AB6C_ARATH
ID AB6C_ARATH Reviewed; 1466 AA.
AC Q8VZZ4; Q9LK63;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=ABC transporter C family member 6;
DE Short=ABC transporter ABCC.6;
DE Short=AtABCC6;
DE EC=7.6.2.2;
DE AltName: Full=ATP-energized glutathione S-conjugate pump 8;
DE AltName: Full=Glutathione S-conjugate-transporting ATPase 8;
DE AltName: Full=Multidrug resistance-associated protein 8;
GN Name=ABCC6; Synonyms=MRP6, MRP8; OrderedLocusNames=At3g13090;
GN ORFNames=MJG19.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Forestier C.;
RT "AtMRP6, a full size ABC transporter from the multidrug resistance protein
RT subfamily.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-1441.
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [5]
RP GENE FAMILY.
RX PubMed=11855639; DOI=10.1007/s004250100661;
RA Martinoia E., Klein M., Geisler M., Bovet L., Forestier C.,
RA Kolukisaoglu H.U., Mueller-Roeber B., Schulz B.;
RT "Multifunctionality of plant ABC transporters -- more than just
RT detoxifiers.";
RL Planta 214:345-355(2002).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12430019; DOI=10.1007/s00425-002-0890-6;
RA Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D.,
RA Martinoia E., Schulz B.;
RT "Family business: the multidrug-resistance related protein (MRP) ABC
RT transporter genes in Arabidopsis thaliana.";
RL Planta 216:107-119(2002).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
CC -!- FUNCTION: Pump for glutathione S-conjugates. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + xenobioticSide 1 = ADP + phosphate +
CC xenobioticSide 2.; EC=7.6.2.2;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:12430019}.
CC -!- INDUCTION: By salicylic acid (SA). {ECO:0000269|PubMed:12430019}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCC family.
CC Conjugate transporter (TC 3.A.1.208) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01400.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY052368; AAL14776.1; -; mRNA.
DR EMBL; AP000375; BAB01400.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75295.1; -; Genomic_DNA.
DR RefSeq; NP_187916.3; NM_112148.4.
DR AlphaFoldDB; Q8VZZ4; -.
DR SMR; Q8VZZ4; -.
DR BioGRID; 5831; 2.
DR STRING; 3702.AT3G13090.1; -.
DR PaxDb; Q8VZZ4; -.
DR PRIDE; Q8VZZ4; -.
DR ProteomicsDB; 244628; -.
DR EnsemblPlants; AT3G13090.1; AT3G13090.1; AT3G13090.
DR GeneID; 820497; -.
DR Gramene; AT3G13090.1; AT3G13090.1; AT3G13090.
DR KEGG; ath:AT3G13090; -.
DR Araport; AT3G13090; -.
DR TAIR; locus:2090039; AT3G13090.
DR eggNOG; KOG0054; Eukaryota.
DR HOGENOM; CLU_000604_27_1_1; -.
DR InParanoid; Q8VZZ4; -.
DR OMA; VAACAWY; -.
DR OrthoDB; 138195at2759; -.
DR BioCyc; ARA:AT3G13090-MON; -.
DR PRO; PR:Q8VZZ4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VZZ4; baseline and differential.
DR Genevisible; Q8VZZ4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0008559; F:ABC-type xenobiotic transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR CDD; cd18579; ABC_6TM_ABCC_D1; 1.
DR CDD; cd18580; ABC_6TM_ABCC_D2; 1.
DR Gene3D; 1.20.1560.10; -; 2.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR044746; ABCC_6TM_D1.
DR InterPro; IPR044726; ABCC_6TM_D2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00664; ABC_membrane; 2.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF90123; SSF90123; 2.
DR PROSITE; PS50929; ABC_TM1F; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 2: Evidence at transcript level;
KW ATP-binding; Membrane; Nucleotide-binding; Reference proteome; Repeat;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1466
FT /note="ABC transporter C family member 6"
FT /id="PRO_0000226079"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 322..339
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 512..532
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 890..910
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 937..957
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 1026..1046
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 286..567
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 601..824
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 900..1182
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 1219..1453
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT REGION 840..876
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 842..863
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 636..643
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 1253..1260
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CONFLICT 243
FT /note="L -> V (in Ref. 1; AAL14776)"
FT /evidence="ECO:0000305"
FT CONFLICT 612
FT /note="D -> E (in Ref. 1; AAL14776)"
FT /evidence="ECO:0000305"
FT CONFLICT 809
FT /note="H -> N (in Ref. 1; AAL14776)"
FT /evidence="ECO:0000305"
FT CONFLICT 853
FT /note="L -> I (in Ref. 1; AAL14776)"
FT /evidence="ECO:0000305"
FT CONFLICT 1451
FT /note="L -> F (in Ref. 1; AAL14776)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1466 AA; 164367 MW; A09808FB31E5753A CRC64;
MENPIDSLLL QPIYLSVLSF FLNLVLLLIL FGSWLFKKRV ACEDTDAIMN EEFKHISFSY
NKLVLICCVS LSVFYSVLSL LSCLHWHTNG WPFLDLLLAA LTWGSISVYL FGRYTNSCEQ
KVLFLLRVWW VFFFVVSCYH LVVDFVLYKK QEMVSVHFVI SDLVGVCAGL FLCCSCLWKK
GEGERIDLLK EPLLSSAESS DNEEVTAPFS KAGILSRMSF SWMSPLITLG NEKIIDIKDV
PQLDRSDTTE SLFWIFRSKL EWDDGERRIT TFKLIKALFL SVWRDIVLSA LLAFVYTVSC
YVAPYLMDNF VQYLNGNRQY KNQGYVLVTT FFVAKLVECQ TQRQWFFRGQ KAGLGMRSVL
VSMIYEKGLT LPCHSKQGHT SGEIINLMAV DADRISAFSW FMHDPWILVL QVSLALWILY
KSLGLGSIAA FPATILVMLA NYPFAKLEEK FQSSLMKSKD NRMKKTSEVL LNMKILKLQG
WEMKFLSKIL ELRHIEAGWL KKFVYNSSAI NSVLWAAPSF ISATAFGACL LLKIPLESGK
ILAALATFRI LQGPIYKLPE TISMIVQTKV SLNRIASFLC LDDLQQDVVG RLPSGSSEMA
VEISNGTFSW DDSSPIPTLR DMNFKVSQGM NVAICGTVGS GKSSLLSSIL GEVPKISGNL
KVCGRKAYIA QSPWIQSGKV EENILFGKPM EREWYDRVLE ACSLNKDLEI LPFHDQTVIG
ERGINLSGGQ KQRIQIARAL YQDADIYLFD DPFSAVDAHT GSHLFKEVLL GLLRHKTVIY
VTHQVEFLPE ADLILVMKDG KITQAGKYHE ILDSGTDFME LVGAHTEALA TIDSCETGYA
SEKSTTDKEN EVLHHKEKQE NGSDNKPSGQ LVQEEEREKG KVGFTVYKKY MALAYGGAVI
PLILVVQVLF QLLSIGSNYW MTWVTPVSKD VEPPVSGFTL ILVYVLLAVA SSFCILIRAL
LVAMTGFKMA TELFTQMHLR IFRASMSFFD ATPMGRILNR ASTDQSVADL RLPGQFAYVA
IAAINILGII GVIVQVAWQV LIVFIPVVAA CAWYRQYYIS AARELARLAG ISRSPVVHHF
SETLSGITTI RSFDQEPRFR GDIMRLSDCY SRLKFHSTGA MEWLCFRLEL LSTFAFASSL
VILVSAPEGV INPSLAGLAI TYALNLNTLQ ATLIWTLCDL ENKMISVERM LQYTNIPSEP
PLVIETTRPE KSWPSRGEIT ICNLQVRYGP HLPMVLHGLT CTFPGGLKTG IVGRTGCGKS
TLIQTLFRIV EPAAGEIRID GINILSIGLH DLRSRLSIIP QDPTMFEGTI RSNLDPLEEY
TDDQIWEALD NCQLGDEVRK KELKLDSPVS ENGQNWSVGQ RQLVCLGRVL LKRSKLLVLD
EATASIDTAT DNLIQETLRH HFADCTVITI AHRISSVIDS DMVLLLDQGL IKEHDSPARL
LEDRSSLFSK LVAEYTTSSE SKSKRS
