AUS1_YEAST
ID AUS1_YEAST Reviewed; 1394 AA.
AC Q08409; D6W278; O94147;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=ATP-dependent permease AUS1;
GN Name=AUS1; OrderedLocusNames=YOR011W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=12077145; DOI=10.1074/jbc.m204707200;
RA Wilcox L.J., Balderes D.A., Wharton B., Tinkelenberg A.H., Rao G.,
RA Sturley S.L.;
RT "Transcriptional profiling identifies two members of the ATP-binding
RT cassette transporter superfamily required for sterol uptake in yeast.";
RL J. Biol. Chem. 277:32466-32472(2002).
CC -!- FUNCTION: Transporter involved in the uptake of sterol.
CC {ECO:0000269|PubMed:12077145}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCG family.
CC PDR (TC 3.A.1.205) subfamily. {ECO:0000305}.
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DR EMBL; Z74919; CAA99199.1; -; Genomic_DNA.
DR EMBL; Z74920; CAA99202.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10794.1; -; Genomic_DNA.
DR PIR; S66876; S66876.
DR RefSeq; NP_014654.1; NM_001183430.1.
DR AlphaFoldDB; Q08409; -.
DR SMR; Q08409; -.
DR BioGRID; 34416; 82.
DR DIP; DIP-994N; -.
DR IntAct; Q08409; 41.
DR MINT; Q08409; -.
DR STRING; 4932.YOR011W; -.
DR TCDB; 3.A.1.205.15; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q08409; -.
DR PRIDE; Q08409; -.
DR EnsemblFungi; YOR011W_mRNA; YOR011W; YOR011W.
DR GeneID; 854175; -.
DR KEGG; sce:YOR011W; -.
DR SGD; S000005537; AUS1.
DR VEuPathDB; FungiDB:YOR011W; -.
DR eggNOG; KOG0065; Eukaryota.
DR GeneTree; ENSGT00940000176496; -.
DR HOGENOM; CLU_000604_35_0_1; -.
DR InParanoid; Q08409; -.
DR OMA; GMIYFEV; -.
DR BioCyc; YEAST:G3O-33561-MON; -.
DR PRO; PR:Q08409; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08409; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0034041; F:ABC-type sterol transporter activity; IMP:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0035376; P:sterol import; IMP:SGD.
DR CDD; cd03233; ABCG_PDR_domain1; 1.
DR CDD; cd03232; ABCG_PDR_domain2; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013525; ABC_2_trans.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR043926; ABCG_dom.
DR InterPro; IPR034001; ABCG_PDR_1.
DR InterPro; IPR034003; ABCG_PDR_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010929; PDR_CDR_ABC.
DR Pfam; PF01061; ABC2_membrane; 2.
DR Pfam; PF19055; ABC2_membrane_7; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR Pfam; PF06422; PDR_CDR; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Membrane; Nucleotide-binding; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1394
FT /note="ATP-dependent permease AUS1"
FT /id="PRO_0000093434"
FT TOPO_DOM 1..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 497..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 558..575
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 636..658
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 659..1080
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1081..1103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1107..1129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1156..1178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1193..1215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1224..1246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1346..1368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1369..1394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 33..273
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 751..978
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 782..789
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ SEQUENCE 1394 AA; 157751 MW; 357A35A0FCF0C93B CRC64;
MSISKYFTPV ADGSLTFNGA NIQFGADAQG ESKKSYDAED SMPNPANQLN DITFQAEAGE
MVLVLGYPTS TLFKTLFHGK TSLSYSPPGS IKFKNNEFKS FSEKCPHQII YNNEQDVHFP
FLTVEQTIDF ALSCKFDIPK GERDQIRNEL LREFGLSHVL KTIVGNDFFR GVSGGERKRI
SIIETFIANG SVYLWDNSTK GLDSATALDF LEILRKMAKA TRSVNLVRIS QASDKIVDKF
DKILMLSDSY QLFYGTVDEC LTYFRDTLGI EKDPNDCIIE YLTSILNFQF KNKNLGNLSN
SSSASVLKTA TGEVTKYTYN SDFDLYDQWK HSSYYRNIKQ QIQGSSIDDS IKEVDPSDVS
PIFNIPLKKQ LLFCTKRAFQ RSLGDKAYMT AQFISVVIQS LVIGSLFYEI PLTTIGSYSR
GSLTFFSILF FTFLSLADMP IAFQRQPVVK KQSQLHFYTN WVETLSTTVF DYCFKLCLVI
VFSIILYFLA HLQYKAARFF IFLLFLSFYN FCMVSLFALT TLVAPTISVA NLFAGILLLA
IAMYASYVIY LKNMHPWFVW IAYLNPAMYA MEAILSNELY NLKLDCSETI VPRGPTYNDV
PFSHKACAWQ GATLGNDYVR GRDYLKQGLS YTYHHVWRNF GIIIGFLVFF IACTLFASQY
IKPYFNKDEI ERNNSRLTRW LPFLNKKRGT RSSARNDSKY VGIPKSHSVS SSSSSLSAVP
YQISPSNKEM ALNDYNEQPI TETVETQKHI ISWKNINYTV GTKKLINNAS GFISSGLTAL
MGESGAGKTT LLNVLSQRVE TGVVSGEILI DGHPLTDEDA FKRSIGFVQQ QDLHLDLLSV
KESLEISCLL RGDGDRAYLD TVSNLLKLPS DILVADLNPT QRKLLSIGVE LVTKPSLLLF
LDEPTSGLDA EAALTIVKFL KQLSLQGQAI FCTIHQPSKS VISHFDNIFL LKRGGECVFF
GPMDDACGYF MSHDNTLVYD KEHDNPADFV IDAVGNSNSS AGKDTAEEAL TLNKEAIDWS
ALWESSVEKK LVKKETARLE DDARASGVDY TTSLWKQPSY LQQLALITRR QYICTKRDMT
YVMAKYCLNG GAGLFIGFSF WHIKHNIIGL QDSIFFCFMA LCVSSPLINQ IQDKALKTKE
VYVAREARSN TYHWTVLLLS QSIIELPLAL TSSTLFFVCA FFSCGFNNAG WSAGVFFLNY
MLFAAYYSTL GLWLIYTAPN LQTAAVFVAF IYSFTASFCG VMQPYSLFPT FWKFMYRVSP
YTYFVETFVS ILLHNWEIKC DMSEMVPGQP LTGQSCGQFM EAFIEEYGGY LHNKNTFTVC
AYCTYTVGDD FLKNENMSYD HVWRNFGIEW AFVGFNFFAM FAGYYLTYVA RIWPKVFKII
TKVIPHRGKK PVQN
