AUSY_ASPCI
ID AUSY_ASPCI Reviewed; 614 AA.
AC A0A0U5GJZ5;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 1.
DT 03-AUG-2022, entry version 21.
DE RecName: Full=MFS-type transporter ausY {ECO:0000303|PubMed:28233494};
DE AltName: Full=Austinoid biosynthesis cluster protein Y {ECO:0000303|PubMed:28233494};
GN Name=ausY {ECO:0000303|PubMed:28233494}; ORFNames=ASPCAL14375;
OS Aspergillus calidoustus.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=454130;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SF006504;
RX PubMed=26966204; DOI=10.1128/genomea.00102-16;
RA Horn F., Linde J., Mattern D.J., Walther G., Guthke R., Scherlach K.,
RA Martin K., Brakhage A.A., Petzke L., Valiante V.;
RT "Draft genome sequences of fungus Aspergillus calidoustus.";
RL Genome Announc. 4:0-0(2016).
RN [2]
RP FUNCTION.
RX PubMed=28233494; DOI=10.1021/acschembio.7b00003;
RA Valiante V., Mattern D.J., Schueffler A., Horn F., Walther G.,
RA Scherlach K., Petzke L., Dickhaut J., Guthke R., Hertweck C., Nett M.,
RA Thines E., Brakhage A.A.;
RT "Discovery of an Extended Austinoid Biosynthetic Pathway in Aspergillus
RT calidoustus.";
RL ACS Chem. Biol. 12:1227-1234(2017).
RN [3]
RP FUNCTION.
RX PubMed=29076725; DOI=10.1021/acschembio.7b00814;
RA Mattern D.J., Valiante V., Horn F., Petzke L., Brakhage A.A.;
RT "Rewiring of the austinoid biosynthetic pathway in filamentous fungi.";
RL ACS Chem. Biol. 12:2927-2933(2017).
CC -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC the biosynthesis of calidodehydroaustin, a fungal meroterpenoid.
CC {ECO:0000269|PubMed:29076725}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- MISCELLANEOUS: In A.calidoustus, the austinoid gene cluster lies on a
CC contiguous DNA region, while clusters from E.nidulans and P.brasilianum
CC are split in their respective genomes. Genetic rearrangements provoked
CC variability among the clusters and E.nidulans produces the least number
CC of austionoid derivatives with the end products austinol and
CC dehydroaustinol, while P.brasilianum can produce until
CC acetoxydehydroaustin, and A.calidoustus produces the highest number of
CC identified derivatives. {ECO:0000305|PubMed:29076725}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
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DR EMBL; CDMC01000024; CEL11272.1; -; Genomic_DNA.
DR SMR; A0A0U5GJZ5; -.
DR EnsemblFungi; CEL11272; CEL11272; ASPCAL14375.
DR OrthoDB; 627633at2759; -.
DR Proteomes; UP000054771; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..614
FT /note="MFS-type transporter ausY"
FT /id="PRO_0000453876"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 577..597
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 15
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 614 AA; 65630 MW; A33DFCBF0A345E0C CRC64;
MQPSLLDRIS SVTGNRSKRH AKFRLGRLQQ PPTESTTRLA ISDGAQAIDE TKSPRQSEPG
ATQCKSEGSG DDIADGPQPM PAPNEGDTPT LDELQQGPNP RGSGIVSGWK LAAVLTALSL
AVFCMALDST VLSTAIPKIT AEFNSLHDMG WYVSAYNLTI SSFSLIYGKL YSLYRVKWVY
LSTLFLFEAG SLVCGAAPSS LALIIGRAIA GVGGAGVFLG SMIIVHEIMP LHHVPLFLSL
ISGLYGIASV VGPLLGGVFT DYVSWRWCFY INLPIGGVTA LIIVLFVRPR ARDQATVKKT
VWAQFLELDP LGMVLILPAV MCILLVLQWG GAKYAWSNWR IIVLLVMGGS LALTFTVVQL
WQGDRATIPP RIVRNRTVWG AIVFAVCLYG SFTVFTYYLP IWFQAIKSAS ATRSGIMNLP
MILGVVVCSM LSGWAVSRVG YYVPFMYMAP VVGSIGAGLL TTLHVSSGPA AWITYQLVLG
VGTGAGMALP LVAVQTALAA DDVSTGSAII TFTQTLAGAL FNFVAQSVFQ SRLVHNLNAA
LPGVDTARVA DASPMEIRSA IEPEYLPQAL DAYSVSITQV FYAGAALCAL AVFGVLPMKW
LSVKGSRIQP VAHA
