurora subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00159}.
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DR EMBL; BC135173; AAI35174.1; -; mRNA.
DR RefSeq; NP_001016859.2; NM_001016859.3.
DR RefSeq; XP_012809282.1; XM_012953828.2.
DR AlphaFoldDB; A4IGM9; -.
DR SMR; A4IGM9; -.
DR STRING; 8364.ENSXETP00000019956; -.
DR PaxDb; A4IGM9; -.
DR DNASU; 549613; -.
DR Ensembl; ENSXETT00000019956; ENSXETP00000019956; ENSXETG00000009097.
DR GeneID; 549613; -.
DR KEGG; xtr:549613; -.
DR CTD; 9212; -.
DR Xenbase; XB-GENE-1019626; aurkb.
DR eggNOG; KOG0580; Eukaryota.
DR HOGENOM; CLU_000288_63_0_1; -.
DR InParanoid; A4IGM9; -.
DR OrthoDB; 954262at2759; -.
DR Reactome; R-XTR-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-XTR-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-XTR-2467813; Separation of Sister Chromatids.
DR Reactome; R-XTR-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-XTR-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-XTR-5663220; RHO GTPases Activate Formins.
DR Reactome; R-XTR-9648025; EML4 and NUDC in mitotic spindle formation.
DR Proteomes; UP000008143; Chromosome 3.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000009097; Expressed in 2-cell stage embryo and 11 other tissues.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005694; C:chromosome; ISS:UniProtKB.
DR GO; GO:0032133; C:chromosome passenger complex; ISS:UniProtKB.
DR GO; GO:0000775; C:chromosome, centromeric region; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005876; C:spindle microtubule; IBA:GO_Central.
DR GO; GO:0051233; C:spindle midzone; IBA:GO_Central.
DR GO; GO:0031616; C:spindle pole centrosome; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0035175; F:histone kinase activity (H3-S10 specific); ISS:UniProtKB.
DR GO; GO:0035174; F:histone serine kinase activity; IBA:GO_Central.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
DR GO; GO:0009838; P:abscission; ISS:UniProtKB.
DR GO; GO:0034644; P:cellular response to UV; ISS:UniProtKB.
DR GO; GO:0036089; P:cleavage furrow formation; ISS:UniProtKB.
DR GO; GO:0043988; P:histone H3-S28 phosphorylation; IEA:InterPro.
DR GO; GO:0044878; P:mitotic cytokinesis checkpoint signaling; ISS:UniProtKB.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IEA:InterPro.
DR GO; GO:0051256; P:mitotic spindle midzone assembly; ISS:UniProtKB.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR GO; GO:0002903; P:negative regulation of B cell apoptotic process; ISS:UniProtKB.
DR GO; GO:0032466; P:negative regulation of cytokinesis; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
DR GO; GO:0062033; P:positive regulation of mitotic sister chromatid segregation; ISS:UniProtKB.
DR GO; GO:0043687; P:post-translational protein modification; ISS:UniProtKB.
DR GO; GO:0034501; P:protein localization to kinetochore; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0032465; P:regulation of cytokinesis; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR030616; Aur.
DR InterPro; IPR028772; AURKB.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24350; PTHR24350; 1.
DR PANTHER; PTHR24350:SF4; PTHR24350:SF4; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Cytoplasm; Cytoskeleton; Kinase; Mitosis;
KW Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..360
FT /note="Aurora kinase B"
FT /id="PRO_0000385449"
FT DOMAIN 93..343
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 216
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 99..107
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 122
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 360 AA; 41390 MW; 5B970C8A48C02A1E CRC64;
MSYKENLNPS SYTSKFATPS SATAAQRVLR KEPYVSTFTT PSDNLLAQRA QLARITPSAS
SSVPGRVAVS MDASSQNTAL AELPKRKFTI DDFDIGRPLG KGKFGNVYLA RDKQNKFIMA
LKVLFKSQLE KEGVEHQLRR EIEIQSHLRH PNILRMYNYF HDRKRIYLML EFAPRGELYK
ELQKHGRFDE QRSATFMEEL ADALQYCHER KVIHRDIKPE NLLMGYKGEL KIADFGWSVH
APSLRRRTMC GTLDYLPPEM IEGKTHDEKV DLWCAGVLCF EFLVGMPPFD SPSHTETHRR
IVNVDLKFPP FLSDGSKDLI SKLLRYHPPQ RLPLKGVMEH PWVKANSRRV LPPVFQSSSK
