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AUSU_EMENI
ID   AUSU_EMENI              Reviewed;         148 AA.
AC   A0A1U8QGE6; C8VQ91; Q5AR26;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Iron/alpha-ketoglutarate-dependent dioxygenase ausU {ECO:0000303|PubMed:29076725};
DE            EC=1.14.-.- {ECO:0000305|PubMed:29076725};
DE   AltName: Full=Austinoid biosynthesis clusters protein U {ECO:0000303|PubMed:29076725};
GN   Name=ausU {ECO:0000303|PubMed:29076725}; ORFNames=AN9254, ANIA_09254;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [3]
RP   FUNCTION.
RX   PubMed=22234162; DOI=10.1021/cb200455u;
RA   Rodriguez-Urra A.B., Jimenez C., Nieto M.I., Rodriguez J., Hayashi H.,
RA   Ugalde U.;
RT   "Signaling the induction of sporulation involves the interaction of two
RT   secondary metabolites in Aspergillus nidulans.";
RL   ACS Chem. Biol. 7:599-606(2012).
RN   [4]
RP   FUNCTION.
RX   PubMed=22329759; DOI=10.1021/ja209809t;
RA   Lo H.C., Entwistle R., Guo C.J., Ahuja M., Szewczyk E., Hung J.H.,
RA   Chiang Y.M., Oakley B.R., Wang C.C.;
RT   "Two separate gene clusters encode the biosynthetic pathway for the
RT   meroterpenoids austinol and dehydroaustinol in Aspergillus nidulans.";
RL   J. Am. Chem. Soc. 134:4709-4720(2012).
RN   [5]
RP   FUNCTION.
RX   PubMed=23865690; DOI=10.1021/ja405518u;
RA   Matsuda Y., Awakawa T., Wakimoto T., Abe I.;
RT   "Spiro-ring formation is catalyzed by a multifunctional dioxygenase in
RT   austinol biosynthesis.";
RL   J. Am. Chem. Soc. 135:10962-10965(2013).
RN   [6]
RP   FUNCTION.
RX   PubMed=29076725; DOI=10.1021/acschembio.7b00814;
RA   Mattern D.J., Valiante V., Horn F., Petzke L., Brakhage A.A.;
RT   "Rewiring of the austinoid biosynthetic pathway in filamentous fungi.";
RL   ACS Chem. Biol. 12:2927-2933(2017).
CC   -!- FUNCTION: Iron/alpha-ketoglutarate-dependent dioxygenase; part of the
CC       gene cluster B that mediates the biosynthesis of austinol and
CC       dehydroaustinol, two fungal meroterpenoids (PubMed:22329759). The first
CC       step of the pathway is the synthesis of 3,5-dimethylorsellinic acid by
CC       the polyketide synthase ausA (PubMed:22329759). 3,5-dimethylorsellinic
CC       acid is then prenylated by the polyprenyl transferase ausN
CC       (PubMed:22329759). Further epoxidation by the FAD-dependent
CC       monooxygenase ausM and cyclization by the probable terpene cyclase ausL
CC       lead to the formation of protoaustinoid A (PubMed:22329759).
CC       Protoaustinoid A is then oxidized to spiro-lactone preaustinoid A3 by
CC       the combined action of the FAD-binding monooxygenases ausB and ausC,
CC       and the dioxygenase ausE (PubMed:22329759, PubMed:23865690). Acid-
CC       catalyzed keto-rearrangement and ring contraction of the tetraketide
CC       portion of preaustinoid A3 by ausJ lead to the formation of
CC       preaustinoid A4 (PubMed:22329759). The aldo-keto reductase ausK, with
CC       the help of ausH, is involved in the next step by transforming
CC       preaustinoid A4 into isoaustinone which is in turn hydroxylated by the
CC       P450 monooxygenase ausI to form austinolide (PubMed:22329759). Finally,
CC       the cytochrome P450 monooxygenase ausG modifies austinolide to austinol
CC       (PubMed:22329759). Austinol can be further modified to dehydroaustinol
CC       which forms a diffusible complex with diorcinol that initiates
CC       conidiation (PubMed:22234162, PubMed:22329759). Due to genetic
CC       rearrangements of the clusters and the subsequent loss of some enzymes,
CC       the end products of the Emericella nidulans austinoid biosynthesis
CC       clusters are austinol and dehydroaustinol, even if additional enzymes,
CC       such as the O-acetyltransferase ausQ and the cytochrome P450
CC       monooxygenase ausR are still functional (PubMed:29076725).
CC       {ECO:0000269|PubMed:22234162, ECO:0000269|PubMed:22329759,
CC       ECO:0000269|PubMed:23865690, ECO:0000269|PubMed:29076725}.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000250|UniProtKB:Q5AR53};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305|PubMed:29076725}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q4WAW9}.
CC   -!- MISCELLANEOUS: In A.calidoustus, the austinoid gene cluster lies on a
CC       contiguous DNA region, while clusters from E.nidulans and P.brasilianum
CC       are split in their respective genomes. Genetic rearrangements provoked
CC       variability among the clusters and E.nidulans produces the least number
CC       of austionoid derivatives with the end products austinol and
CC       dehydroaustinol, while P.brasilianum can produce until
CC       acetoxydehydroaustin, and A.calidoustus produces the highest number of
CC       identified derivatives. {ECO:0000305|PubMed:29076725}.
CC   -!- SIMILARITY: Belongs to the PhyH family. {ECO:0000305}.
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DR   EMBL; BN001308; CBF87254.1; -; Genomic_DNA.
DR   EMBL; AACD01000172; EAA66321.1; -; Genomic_DNA.
DR   RefSeq; XP_682523.1; XM_677431.1.
DR   EnsemblFungi; CBF87254; CBF87254; ANIA_09254.
DR   EnsemblFungi; EAA66321; EAA66321; AN9254.2.
DR   GeneID; 2867771; -.
DR   KEGG; ani:AN9254.2; -.
DR   VEuPathDB; FungiDB:AN9254; -.
DR   HOGENOM; CLU_1758786_0_0_1; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000000560; Chromosome VIII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR008775; Phytyl_CoA_dOase.
DR   Pfam; PF05721; PhyH; 1.
PE   3: Inferred from homology;
KW   Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT   CHAIN           1..148
FT                   /note="Iron/alpha-ketoglutarate-dependent dioxygenase ausU"
FT                   /id="PRO_0000453863"
FT   BINDING         45
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:Q5AR53"
FT   BINDING         47
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250|UniProtKB:Q5AR53"
SQ   SEQUENCE   148 AA;  16175 MW;  5C0372562099EF9E CRC64;
     MTIIPKRSYN VLTTCKAVFR DVGDYWLTTG NLRTTKPQSP AQGFHRDTLL YPVLQYQPAT
     SPSLIVTLLV SMTDATVANG ATRVILSSQN GRLLNTIGGP GRASRAKRWG HAGNPSAAAA
     RWWEAYESGT EYKTNATNFL HKMLASCS
 
 
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