RR31_SPIOL
ID RR31_SPIOL Reviewed; 101 AA.
AC P47910; A0A0K9RMU8; Q9M581;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=30S ribosomal protein S31, chloroplastic {ECO:0000303|PubMed:10874039};
DE AltName: Full=Chloroplastic small ribosomal subunit protein bTHXc {ECO:0000303|PubMed:28007896};
DE AltName: Full=Plastid-specific 30S ribosomal protein 4;
DE Short=PSRP-4;
DE Flags: Precursor;
GN Name=RPS31; Synonyms=PSRP4; ORFNames=SOVF_048530;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 55-97, SUBUNIT, AND
RP SUBCELLULAR LOCATION.
RX PubMed=12605670; DOI=10.1046/j.1432-1033.2003.03359.x;
RA Yamaguchi K., Subramanian A.R.;
RT "Proteomic identification of all plastid-specific ribosomal proteins in
RT higher plant chloroplast 30S ribosomal subunit.";
RL Eur. J. Biochem. 270:190-205(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [3]
RP PROTEIN SEQUENCE OF 55-97, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874039; DOI=10.1074/jbc.m004350200;
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 30S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28455-28465(2000).
RN [4]
RP PROTEIN SEQUENCE OF 55-95.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=8490565;
RA Schmidt J., Srinivasa B., Wegloehner W., Subramanian A.R.;
RT "A small novel chloroplast ribosomal protein (S31) that has no apparent
RT counterpart in the E. coli ribosome.";
RL Biochem. Mol. Biol. Int. 29:25-31(1993).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874039,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast small ribosomal subunit (SSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874039,
CC ECO:0000269|PubMed:12605670, ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874039, ECO:0000269|PubMed:12605670,
CC ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=5174; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: Mass=5175; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bTHX family.
CC {ECO:0000305}.
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DR EMBL; AF236825; AAF64154.1; -; mRNA.
DR EMBL; KQ138172; KNA20845.1; -; Genomic_DNA.
DR PIR; A48391; A48391.
DR PDB; 5MMJ; EM; 3.65 A; x=1-101.
DR PDB; 5MMM; EM; 3.40 A; x=1-101.
DR PDB; 5X8P; EM; 3.40 A; x=55-101.
DR PDB; 5X8R; EM; 3.70 A; x=55-101.
DR PDB; 6ERI; EM; 3.00 A; BW=55-91.
DR PDBsum; 5MMJ; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8R; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P47910; -.
DR SMR; P47910; -.
DR STRING; 3562.P47910; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IBA:GO_Central.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032544; P:plastid translation; IBA:GO_Central.
DR DisProt; DP01610; -.
DR InterPro; IPR030826; Ribo_THX.
DR InterPro; IPR044695; RPS31_plant.
DR PANTHER; PTHR34550; PTHR34550; 1.
DR Pfam; PF17067; RPS31; 1.
DR TIGRFAMs; TIGR04560; ribo_THX; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874039,
FT ECO:0000269|PubMed:8490565"
FT CHAIN 55..101
FT /note="30S ribosomal protein S31, chloroplastic"
FT /id="PRO_0000217198"
FT REGION 27..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 59..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 89
FT /note="P -> A (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 92
FT /note="P -> F (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 101 AA; 10585 MW; E93A6B76509414C3 CRC64;
MASLLLGTPS MACHSVSFSF SHSQSVAGVS LSSPPTLSLS SSVSTSPALP LIYCGRGDRK
TAKGKRFNHS FGNARPKNKN KGRGPPKAPI FPKGDPSQKE D
